Anti gapdh hrp 60004

Fudosteine (Fud, purity ≥98%) was purchased from Feiyu Biological Technology (Nantong, Jiangsu, China). SU11274 (a selective HGF antagonist, purity ≥98%) was purchased from ApexBio (Houston, TX, USA). LPS was purchased from Sigma-Aldrich (St. Louis, MO, USA). TNF-α was purchased from Peprotech (Rocky Hill, USA). Anti-HGF (BS6234) was purchased from Bioworld (St. Paul, MN, USA). Anti-p-ERK (#4377S) and the HRP-coupled secondary antibody (#7074S or #7076S) were purchased from Cell Signal Technology (Beverly, MA, USA). Anti-MUC5AC (sc-21701) and anti-EGFR (sc-80543) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-CD11b (101206) was purchased from Biolegend (San Diego, CA, USA). Anti-ERK (51068-1-AP) and anti-GAPDH (HRP-60004) were purchased from Proteintech (Chicago, IL, USA). HGF, MUC5AC, IL-6 and IL-13 Enzyme-linked Immunosorbent Assay (ELISA) kits were purchased from Elabscience Biotechnology (Wuhan, Hubei, China). Phenol red was purchased from Maikun Chemical (Shanghai, China). Rotring ink was obtained from Rotring (Hamburg, Germany).

Tissue or cell lysates were prepared in RIPA buffer (Sigma-Aldrich). A bicinchoninic acid (BCA) assay (Pierce, Rockford, IL, USA) was used to quantify total protein. Subsequently, proteins were separated by SDS-PAGE and transferred to PVDF membranes. After blocking with 5% non-fat dried milk for 1 h at RT, the membranes were incubated with primary antibodies overnight at 4 °C, which was followed by incubation with a secondary antibody conjugated with horseradish peroxidase (HRP) for 1 h at RT. The chemiluminescence signal of immunoreactive proteins was obtained using the SuperSignal West Pico Chemiluminescence Substrate (Pierce). Images were visualized and captured with the G-Box gel documentation system (Syngene, Frederick, MD, USA) or on X-ray film. Densitometric quantification of the bands was performed with ImageJ 1.53e software (NIH, Bethesda, MD, USA).
The following primary antibodies were purchased from ProteinTech (Chicago, IL, USA): anti-PRDX1 (#15816-1-AP), anti-PRDX2 (#10545-2-AP), anti-β-actin (#81115-1-RR), and anti-GAPDH (#HRP-60004). The antibody against LC3 (#L7643) and the secondary anti-rabbit antibody (#A0545) were obtained from Sigma-Aldrich.

Following SDS‒PAGE using Bis–Tris 10% gels (Thermo Fisher Scientific, MA, USA), proteins were directly blotted onto PVDF membranes. Subsequently, the membranes were blocked with RotiBlock (Roth, Germany) and incubated with the following antibodies: anti-ABCC2 (12559; CST, Danvers, MA, USA), anti-ABCG2 (42078; CST, Danvers, MA, USA), anti-ATF4 (10835; Proteintech), anti-ATF6 (15794; Proteintech), anti-BIM (2933; CST, Danvers, MA, USA), anti-BiP (Grp78; 610978; BD Biosciences), anti-cleaved caspase-3, -7, -9, (9644; 8438; 7237; all CST, Danvers, MA, USA) anti-CHOP (Gadd 153; sc-793; Santa Cruz, USA), anti-phospho-IRE1α (phospho S724; ab124945; abcam), anti-LC3B (3868; CST, Danvers, MA, USA), anti-MCL-1 (4572; CST, Danvers, MA, USA) anti-PDI (610946; BD Biosciences), anti-NOXA (OP180; Calbiochem), anti-polyUb (PW 8805-0500; Enzo, NY, USA), and anti-GAPDH (hrp-60004; Proteintech).