M smegmatis

Manufactured by American Type Culture Collection

Sourced in United States

M. smegmatis is a strain of non-pathogenic mycobacteria commonly used in laboratory research. It is a fast-growing, saprophytic species that serves as a model organism for the study of mycobacterial biology and physiology.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

M. smegmatis mc2 155 (8 citations)

6 protocols using m smegmatis

Mycobacterial Cell Culture Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

MTB H37Rv (ATCC 27294), MTB H37Ra (ATCC 25177), M. bovis BCG (ATCC 19274) and M. smegmatis (ATCC 19420) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). All mid-log mycobacteria (OD₆₀₀ = 0.5–0.6) used in this study were prepared as described previously^{24 (link)}.

Koh H.J., Kim Y.R., Kim J.S., Yun J.S., Kim S., Kim S.Y., Jang K, & Yang C.S. (2018). CD82 hypomethylation is essential for tuberculosis pathogenesis via regulation of RUNX1-Rab5/22. Experimental & Molecular Medicine, 50(5), 62.

+ Open protocol

+ Expand

Preparation of Mycobacterial Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lyophilized M.tb H₃₇R_v (ATCC# 27294), M.tb H₃₇R_a (ATCC# 25177), M. bovis BCG (ATCC# 35734), and M. smegmatis (ATCC# 700084) were obtained from the American Type Culture Collection (ATCC, Manassas, VA). GFP-M.tb Erdman was a kind gift from Dr. Marcus Horwitz, UCLA, CA). Clinical isolates CDC1551 and HN878 were from BEI Resources (Manassas, VA). M.tb H₃₇R_v-mCherry was kindly provided by Dr. Sarah Fortune (Harvard University). Single cell suspensions of bacteria were prepared as previously described [75 (link),76 ]. The bacteria concentration and degree of clumping (<10%) were determined with a Petroff-Hausser Chamber. This method results in approximately 90% viable bacteria, as determined by CFU assay.

Leopold Wager C.M., Bonifacio J.R., Simper J., Naoun A.A., Arnett E, & Schlesinger L.S. (2023). Activation of transcription factor CREB in human macrophages by Mycobacterium tuberculosis promotes bacterial survival, reduces NF-kB nuclear transit and limits phagolysosome fusion by reduced necroptotic signaling. PLOS Pathogens, 19(3), e1011297.

+ Open protocol

+ Expand

Cultivation and Enumeration of Mycobacterial Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mycobacterium tuberculosis H37Rv (M. tuberculosis), M. bovis BCG and M. smegmatis (ATCC, Manassas, VA) were cultured in Middlebrook 7H9 broth medium (Difco Laboratories, Detroit, MI). After incubating M. tuberculosis and M. bovis BCG for 21 days and M. smegmatis for 3 days at 37 °C, the mycobacterial stock solution was harvested, aliquoted and stored at −80 °C until use. Colony-forming units (CFUs) were enumerated after the disruption of mycobacterial clumps [18 (link)].

Juárez E., Ruiz A., Cortez O., Sada E, & Torres M. (2018). Antimicrobial and immunomodulatory activity induced by loperamide in mycobacterial infections. International Immunopharmacology, 65, 29-36.

+ Open protocol

+ Expand

Cloning and Expression of M. tuberculosis rv3619c

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA isolated from M. tuberculosis H37Rv (obtained from the American Type Culture Collection, Rockville, MD, USA) served as the source for the amplification and subsequent cloning of the rv3619c gene, as previously described [9 (link)]. In brief, DNA corresponding to the rv3619c gene was amplified by PCR using genomic DNA isolated from M. tuberculosis and gene-specific primers (ThermoFisher Scientific, Ulm, Germany) (online suppl. Table S1; see www.karger.com/doi/10.1159/000525136 for all online suppl. material) and then ligated to appropriated cloning and expression vectors i.e., expression vector pGES-TH1 [9 (link)], shuttle vector pDE22 [12 (link)], and DNA plasmid vector pUMVC6 [12 (link)] (Aldevron, Fargo, ND, USA), as described previously [9 (link), 11 (link), 12 (link)] for expression in E. coli BL-21 (Novagen, Madison, WI, USA), M. smegmatis (ATCC 700084/mc(2)155, ATCC, Manassas, VA, USA), and DNA plasmid vector pUMVC6, respectively.

Safar H.A., Mustafa A.S., Amoudy H.A, & El-Hashim A. (2022). The Effect of Delivery Systems on the Induction of T Helper 1 Cell Response to an ESAT6-Like Protein Rv3619c and Identification of Its Immunodominant Peptides. Medical Principles and Practice, 31(4), 359-367.

+ Open protocol

+ Expand

Antimicrobial Resistance Testing Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

MRSA (ATCC 700699, USA300), S. epidermidis (ATCC 14990), Enterococcus faecalis (ATCC 29212), S. pneumoniae (ATCC 49619), E. coli (ATCC 25922, ATCC 35218), P. aeruginosa (ATCC 27853), K. pneumoniae (MDR, ATCC 75297), A. baumannii (ATCC 19606), M. smegmatis (ATCC 70084), HUVECs, and CC-HHM-2 were obtained from the ATCC (Manassas, USA). S. aureus (ATCC 29213), enterohemorrhagic E. coli (0157:H7), S. typhimurium (SL 1344), and M. tuberculosis (H37Rv) were obtained from the Chinese National Center for Surveillance of Antimicrobial Resistance (Beijing, China). The clinical MRSA strains (XJ 75302, XJ 141240, and XJ 140718), E. coli (XJ 74283), P. aeruginosa (XJ 75315), and A. baumannii (XJ 17014279 and XJ 17014346) were obtained from Xijing Hospital (Xi’an, China).

Qu D., Hou Z., Li J., Luo L., Su S., Ye Z., Bai Y., Zhang X., Chen G., Li Z., Wang Y., Xue X., Luo X, & Li M. (2020). A new coumarin compound DCH combats methicillin-resistant Staphylococcus aureus biofilm by targeting arginine repressor. Science Advances, 6(30), eaay9597.

+ Open protocol

+ Expand

Comprehensive Evaluation of NTM Isolates

Check if the same lab product or an alternative is used in the 5 most similar protocols

Twelve NTM reference strains and 227 NTM clinical isolates (194 M. abscessus, 14 M. avium, 15 M. intracellulare, and 4 M. fortuitum) were evaluated. The reference strains, M. abscessus subsp. abscessus (ATCC 19977), M. smegmatis (ATCC 19420), M. avium (ATCC 25291), M. intracellulare (ATCC 13950), M. kansasii (ATCC 12478), M. fortuitum (ATCC 6841), M. gordonae (ATCC 14470), M. scrofulaceum (ATCC 19981), M. szulgai (ATCC 35799), M. xenopi (ATCC 19250), and M. peregrinum (ATCC 700686), were purchased from the American Type Culture Collection (VA, USA). M. abscessus subsp. massiliense (CIP 108297) was purchased from the Biological Resource Center of Institute Pasteur (Paris, France). The clinical isolates were obtained from the Shanghai Pulmonary Hospital from sputum and bronchoalveolar lavage fluid specimens of patients with NTM lung infections. All M. abscessus isolates were sequenced; the full genome sequence of each isolate was published and is available at DDBJ/ENA/GenBank (BioProject PRJNA488058, PRJNA448987, and PRJNA398137). All strains were grown at 37°C in Middlebrook 7H9 broth supplemented with 10% OADC or on Middlebrook 7H10 plates supplemented with 10% OADC.

Wu W., He S., Li A., Guo Q., Tan Z., Liu S., Wang X., Zhang Z., Li B, & Chu H. (2022). A Novel Leucyl-tRNA Synthetase Inhibitor, MRX-6038, Expresses Anti-Mycobacterium abscessus Activity In Vitro and In Vivo. Antimicrobial Agents and Chemotherapy, 66(9), e00601-22.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!