Frozen sections (4-μm thick) of liver tissue were prepared for immunofluorescence staining. In brief, sections were stained by overnight incubation with primary antibodies, such as anti-KIAA1199 (Proteintech, 1:200), anti-CK18 (Abcam, 1:200), and anti-phalloidin (Abcam, 1:200). Alexa 488-conjugated goat anti-rabbit (A11008, Thermo Fisher; 1:1000) or Alexa 568-conjugated goat anti-mouse (A-21043, Invitrogen; 1:1000) secondary antibodies were used. After three washes with PBST, the slides were incubated with DAPI (Beyotime, 1:2000) for 5 min. In addition, LC3 lentivirus was transfected into CCA cells according to the manufacturer’s recommendations. Images were acquired with a fluorescence microscope (LSM 780, Carl Zeiss).
Anti kiaa1199
Manufactured by Proteintech
Sourced in United Kingdom
Anti-KIAA1199 is a laboratory reagent used to detect the presence of the KIAA1199 protein in biological samples. It is a specific antibody that binds to the KIAA1199 protein, allowing researchers to identify and quantify its expression in cells or tissues.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 780, by Zeiss (2 mentions)
A 21043, by Thermo Fisher Scientific (1 mentions)
Anti ck18, by Abcam (1 mentions)
A11008, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Anti hif 1α antibody, by Abcam (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)
Ecl reagent, by Thermo Fisher Scientific (1 mentions)
Cell lysis buffer, by Beyotime (1 mentions)
Anti β actin, by Abcam (1 mentions)
Polyvinylidene difluoride membrane, by Roche (1 mentions)
Anti ctgf, by Santa Cruz Biotechnology (1 mentions)
Anti caldesmon, by Merck Group (1 mentions)
Anti sox2, by Santa Cruz Biotechnology (1 mentions)
3 protocols using anti kiaa1199
1
Immunofluorescence Staining of Liver Tissue
2
Western Blot Analysis of KIAA1199 and HIF-1α
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Cells or tissues were lysed with the cell lysis buffer (Beyotime, Shanghai, China). Whole cell or tissue extracts were resolved by 10% SDS-PAGE and electrophoretically transferred to polyvinylidene difluoride membranes (Roche Diagnostics, Mannheim, Germany). The membranes were blocked, and then incubated with anti-KIAA1199 (Cat No: 21129-1-AP, ProteinTech Group, Chicago, IL), anti-β-actin (Cat No: ab8226, Abcam, Cambridge, MA) or anti-HIF-1α antibodies (Cat No: ab1, Abcam, Cambridge, MA) at 4°C overnight, followed by an incubation with the appropriate horseradish peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch, PA). The chemiluminescence reaction was performed using ECL reagent (Thermo Scientific, IL). The specificity of KIAA1199 and HIF1α antibodies by RNA interference were confirmed by RNA interference (Supplementary Fig. 1).
3
Immunohistochemical Analyses of Cell Markers
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The primary antibodies used in this study are as follows: anti-KIAA1199 (rabbit immunoglobulin G (IgG), Proteintech, Manchester, UK, 21129-1-AP, 1 : 50, rabbit IgG, Cosmo Bio, Tokyo, Japan, CNP-IP-208, 1 : 50), anti-SOX2 (goat IgG, Santa Cruz Biotechnology, Dallas, TX, US, sc17320, 1 : 100), anti-CTGF (goat IgG, Santa Cruz Biotechnology, sc14939, 1 : 100), anti-CALDESMON (Sigma-Aldrich, C0297, 1 : 100), anti-NKCC1 (goat IgG, Santa Cruz Biotechnology, sc21545, 1 : 300), anti-MYOSIN7a (mouse IgG, DSHB, Iowa City, IA, US, 138-1-s, 1 : 30), and anti-β-III TUBULIN (mouse IgG, Sigma-Aldrich, T8660, 1 : 250).
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