The ASPS-NE formulations were synthesized using the method of pseudoternary phase diagram (Yu et al., 2020 (link); Zhao et al., 2020 (link)). On the basis of the nanoemulsion regions in the phase diagram, ethanol was selected as the co-surfactant (Germany BASF Co., Ltd). Tween-80 and span-80 (Damao Chemical reagent factory, Shanghai, China) were selected as surfactants (Cao et al., 2019 (link); Wang et al., 2018 (link)). Liquid paraffin (Damao Chemical reagent factory, Tianjin, China), Isopropyl myristate (IPM, Lanxi Wumart Chemical Co., Ltd., Zhejiang, China) was selected as the oil phase, Acanthopanax senticosus polysaccharide (ASPS: purity =98%, average MW =30.51 kDa) was provided by Shanghai Yuanye Biotechnology Co., Ltd. First, k-Car-ASPS was dissolved in deionized water, and the resulting aqueous solution was then mixed with ethanol and liquid paraffin, followed by the addition of respective amounts of Tween-80, Span 80, and IPM. The clear and transparent nanoemulsion was obtained after magnetic stirring. The final NE formulation comprised 46.2% Tween-80, 15.4% Span 80, 7.6% ethanol, 15.4% liquid paraffin, 11.5% IPM, and 3.9% aqueous ASPS solution.
Span 80
Manufactured by Yuanye Bio-Technology
Sourced in China, United States
Span 80 is a non-ionic surfactant. It is a viscous, colorless to pale yellow liquid. Span 80 has an HLB value of 4.3 and is commonly used as an emulsifier, dispersant, and wetting agent in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Span 80, by Tianjin Damao (1 mentions)
Tween 80, by Yuanye Bio-Technology (1 mentions)
Hydrochloric acid (hcl), by Xilong (1 mentions)
Sodium hydroxide (naoh), by Xilong (1 mentions)
Soy protein isolate, by Yuanye Bio-Technology (1 mentions)
Maltodextrin, by Yuanye Bio-Technology (1 mentions)
Gelatin, by Yuanye Bio-Technology (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Milli q plus system, by Merck Group (1 mentions)
Protocatechuic aldehyde, by Yuanye Bio-Technology (1 mentions)
Mgcl2, by Tianjin Damao (1 mentions)
4 protocols using span 80
1
Pseudoternary Nanoemulsion Formulation of ASPS
2
Characterization of E. ulmoides Seed Oils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
E. ulmoides seed oils were purchased from Jiangxi Jingwang Natural Fragrance Co., Ltd., Xi'an Huageng Biotechnology Co., Ltd., Shaanxi Nexant Biotechnology Co., Ltd., and Jiangxi Hai Lin Fragrance Co., Ltd. Chitosan (CS) and gum arabic (GA) were acquired from Shanghai Macleans Biochemical Company. Hydroxyethyl cellulose (HEC) was obtained from Ashland Company. Span-80, maltodextrin (MD), sodium alginate (SA), soy protein isolate (SPI), and gelatin (Ge) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Transglutaminase was obtained from Taixing Dongsheng Food Co., Ltd.
Caprylic/capric triglyceride was purchased from Croda Cypro Chemical (Sichuan) Co., Ltd. Hydrochloric acid and sodium hydroxide (analytical grade) were purchased from Xilong Chemical Co., Ltd. Methanol (pure for mass spectrometry) was acquired from Fisher Scientific World, USA. N-hexane (analytically pure) was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. Finally, a 14% boron trifluoride methanol solution was purchased from Fuchen Chemical Reagent Co., Ltd.
Caprylic/capric triglyceride was purchased from Croda Cypro Chemical (Sichuan) Co., Ltd. Hydrochloric acid and sodium hydroxide (analytical grade) were purchased from Xilong Chemical Co., Ltd. Methanol (pure for mass spectrometry) was acquired from Fisher Scientific World, USA. N-hexane (analytically pure) was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. Finally, a 14% boron trifluoride methanol solution was purchased from Fuchen Chemical Reagent Co., Ltd.
3
Chitosan-Based Antioxidant Biomaterials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chitosan (medium viscosity: 200–400 mPa s, average molecular weight 50 000–150 000), carrageenan, 1,1-diphenyl-2-picrylhydrazyl (DPPH·), salicylic acid (≥98%), BSA (≥98%), UK (≥85%) ferrous chloride tetrahydrate (≥98%), and 2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxyl (PTIO·) were obtained from Aladdin Reagent Co., Ltd (Shanghai, China). Sodium tripolyphosphate (TPP, ≥98%) was ordered from Shanghai Acmec Biochemical Co., Ltd. Protocatechuic aldehyde (≥98%) and Span 80 were available from Shanghai Yuanye Bio-Technology Co., Ltd, paraffin and petroleum ether were purchased from Shanghai Titan Scientific Co., Ltd. Dulbecco's Modified Eagle Medium (DMEM), Phosphate Buffer Saline (PBS, 1×), fetal bovine serum (FBS), and trypsin–EDTA (0.25% trypsin and 0.913 mM EDTA tetrasodium) were purchased from Gibco (USA). Mouse fibroblast cells (L929) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cell Counting Kit-8 (CCK-8) was brought from Beyotime Biotechnology. A live & dead viability/cytotoxicity assay kit was obtained from YEASEN Inc (Shanghai, China). Sprague Dawley (SD) rats and balb/c mice were supplied by the affiliated hospital of Qingdao university laboratory animal center. Ultrapure water was supplied by Milli-Q Plus System (Millipore Corporation, United States). All reagents were used without further purification.
4
Synthesis of Graphene-Based Nanocomposites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Graphite was purchased from Sinopharm Chemical Reagent Co.,Ltd (Shanghai, China), Tween 80, NH3·H2O purchased from Shanghai Yien Chemical Technology Co., Ltd (Shanghai, China), cyclohexane were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China), Span 80 was purchased from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China), ethanol, CuSO4·5H2O, AgNO3, NaBH4, NaCl, FeCl3, glucose (Glu) and CaCl2 were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China), MgCl2 were purchased from Damao Chemical Reagent Factory (Tianjin, China), hydrogen peroxide(H2O2) was purchased from Jiangsu Qiangsheng functional Chemistry Co., Ltd (Jiangsu, China), Ltd (Beijing, China), glycine (Gly), lysine (Lys) were purchased from Nine-Dinn Chemistry Co., Ltd. (Shanghai, China), dopamine (DA), uric acid (UA), citric acid (CA), lactic acid (LA), arginine (Arg), valine(Val) were purchased from Shanghai McLean Biochemical Technology Co., Ltd (Shanghai, China), polyvinylpyrrolidone k30 (PVP k30) was purchased from Shanghai bide Pharmaceutical Technology Co., Ltd (Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!