Anti β3 defensin

Manufactured by Alpha Diagnostic

Sourced in United Kingdom

Anti-β3 defensin is a laboratory product used for research purposes. It is a protein that functions as an antimicrobial agent, playing a role in the immune system's defense against pathogens.

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Lab products found in correlation

Anti cd4, by Thermo Fisher Scientific (2 mentions) Anti cd3, by BD (2 mentions) Alexa 488 conjugated donkey anti rabbit antibody, by Thermo Fisher Scientific (2 mentions) Fv1000 confocal microscope, by Olympus (2 mentions) Anti γδ tcr, by BD (2 mentions) Anti vγ3 tcr, by BD (2 mentions) Anti ly6g gr 1, by Beckman Coulter (2 mentions) Anti camp, by Abcam (2 mentions) Tcs sp5 tandem scanner, by Olympus (2 mentions)

2 protocols using anti β3 defensin

Immunofluorescence Analysis of Skin Immune Cells

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Frozen sections were fixed with 4% paraformaldehyde/phosphate-buffered saline and stained with the following antibodies conjugated with Alexa 488 or FITC or Alexa 633: anti-CD3 (BD Pharmingen, San Diego, CA, clone 17A2), anti-CD4 (eBioscience, San Diego, CA, clone RM4-5), anti-γδ TCR (BD Pharmingen, clone GL3), anti-Vγ3 TCR (BD Pharmingen, clone 536), or anti-Ly6G/Gr-1 (Beckman Coulter, Miami, FL, clone RB6-8C5). Formalin-fixed paraffin sections were treated with citrate buffer for antigen retrieval and incubated with anti-Camp (Abcam, Cambridge, UK) or anti-β3 defensin (Alpha Diagnostic, San Antonio, TX) followed by Alexa 488-conjugated donkey anti-rabbit antibody (Life Technologies). DAPI or PI was used for nuclear staining. Images were acquired using a TCS SP5 Tandem Scanner or an FV1000 confocal microscope (Olympus, Tokyo, Japan). When quantifying cells that expressed a particular marker, at least six fields were analyzed per skin section.

Natsuga K., Cipolat S, & Watt F.M. (2016). Increased Bacterial Load and Expression of Antimicrobial Peptides in Skin of Barrier-Deficient Mice with Reduced Cancer Susceptibility. The Journal of Investigative Dermatology, 136(1), 99-106.

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Multicolor Immunofluorescence Staining of Skin Sections

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Frozen sections were fixed with 4% peraformaldehyde/PBS and stained with the following antibodies conjugated with Alexa 488, fluorescein isothiocyanate (FITC) or Alexa 633: anti-CD3 (BDPharmingen, clone 17.A2), anti-CD4 (eBioscience, clone RM4-5), anti-γδ TCR (BDPharmingen, clone GL3), anti-Vγ3 TCR (BDPharmingen, clone 536) or anti-Ly6G/Gr-1 (Beckman Coulter, clone RB6-8C5). Formalin-fixed paraffin sections were treated with citrate buffer for antigen retrieval and incubated with anti-Camp (Abcam) or anti-β3 defensin (Alpha Diagnostic) followed by Alexa 488-conjugated donkey anti-rabbit antibody (Life Technologies). DAPI or PI was used for nuclear staining. Images were acquired using a Leica TCS SP5 Tandem Scanner or an Olympus FV1000 confocal microscope. When quantifying cells that expressed a particular marker, at least 6 fields were analysed per skin section.

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