Anti trail r2 2019

Western blot analysis was performed as described previously [48 (link)]. For whole-cell lysates, cells were lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (both from Roche, Mannheim, Germany). One representative experiment out of at least three performed is shown. Primary antibodies were purchased from Cell Signaling, Frankfurt, Germany [anti-MDM2 (86934; used for all western blots); anti-p21 (2947); anti-PML (33156; used for all western blots)], Santa Cruz Biotechnology [anti-p53 (DO-1, used for all western blots except for blots shown in Figs. 5B, 6B); anti-p53 (FL393, for Figs. 5B, 6B), BD Transduction Laboratories, Heidelberg, Germany [anti-Bax (610982)], Merck Millipore, Darmstadt, Germany [anti-DR4 (AB16955)], Epitomics, California, USA [α-Tubulin (1878-1)], ProScience Incorporated, Poway, USA [anti-TRAIL-R2 (2019)] and from Sigma-Aldrich [anti-β-Actin (A5441)]. Secondary antibodies were purchased from Cell Signaling [anti-rabbit-HRP (7074) and anti-mouse-HRP (7076)].

Cells lysates were supplemented with Complete Protease Inhibitor Cocktail and PhosphoStop (both from Roche, Mannheim, Germany). Western blot analyses were performed, as described previously [18 (link)]. Antibodies used for western blots were purchased from: Cell Signaling, Frankfurt, Germany (anti-TRAIL-R2 (3696), anti-Lamin A/C (2032), anti-Histone H3 (9715)), Epitomics, Burlingame CA, USA (anti-beta Tubulin (1879), anti-alpha Tubulin (1878)), Merck Millipore, Darmstadt, Germany (anti-TRAIL-R1 (AB16955)), ProScience Incorporated, Poway CA, USA (anti-TRAIL-R2 (2019)), Sigma-Aldrich, Munich, Germany (anti-TRAIL (T9191), anti-β-actin (A5441)), BD Bioscience, Heidelberg, Germany (anti-CRM-1 (611833)), Thermo Scientific, Waltham, USA, (anti-POLR2A (MA1-46093)).

Preparation of whole cell lysates, electrophoresis and Western blotting were conducted as described [36 (link)]. Primary antibodies were purchased from: ProScience Incoporated, USA (anti-TRAIL-R2 (2019)), Sigma-Aldrich (anti-β-actin (A5441)).