Cbl171

Manufactured by Merck Group

Sourced in United States

CBL171 is a laboratory instrument designed for general use in scientific research and testing. The core function of this product is to provide precise measurement and analysis capabilities for various applications. Detailed specifications and intended use cases are not available.

Automatically generated - may contain errors

Lab products found in correlation

Knockout serum, by Thermo Fisher Scientific (1 mentions) Sc 281692, by Santa Cruz Biotechnology (1 mentions) Sox17, by R&D Systems (1 mentions) Knockout dmem f 12, by Thermo Fisher Scientific (1 mentions) Non essential amino acids 1x, by Thermo Fisher Scientific (1 mentions) Glutamax 1x, by Thermo Fisher Scientific (1 mentions) Es 006 b, by Merck Group (1 mentions) Aggrewell 800, by STEMCELL (1 mentions) Ab60721, by Abcam (1 mentions) Anti α sma, by Merck Group (1 mentions) Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions) Ab124734, by Abcam (1 mentions) Ab21286, by Abcam (1 mentions) F7387, by Merck Group (1 mentions) Zb 2305, by ZSGB-BIO (1 mentions) Ab92486, by Abcam (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Sc 13133, by Santa Cruz Biotechnology (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Ab27957, by Abcam (1 mentions)

6 protocols using cbl171

Differentiation of Mouse and Human EPS Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse and human EPS cells were trypsinized to single cells, separated from the MEF feeder cells by pre-plating on gelatin-coated plates, and cultured for 6 days on ultra-low attachment plates in IMDM (Thermo Fisher Scientific, 12440-053) supplemented with 15% FBS. Then, EBs were collected and plated on the Matrigel-coated plates for 6 days in the same medium, fixed and detected. For human cells, antibodies include anti-FOXA2 (1:200; Abcam, ab108422), anti-LHX5 (1:200; Santa Cruz, sc-130469) and anti-α-SMA (1:400; Millipore, CBL171). For mouse cells, the antibodies included anti-FOXA2 (1:200; ab60721; Abcam), anti-β-III TUBULIN (1:300; Santa Cruz, sc-80016) and anti-α-SMA (1:400; Millipore, CBL171).

Yang Y., Liu B., Xu J., Wang J., Wu J., Shi C., Xu Y., Dong J., Wang C., Lai W., Zhu J., Xiong L., Zhu D., Li X., Yang W., Yamauchi T., Sugawara A., Li Z., Sun F., Li X., Li C., He A., Du Y., Wang T., Zhao C., Li H., Chi X., Zhang H., Liu Y., Li C., Duo S., Yin M., Shen H., Belmonte J.C, & Deng H. (2017). Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency. Cell, 169(2), 243-257.e25.

+ Open protocol

+ Expand

Differentiation of Stem Cells into Germ Layers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Embryoid body (EB) formation was performed by plating single cells in AggreWell800 (Stem Cell Technologies, 34811, Vancouver, Canada) in medium containing Knockout DMEM F-12 (Gibco,12660-012, Grand Island, NY, USA), 20% Knockout Serum (Gibco, 10828-028, Grand Island, NY, USA), non-essential amino acids-1x (Gibco, 11140-050) and Glutamax-1x (Gibco, 35050-061). Medium was changed 48 hours later and thereafter till day 7 in an every-other-day mode. On day 7, EB spheres were collected and plated onto plates coated with 0.1% Gelatin (Millipore, ES-006-B) and medium containing Dulbecco’s modified Eagle’s medium (DMEM) (Gibco 11965-092), 20% fetal bovine serum (FBS) (Gibco, SH30071), non-essential amino acids-1x (Gibco, 11140-050) and Glutamax-1x (Gibco, 35050-061). Medium was changed every other day for 7 days. On day 7 post plating, EBs were fixed with 4% paraformaldehyde (Santa Cruz, SC-281692, Dallas, TX, USA), and stained for detection of cells of the three germ layers with antibodies for the following antigens: SOX17 (R&D Systems, AF1924, Minneapolis, MN, USA) for endoderm, PAX6 (BioLegend, PRB-278P, San Diego, CA, USA) for ectoderm and SMA (Millipore, CBL171) for mesoderm.

Pandey P.R., Tomney A., Woon M.T., Uth N., Shafighi F., Ngabo I., Vallabhaneni H., Levinson Y., Abraham E, & Friedrich Ben-Nun I. (2019). End-to-End Platform for Human Pluripotent Stem Cell Manufacturing. International Journal of Molecular Sciences, 21(1), 89.

+ Open protocol

+ Expand

Western Blot Analysis of Lung Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein lysates were extracted from lung tissues or MLg2908 cells by homogenization in the Laemmli sample buffer. An equal amount of proteins (30 μg per lane) were separated by electrophoresis on 8% polyacrylamide gels, and the proteins were electro-transferred onto polyvinylidene difluoride membranes (EMD Millipore, Billerica, MA, USA) overnight. The membranes were then incubated with primary antibodies as follows: anti-β-actin (1:1000, Santa Cruz), anti-α-smooth muscle actin (SMA) (1:1000, CBL171, Millipore), anti-transforming growth factor (TGF)-β1 (1:1000, ab92486, Abcam), anti-collagen type 1 (Col I) (1:1000, ab21286, Abcam), anti-fibronectin (FN) (1:1000, F7387, Sigma-Aldrich), anti-vitamin D receptor (VDR) (1:1000, sc-13133, Santa Cruz), anti-renin (1:1000, sc-133145, Santa Cruz), anti-AT1R (1:1000, ab124734, Abcam) and anti-AGT (1:1000, sc-374511, Santa Cruz). Secondary antibody was horseradish peroxidase-conjugated anti-IgG (ZB-2301, ZB-2305, ZSGB-BIO). The relative amount of proteins in each band was quantified using ImageJ (NIH), and normalized to β-actin (TA-09, ZSGB-BIO) as an internal loading control.

Chang J., Nie H., Ge X., Du J., Liu W., Li X., Sun Y., Wei X., Xun Z, & Li Y.C. (2021). Vitamin D suppresses bleomycin-induced pulmonary fibrosis by targeting the local renin–angiotensin system in the lung. Scientific Reports, 11, 16525.

+ Open protocol

+ Expand

Isolation and Cultivation of Rat Vascular Smooth Muscle Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rat vascular smooth muscle cells (VSMCs) were prepared by explantation of the descending thoracic aorta of 4-week-old male rats under anesthesia (10 % chloral hydrate; 350 mg/kg ip.) and were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10 % (v/v) fetal bovine serum as previously reported [18 (link)]. All of the cells were incubated at 37 °C in 5 % CO₂ in air. VSMCs were authenticated using immunohistochemical staining for α-smooth muscle actin (Millipore, CBL171). Cultures showing more than 95 % staining for α-actin between passage three and seven were used. All experimental procedures were performed according to the guidelines for the care and use of animals established by Soochow University and conformed to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised 1996).

Yu K.Y., Wang Y.P., Wang L.H., Jian Y., Zhao X.D., Chen J.W., Murao K., Zhu W., Dong L., Wang G.Q, & Zhang G.X. (2014). Mitochondrial KATP channel involvement in angiotensin II-induced autophagy in vascular smooth muscle cells. Basic Research in Cardiology, 109(4), 416.

+ Open protocol

+ Expand

Histological Analysis of Tissue Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

For histological analysis, tissues were dissected and fixed in a mix of 2% PFA and 20% sucrose solution overnight, then embedded in Tissue-tek O.C.T. (Electron Microscopy Sciences). Blocks were frozen in a dry ice and ethanol bath. For immunofluorescence, 6μm O.C.T. tissue cryosections were stained with antibodies against F4/80 (14-4801-85, 1:100, eBioscience), fibronectin (sc59826, 1:50, Santa Cruz), αSMA (CBL171, 1:500, EMD Millipore), Gr-1 (ab25377, 1:50, Abcam), CD31 (sc59906, 1:100, Santa Cruz), S100A4 (ab27957, 1:100, Abcam), epCAM (sc59906, 1:50, Santa Cruz), and TGFβ (ab66043, 1:100, Abcam). In some experiments, ECM evaluation was done by using antibodies directed to collagen I (ab6308, 1:100, Abcam), vitronectin (sc15332, 1:100, Santra Cruz), and tenascin C (ab6346, 1:100, Abcam). Secondary antibodies conjugated to Alexa Fluor 488, 555, or 594 were used (a11007, a11001, or a21424, respectively, 1:1000, Life Technologies). GFP and mCherry⁺ cells were detected by their intrinsic signal. Fluorescent images were obtained using a Nikon confocal microscope (Eclipse TE2000U) and analyzed using Nikon software (EZ-C1 3.6). FN and αSMA expression were quantified using ImageJ Software (NIH) by determining the ratio between the areas of FN and DAPI staining, expressed in arbitrary units (A.U).

Costa-Silva B., Aiello N.M., Ocean A.J., Singh S., Zhang H., Thakur B.K., Becker A., Hoshino A., Mark M.T., Molina H., Xiang J., Zhang T., Theilen T.M., García-Santos G., Williams C., Ararso Y., Huang Y., Rodrigues G., Shen T.L., Labori K.J., Lothe I.M., Kure E.H., Hernandez J., Doussot A., Ebbesen S.H., Grandgenett P.M., Hollingsworth M.A., Jain M., Mallya K., Batra S.K., Jarnagin W.R., Schwartz R.E., Matei I., Peinado H., Stanger B.Z., Bromberg J, & Lyden D.C. (2015). Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver. Nature cell biology, 17(6), 816-826.

+ Open protocol

+ Expand

Differentiation of Pluripotent Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Colonies growing on MEF were detached using Dispase/Collagenase IV (1 mg/ml each; both from Life Technologies) in DMEM/F12 and grown as a suspension culture on low adherent plates using hESC media without bFGF. After 1 week of suspension growth, cells were transferred to 12 or 24-well plates coated with 0.1% gelatin and grown in DMEM supplemented with 20% FBS, 0.1 mM nonessential amino acids, 2 mM GlutaMAX, 1% Pen/Strep and 64 μg/ml L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate. Embryoid bodies were grown for 1–2 weeks prior to fixation and immunofluorescence staining. Cultures were fixed and stained as described above using the following antibodies: AFP (1:200, SC-130302, Santa Cruz Biotech), FOXA2 (1:200, SC-6554, Santa Cruz Biotech), α-smooth muscle actin (1:1500, CBL171, EMD Millipore, Billerica, Massachussets) and MAP2 (1:200, sc-20172 and sc-74420, Santa Cruz Biotech).

Gallego Romero I., Pavlovic B.J., Hernando-Herraez I., Zhou X., Ward M.C., Banovich N.E., Kagan C.L., Burnett J.E., Huang C.H., Mitrano A., Chavarria C.I., Friedrich Ben-Nun I., Li Y., Sabatini K., Leonardo T.R., Parast M., Marques-Bonet T., Laurent L.C., Loring J.F, & Gilad Y. (2015). A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics. eLife, 4, e07103.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!