tin chloride, dopamine, acridine orange,
and tris hydrochloride buffer salt were obtained from Sigma-Aldrich.
The sensing-related DNA samples were procured from Integrated DNA
Technologies, and the DNA sequences used in this paper are listed
as follows.
DNA1: 3′-CTGGAAGGAAAGGAAGGCCTACTTCGACTACAGTCTCAG-5′
DNA2: 3′-TACGGATCGATGATCGGATCATCGCAGTCC-5′
DNA3: 3′-TAGGGTTGGGTATGGGAAGGGTCCCTCCAACCTATACCTTCCACCCA-5′
DNA4: 3′-TTAGGATCTGGTTACTCCGTCTACCT-5′
DNA5:
3′-TCGACGTAGCTATCGTACGCCTACCTCTA-5′
The stock
solution of aptamer was prepared by dissolving 50 mM
Tris-HCl buffer and stored at 4 °C. The nitrate salts of lead,
silver, mercury, calcium, and nickel, and the chloride salts of cobalt,
copper, barium, ferric, magnesium, ferrous, manganese, and other standard
chemicals were purchased at the highest purity levels from SRL and
Sigma-Aldrich (India). Throughout the experiment, deionized (DI) water
was used.