Alexa 488 donkey anti mouse

Manufactured by Merck Group

Alexa Fluor 488 Donkey Anti-Mouse is a secondary antibody that is conjugated with the Alexa Fluor 488 fluorescent dye. It is designed to detect and visualize mouse primary antibodies in various immunodetection applications, such as Western blotting, immunohistochemistry, and flow cytometry.

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Lab products found in correlation

Mouse monoclonal anti gfp, by Merck Group (2 mentions) Chicken anti gfp, by Aves Labs (2 mentions) Donkey anti chicken alexa 488, by Thermo Fisher Scientific (2 mentions) Mouse anti huc d, by Thermo Fisher Scientific (2 mentions) Rabbit anti dsred, by Takara Bio (2 mentions) Goat anti rabbit alexa 555, by Thermo Fisher Scientific (2 mentions) Cy 3 anti rabbit, by Merck Group (2 mentions) Alexa 633 donkey anti rabbit, by Thermo Fisher Scientific (2 mentions) Mouse anti acetylated tubulin, by Merck Group (2 mentions)

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Alexa 488 (40 citations)

2 protocols using alexa 488 donkey anti mouse

Comprehensive Immunohistochemistry Labeling Protocol

Cited 1 time

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Immunohistochemistry was performed as previously described (Bonkowsky et al., 2008 (link)). Antibodies used were: mouse anti-acetylated tubulin 1:250 (Sigma), mouse monoclonal anti-GFP 1:250 (Millipore), chicken anti-GFP 1:1000 (Aves Labs), mouse anti-HuC/D 1:400 (ThermoFisher), rabbit anti-dsRed 1:250 (Clontech), Cy-3 anti-rabbit 1:400 (Millipore), Alexa 488 donkey anti-mouse 1:400, Alexa 633 donkey anti-rabbit, Alexa 488 donkey anti-chicken 1:400, Alexa 555 rabbit anti-goat 1:400 (ThermoFisher), and 4',6-diamidino-2-phenylindole (DAPI).

Keefe M.D., Soderholm H.E., Shih H.Y., Stevenson T.J., Glaittli K.A., Bowles D.M., Scholl E., Colby S., Merchant S., Hsu E.W, & Bonkowsky J.L. (2020). Vanishing white matter disease expression of truncated EIF2B5 activates induced stress response. eLife, 9, e56319.

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Multicolor Immunohistochemistry Protocol

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Immunohistochemistry was performed as previously described (Bonkowsky et al., 2008) .
Antibodies used were: mouse anti-acetylated tubulin 1:250 (Sigma), mouse monoclonal anti-GFP 1:250 (Millipore), chicken anti-GFP 1:1000 (Aves Labs), mouse anti-HuC/D 1:400 (ThermoFisher), rabbit anti-dsRed 1:250 (Clontech), Cy-3 anti-rabbit 1:400 (Millipore), Alexa 488 donkey anti-mouse 1:400, Alexa 633 donkey anti-rabbit, Alexa 488 donkey anti-chicken 1:400, Alexa 555 rabbit anti-goat 1:400 (ThermoFisher), and 4',6-diamidino-2-phenylindole (DAPI).

Keefe M.D., Soderholm H.E., Shih H., Stevenson T.J., Glaittli K.A., Bowles D.M., Schöll E., Colby S., Merchant S., Hsu E.W., & Bonkowsky J.L. (2020). Vanishing White Matter Disease Expression of Truncated EIF2B5 Activates Induced Stress Response.

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