For immunofluorescence, tissue sections were incubated with the following primary antibodies: mouse anti-α-smooth muscle actin (α-SMA; 1:5000; Abcam, UK), rabbit anti-collagen Ⅳ (Col Ⅳ; 1:200; Abcam, UK), mouse anti-laminin (LN; 1:200; Abcam, UK). After being washed in PBS, sections were then incubated in 488- or 594-conjugated species-specific secondary antibody (1:1000; Chemicon, USA) for 2 h at room temperature. Slides were observed with an Olympus fluorescent microscope and images were captured using Olympus image viewer.
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Lab products found in correlation
Mouse anti α smooth muscle actin α sma, by Abcam (1 mentions)
Rabbit anti collagen 4 col 4, by Abcam (1 mentions)
Mouse anti laminin ln, by Abcam (1 mentions)
488 or 594 conjugated species specific secondary antibody, by Merck Group (1 mentions)
Fluorescent microscope, by Olympus (1 mentions)
Ix73 microscope, by Olympus (1 mentions)
2 protocols using image viewer
1
Immunofluorescence Staining of Tissue Sections
2
Quantifying Epithelial Thickness in Murine Skin
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The dorsal skin tissue samples from mice were fixed in formalin and dehydrated before being embedded in paraffin. Hematoxylin-eosin (H&E) or an anti-Ki67 antibody were utilized to stain paraffin sections taken from paraffin-embedded tissues after they had been deparaffinized by xylene and rehydrated through a graduated series of ethanol. Olympus Image Viewer was used to quantify the epithelial thickness after images were taken with an Olympus microscope (IX73).
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