Trizol reagent 15596018

A reverse transcription kit (TAKARA, DRR037A) and real-time master kit (DRR014A) were purchased from Takara Bio Inc. (Otsu, Japan); Trizol reagent (15596018) and DNaseI (AM2235) were from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Antibodies against CD markers (CD3, CD4, CD14, CD19, CD16, CD56, CD20, CD123, HLA-DR, CD11c, and CD38) were purchased from the eBioscience company (San Diego, CA, USA). An IFN-α ELISA kit (3425-1H-6) was provided by the Mabtech company (Cincinnati, Ohio, USA), and recombinant human IFN-α and TNF-α were purchased from PeproTech Inc. (Westlake Village, CA, USA). CpG2216 oligonucleotides were synthesized by Thermo Fisher Scientific Inc. (Waltham, MA, USA). Ig subset antibodies for IgA1, IgA2, and IgG ELISA were purchased from Abcam Inc. (Cambridge, UK), LifeSpan BioSciences Inc. (Seattle, WA, USA), and DAKO Inc. (Santa Clara, CA, USA). Anti-IFN-α and anti-TLR9 antibody for immunofluorescence staining were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA) and Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA), respectively.