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Tris hydrochloride buffer

Manufactured by Merck Group
Sourced in Germany

Tris hydrochloride buffer is a commonly used buffer solution that maintains a stable pH environment for various biochemical and molecular biology applications. It is composed of tris(hydroxymethyl)aminomethane (Tris) and hydrochloric acid (HCl), which together create a buffering system that helps maintain a desired pH level, typically in the range of 7.0 to 9.0.

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5 protocols using tris hydrochloride buffer

1

Fluorescent DNA Sensor Development

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Calcium nitrate,
tin chloride, dopamine, acridine orange, l-cysteine, EDTA,
and tris hydrochloride buffer salt were obtained from Sigma-Aldrich.
The sensing-related DNA samples were procured from Integrated DNA
Technologies, and the DNA sequences used in this paper are listed
as follows.
DNA1: 3′-CTGGAAGGAAAGGAAGGCCTACTTCGACTACAGTCTCAG-5′
DNA2: 3′-TACGGATCGATGATCGGATCATCGCAGTCC-5′
DNA3: 3′-TAGGGTTGGGTATGGGAAGGGTCCCTCCAACCTATACCTTCCACCCA-5′
DNA4: 3′-TTAGGATCTGGTTACTCCGTCTACCT-5′
DNA5:
3′-TCGACGTAGCTATCGTACGCCTACCTCTA-5′
The stock
solution of aptamer was prepared by dissolving 50 mM
Tris-HCl buffer and stored at 4 °C. The nitrate salts of lead,
silver, mercury, calcium, and nickel, and the chloride salts of cobalt,
copper, barium, ferric, magnesium, ferrous, manganese, and other standard
chemicals were purchased at the highest purity levels from SRL and
Sigma-Aldrich (India). Throughout the experiment, deionized (DI) water
was used.
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2

Antibacterial PES Membrane Fabrication

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Dopamine hydrochloride
(Scheme S1a), tris hydrochloride buffer,
and sodium hydroxide were purchased from Sigma-Aldrich. Cetyltrimethylammonium
bromide (CTAB, MW: 365 Da) (Scheme S1b)
was supplied by Alfa Aesar and used as an antibacterial agent. Phosphate-buffered
saline (PBS), sodium chloride (NaCl), and isopropyl alcohol (IPA)
were obtained from Sigma-Aldrich. sodium hydroxide (NaOH) and hydrochloric
acid (HCl) with 37% purity used for pH adjustments were purchased
from Sigma-Aldrich and Merck, respectively. Gram-negative (E. coli, ATCC 25922) and Gram-positive (S. aureus, RSKK 1009) bacteria were used in anti-biofouling
tests. The commercial PES UF support membranes (NADIR PM UP150) with
a reported nominal molecular-weight limit of 150 kDa were supplied
by MicroDyn Nadir. Deionized water with a conductivity of 0.05 μS/cm
was used for the experiments. All chemicals were used as received
without further purification.
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3

Fabrication of Fluoropolymer-based Surfaces

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Amorphous fluoroplastics precursor (6%; Chemours, Teflon AF 1601), amorphous FP CYTOP (AGC Chemicals, CTL-809M), fluorinated solvent solution (AGC Chemicals, SOLV180), APTS (95%; Dieckmann), deionized water [resistivity of 18.3 megohm·cm, produced by a deionized water system (DINEC, Hong Kong)], hydrogen peroxide solution (30%; Sigma-Aldrich), sulfuric acid solution (≥99%; Sigma-Aldrich), BSA (≥99%; Sigma-Aldrich), tris hydrochloride buffer (>99%; Sigma-Aldrich), sodium hydroxide (≥98%; Sigma-Aldrich), hydrochloric acid (37% in water), ferric chloride (≥99%; Sigma-Aldrich), ethanol (≥99%; Sigma-Aldrich), glycerin (≥99%; Sigma-Aldrich), glycol (≥99%; Sigma-Aldrich), sodium chloride (≥99%; Sigma-Aldrich), and sodium alga acid (low-viscosity model; Sigma-Aldrich) were used without further purification. Ultra-Ever Dry was purchased from Ultra Tech International Inc. Copper was purchased from the common hardware stores in Hong Kong.
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4

Phytochemical Analysis of Sophora Flavescens Roots

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SF roots, grown in Dak Nong, Vietnam, from February 2017 to February 2019, were collected and analytically identified. The standard compounds matrine, oxymatrine, and sophoridine were imported from Chengdu Biopurify Phytochemicals Ltd., Sichuan, China. Ethanol (EtOH), methanol (MeOH), acetic acid (glacial) (AcOH), acetonitrile (ACN), chloroform, DMSO, and Tris hydrochloride buffer were purchased from Merck, Darmstadt, Germany. Acetylcholinesterase (AChE), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich, Burlington, MA, USA. Cells culture materials such as the DMEM medium, fetal bovine serum (PBS), L-glutamine, Penicillin–Streptomycin (PenStrep), and trypsin-EDTA were supplied by Gibco, Grand Island, NY, USA.
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5

Dissection and Histology of Temporal Bones

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Six bullae (middle ears) were completely dissected from the temporal bone to locate the mucosal membrane in each bulla and to compare locations. It was possible to harvest them easily and completely. Subsequently, temporal bones were fixed overnight at 4℃ in a buffered solution of paraformaldehyde (Merck ultra pure 4005, Germany). Thereafter, temporal bones were decalcified for 5 days at 4℃ in a Tris-hydrochloride buffer (0.1 mol/L, pH 7.4) (Merck), ethylenediamine tetra-acetic acid (EDTA) (10% weight/volume) (Merck), and polyvinylpyrrolidone (7.5%) (Serva, Heidelberg, Germany).3 (link) Decalcified bones were rinsed in the same buffer, except without EDTA (4 hours, 4℃). Each specimen was then embedded in paraffin and cut according to the standard histologic technique and stained with hematoxylin and eosin.
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