Essential amino acids

All chemicals were purchased from NeoFroxx unless otherwise stated. To maintain cells outside of the incubation chamber, a cell storage buffer containing 2 mM CaCl₂, 5 mM KCl, 138 mM NaCl, 1 mM MgCl₂, 1 mM HEPES (Pan-Biotech, Aidenbach, Germany), 0.44 mM KH₂PO₄, 2.6 mM NaHCO₃, 0.34 mM NaH₂PO₄, 10 mM D-Glucose, 0.1% MEM Vitamins (Pan-Biotech, Aidenbach, Germany), 0.2% essential amino acids (Pan-Biotech, Aidenbach, Germany), 100 µg/mL Penicillin (Pan-Biotech, Aidenbach, Germany), and 100 U/mL Streptomycin (Pan-Biotech, Aidenbach, Germany) was used. The pH was adjusted to 7.43 using 1 M NaOH. The cell storage buffer was sterilized using a 0.45 µm medium filter (Isolab, Germany). For live-cell imaging experiments, a HEPES-buffered solution was used consisting of 2 mM CaCl₂, 5 mM KCl, 138 mM NaCl, 1 mM MgCl₂, 10 mM HEPES, 10 mM D-Glucose, and pH was adjusted to 7.43 using 1 M NaOH.

If not otherwise stated, all chemicals were purchased from NeoFroxx. To maintain cells outside of the incubation chamber, a cell storage buffer containing 2 mM CaCl₂, 5 mM KCl, 138 mM NaCl, 1 mM MgCl₂, 1 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Pan-Biotech, Aidenbach, Germany), 0.44 mM KH₂PO₄, 2.6 mM NaHCO₃, 0.34 mM NaH₂PO₄, 10 mM D-Glucose, 0.1% minimum essential medium (MEM) Vitamins (Pan-Biotech, Aidenbach, Germany), 0.2% essential amino acids (Pan-Biotech, Aidenbach, Germany), 100 µg/mL Penicillin (Pan-Biotech, Aidenbach, Germany) and 100 U/mL Streptomycin (Pan-Biotech, Aidenbach, Germany) was used. The pH was adjusted to 7.43 using 1 M NaOH. The cell storage buffer was sterilized using a 0.45 µm medium filter (Isolab, Eschau, Germany). For live-cell imaging experiments, a HEPES-buffered solution was used, consisting of 2 mM CaCl₂, 5 mM KCl, 138 mM NaCl, 1 mM MgCl₂, 10 mM HEPES, 10 mM D-Glucose, and pH was adjusted to 7.43 using 1 M NaOH.