Dako envision plus kit

Manufactured by Agilent Technologies

Sourced in United States

The Dako Envision Plus Kit is a reagent system used for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. It is designed to detect primary antibodies binding to target antigens in the tissue samples.

Automatically generated - may contain errors

Lab products found in correlation

Automated immunostainer, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Dako Envision Flex Plus Mouse Link Kit DAKO Envision kit Envision Plus kit Envision Plus Dako kits Envision kit

2 protocols using dako envision plus kit

Immunohistochemical Analysis of CD81 and CD44

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse xenograft lung tissues or patient primary tumors were paraffin-embedded and sectioned by routine techniques. Heat-induced antigen retrieval was achieved using Decloaker solution for 15–20 min (Biocare Medical, RD913L). Tissue sections were blocked with TBS/10% NGS, then incubated with CD81 (Cat # HPA007234 Millipore Sigma, St. Louis, MO, USA; RRID:AB_1846333) or CD44 (Cat# MA513890, Thermo Fisher Scientific, Waltham, MA, USA; RRID:AB_10986810) primary antibody overnight, followed by Dako envision plus kit and DAB staining (Dako K4010). All samples were counterstained with hematoxylin.

Ramos E.K., Tsai C.F., Jia Y., Cao Y., Manu M., Taftaf R., Hoffmann A.D., El-Shennawy L., Gritsenko M.A., Adorno-Cruz V., Schuster E.J., Scholten D., Patel D., Liu X., Patel P., Wray B., Zhang Y., Zhang S., Moore R.J., Mathews J.V., Schipma M.J., Liu T., Tokars V.L., Cristofanilli M., Shi T., Shen Y., Dashzeveg N.K, & Liu H. (2022). Machine learning-assisted elucidation of CD81–CD44 interactions in promoting cancer stemness and extracellular vesicle integrity. eLife, 11, e82669.

+ Open protocol

+ Expand

Immunohistochemical Analysis of PGP 9.5 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemical staining with antibodies against PGP 9.5 (Novacastra Laboratories, Newcastle upon Tyne, UK) on TMA slides was conducted by using an automated immunostainer (DAKO, Carpinteria, CA). Briefly, sections were deparaffinized in xylene, rehydrated through decreasing concentrations of alcohol ending in phosphate-buffered saline, subjected to steam heat in 10 mmol/L citrate buffer (pH 6.0) for 40 minutes in a vegetable steamer, then allowed to cool off at room temperature for an additional 10 minutes. After endogenous peroxidase activity was quenched in 3% hydrogen peroxide solution in distilled water, sections were incubated with rabbit polyclonal antibody against PGP 9.5 (1:40, overnight at 48°C; cat no. 9462). Sections were washed and the bound antibody was detected by using a DAKO Envision Plus kit (DAKO) with diaminobenzidine as chromogen. Finally, sections were counterstained with hematoxylin and eosin, dehydrated, and mounted. Negative controls were sections immunostained as above, but normal rabbit serum was used instead of primary antibody.

Olar A., He D., Florentin D., Ding Y, & Ayala G. (2014). Biologic Correlates and Significance of Axonogenesis in Prostate Cancer. Human pathology, 45(7), 1358-1364.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!