Ix70 inverted light microscope

Manufactured by Olympus

Sourced in Japan

The IX70 inverted light microscope is a versatile and high-performance instrument designed for a variety of imaging applications. It features a stable and ergonomic design, with a motorized focusing system and advanced optics to provide clear and detailed images. The IX70 is capable of various imaging modes, including brightfield, phase contrast, and fluorescence microscopy.

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Lab products found in correlation

Cellsens dimension software, by Olympus (1 mentions) Matlab, by MathWorks (1 mentions) Dp72 camera, by Olympus (1 mentions) Alizarin red s, by Cyagen (1 mentions) Dp71 digital camera, by Olympus (1 mentions) Pkh67 dye, by Merck Group (1 mentions) Bx51 light microscope, by Nikon (1 mentions)

Close spelling variants of this product

Inverted microscope (2417 citations) IX70 microscope (422 citations) Inverted light microscope (382 citations) IX70 inverted microscope (296 citations) IX70 light microscope (12 citations)

6 protocols using ix70 inverted light microscope

Automated Microscopic Analysis of Mucus Flakes

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Beads entrapped in mucus flakes were imaged via yellow-green fluorescence on an Olympus IX-70 inverted light microscope using an automated stage. Individual 2048×2048 pixel (210×210 μm) fields were imaged via phase contrast and yellow-green fluorescence with 10 ms exposures, and the imaging process was repeated using pre-programmed motion (CellSens Dimension software, ©2017 Olympus) for the entire slide. The image series was stitched together, and metrics regarding flake size and abundance obtained using a custom written Matlab program (© 2017 The Mathworks). The program outlined flake regions based on a user determined threshold that was modified to ensure optimal identification of visible flakes

Esther CR J.r., Muhlebach M.S., Ehre C., Hill D.B., Wolfgang M.C., Kesimer M., Ramsey K.A., Markovetz M.R., Garbarine I.C., Forest M.G., Seim I., Zorn B., Morrison C.B., Delion M.F., Thelin W.R., Villalon D., Sabater J.R., Turkovic L., Ranganathan S., Stick S.M, & Boucher R.C. (2019). Mucus accumulation in the lungs precedes structural changes and infection in children with cystic fibrosis. Science translational medicine, 11(486), eaav3488.

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Microscopic Imaging Protocol for Cellular Analysis

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Photomicrographs were obtained on an Olympus IX70 inverted light microscope and an Olympus DP72 camera using CellSens Standard 1.5 software. No images were manipulated with editing software.

Davis R.J., Silvin C, & Allen C.T. (2016). Avoiding phagocytosis-related artifact in myeloid derived suppressor cell T-lymphocyte suppression assays. Journal of immunological methods, 440, 12-18.

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Alizarin Red Staining for Osteogenesis

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Mineralization (calcium deposition) to evaluate the degree of osteogenesis on the samples was determined by Alizarin Red Staining. Samples were fixed in 4% paraformaldehyde solution for 15 min, rinsed with 0.01M PBS, stained with Alizarin Red S (Cyagen, USA) for 5 min and rinsed with 0.01M PBS before imaging. Images were collected with Olympus IX70 inverted light microscope (Japan) with a 20x objective.

Hasturk O., Ermis M., Demirci U., Hasirci N, & Hasirci V. (2019). Square prism micropillars on poly(methyl methacrylate) surfaces modulate the morphology and differentiation of human dental pulp mesenchymal stem cells. Colloids and surfaces. B, Biointerfaces, 178, 44-55.

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Live Cell Imaging of ATXN1 Aggregation

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DAOY cells stably-expressing RFP-ATXN1[82Q]-IRES-YFP [17 (link)] were grown on 35mm culture plates, transferred to RC-30HV imaging chambers (Warner Instruments 64-0321), heated to 37 °C using a dual automatic temperature controller (Warner TC-344C), and imaged for brightfield and RFP with an Olympus IX-70 inverted light microscope equipped with fluorescent optics and an Olympus DP71 digital camera. 10 nM staurosporine or DMSO vehicle were added to the plates at time zero; images were captured every 2 minutes and processed using Adobe Photoshop.

Pérez Ortiz J.M., Mollema N., Toker N., Adamski C.J., O’Callaghan B., Duvick L., Friedrich J., Walters M.A., Strasser J., Hawkinson J.E., Zoghbi H.Y., Henzler C., Orr H.T, & Lagalwar S. (2018). Reduction of Protein Kinase A-mediated Phosphorylation of ATXN1-S776 in Purkinje Cells Delays Onset of Ataxia in a SCA1 Mouse Model. Neurobiology of disease, 116, 93-105.

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Exosome Labeling and Imaging

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Exosomes were labeled using PKH67 dye (Sigma Aldrich) according to the manufacturer’s protocol. Labeled exosomes were suspended in PBS and added to target cells and imaged on an Olympus IX70 inverted light microscope as described previously^{17 (link)}.

Munson P.B., Hall E.M., Farina N.H., Pass H.I, & Shukla A. (2019). Exosomal miR-16-5p as a target for malignant mesothelioma. Scientific Reports, 9, 11688.

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Cyst Isolation and Germination Experiment

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This article is protected by copyright. All rights reserved. fraction was separated, using the density method (sodiumpolytungstate (SPT) at a density of 1.3 g cm -1 (Bolch 1997)).
Subsequently, single cysts were hand-picked and transferred to Nunclon 0.5-ml microwells at 20 °C filled with EMS medium (salinity 12 psu) (Watanabe et al. 2000) , at a light intensity of 100 µmol photons m -2 s -1 and 24 hours light; temperature and salinity were comparable to the SW Caspian Sea environment. The incubation experiment was initiated on the 9th of November 2011 and followed up until the 6th of December 2011.
Cysts were regularly checked for germination, and observations of the cells were performed under an Olympus IX70 inverted light microscope (Olympus, Tokyo, Japan). Encysted and excysted cysts and vegetative stages were photographed, using an Olympus BX51 light microscope with a Nikon digital sight DS-1L 1 module, all with 100x oil immersion objectives at 1000x magnification.
Kofoidean nomenclature is used to designate the plate numbers. We follow the interpretation of Ellegaard et al. (2002) of the first postcingular homolog as the small elongate plate bordering the sulcus. For simplicity, we chose not to use the 'para' terminology to distinguish features of cysts from the motile counterparts.

Mertens K.N., Takano Y., Gu H., Bagheri S., Pospelova V., Pieńkowski A.J., Leroy S.A.G, & Matsuoka K. (2017). Cyst-Theca Relationship and Phylogenetic Position of Impagidinium caspienense Incubated from Caspian Sea Surface Sediments: Relation to Gonyaulax baltica and Evidence for Heterospory within Gonyaulacoid Dinoflagellates. The Journal of eukaryotic microbiology, 64(6).

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