HPAF-II, AsPC-1, BxPC-3, CAPAN-1, CAPAN-2, CFPAC-1, Hs766T, MIAPaCa-2, Mpanc96, PANC-1, PSN-1, and SW1990 cells were obtained from ATCC (Manassas, VA, USA). Thirteen cell lines (KMP2, KMP3, KMP4, KMP5, KMP8, KP1L, KP1NL, KP2, KP3, KP3L, KP4, PH61N, and QGP-1) and normal human fibroblasts (WI-38, TIG-1, and IMR-90 cells) were obtained from JCRB cell bank (Tokyo, Japan). HPC-Y0, HPC-Y3, and HPC-Y25 were kindly given to us by Dr. Otsuji E (Kyoto Prefectural University of Medicine). PK-59 cells were obtained from RIKEN Bioresource Research Center (Ibaraki, Japan). All cell lines were cultured in RPMI-1640 medium or DMEM (Wako, Osaka, Japan) containing 10% fetal bovine serum (FBS) at 37°C with 5% CO2.
Wi 38
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in Japan
The WI-38 is a cell line derived from normal human embryonic lung tissue. It is a commonly used reference cell line in research and development applications.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Fujifilm (2 mentions)
Tig 1, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Imr 90, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Mcf 7, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Mia paca 2, by American Type Culture Collection (1 mentions)
Bxpc 3, by American Type Culture Collection (1 mentions)
Panc 1, by American Type Culture Collection (1 mentions)
Aspc 1, by American Type Culture Collection (1 mentions)
Hpaf 2, by American Type Culture Collection (1 mentions)
Capan 2, by American Type Culture Collection (1 mentions)
Sw1990, by American Type Culture Collection (1 mentions)
Capan 1, by American Type Culture Collection (1 mentions)
Qgp 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Cfpac 1, by American Type Culture Collection (1 mentions)
Hs766t, by American Type Culture Collection (1 mentions)
Psn 1, by American Type Culture Collection (1 mentions)
Mpanc96, by American Type Culture Collection (1 mentions)
Rpmi 1640 medium, by Fujifilm (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
4 protocols using wi 38
1
Characterization of Pancreatic Cancer Cell Lines
2
Epigenetic Regulation in Cancer Cell Lines
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Human osteosarcoma (U2OS and Saos-2), cervical carcinoma (HeLa), breast adenocarcinoma (MCF7 and MDA-MB-231), lung carcinoma (A549 and H1299) and normal human fibroblasts (TIG, MRC5 and WI38) were purchased from Japanese Collection of Research Bioresources (JCRB, Japan) and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL, Grand Island, NY, USA) supplemented with 10% (v/v) fetal bovine serum (Gibco BRL), and 1% (v/v) penicillin/streptomycin in the presence of 5% CO2 at 37 °C as described earlier30 (link)31 (link)32 (link)33 (link)34 (link)35 (link). Cells were treated with 20 μM of 5-Aza-2′deoxycytidine (5-Aza-dC) (Sigma-Aldrich, St. Louis, MO, USA) for 72–96 h and subjected to the following in vitro assays.
3
Cellular Internalization and DNA Damage
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Human normal (TIG-3, MRC5 and WI38) and cancer (breast carcinoma-MCF-7, melanoma-G361 and osteosarcoma-U2OS) cells were obtained from Japanese Collection of Research Bioresources (JCRB, Japan). The cells were authenticated by the source. Cells were frozen in -80 °C and LN2 in multiple vials and were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL, Grand Island, NY, USA) and treated either with Wi-A or Wi-N at about 60% confluency. Internalization of Wi-A and Wi-N was detected by immunostaining with the anti-WiNA Ab raised in our laboratory. The treated cells were also immuno-stained with a variety of other antibodies that included anti-γH2AX (Millipore #07-627), anti-ATR1 (Abcam, #ab4471), anti-CHK1 (Cell Signaling #2345S), anti-p53 (Santa Cruz. #sc-126) and anti-CARF antibody was raised in our laboratory63 (link).
4
Cell Culture Authentication Protocol
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A431 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and normal human fibroblasts (WI-38, TIG-1, and IMR-90 cells) were obtained from the JCRB cell bank (Tokyo, Japan). The cultures were maintained in DMEM (Wako, Tokyo, Japan) at 37°C with 5% CO2. Once resuscitated, the cell lines were authenticated by monitoring cell morphology.
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