Roswell Park Memorial Institute (RPMI)-1640 and Dulbecco’s Modified Eagle Medium (DMEM) medium, glucose, L-glutamine, sodium pyruvate, and antibiotic-antimycotic solution (amphotericin B, penicillin, streptomycin) were purchased from Life Technologies. Fetal bovine serum (FBS) was purchased from Biological Industries. Gemcitabine, dimethyl sulfoxide (DMSO), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma. The 2X KAPA SYBR FAST qPCR Master Mix was purchased from Kapa Biosystems. The iScript cDNA Synthesis Kit was purchased from Bio-Rad.
Dulbecco s modified eagle medium dmem medium
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium formulation that supports the growth and maintenance of various cell types in cell culture applications. It provides a balanced salt solution, essential amino acids, vitamins, and other nutrients required for cell proliferation and survival.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (10 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Sartorius (2 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
Iscript cdna synthesis kit, by Bio-Rad (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Amphotericin b, by Thermo Fisher Scientific (1 mentions)
Gemcitabine, by Merck Group (1 mentions)
Kapa sybr fast qpcr master mix, by Roche (1 mentions)
Nec 1, by Merck Group (1 mentions)
Fer 1, by Merck Group (1 mentions)
Hepes, by Harvard Bioscience (1 mentions)
Erastin, by Merck Group (1 mentions)
24 protocols using dulbecco s modified eagle medium dmem medium
1
Cell Culture Reagents and Assays
2
Cell Line Culture and Compound Treatments
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ALL cell lines were obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 or Dulbecco's Modified Eagle Medium (DMEM) medium (Life Technologies, Inc., Eggenstein, Germany), supplemented with 10% FCS (fetal calf serum) (Biochrom, Berlin, Germany), 1% penicillin/streptomycin (Invitrogen) and 25 mM HEPES (Biochrom). The bivalent Smac mimetic BV6, which antagonizes XIAP, cIAP1 and cIAP2 [14 (link)], was kindly provided by Genentech Inc. (South San Francisco, CA, USA). Erastin, Fer-1, DFO and α-Toc were purchased from Sigma-Aldrich (Taufkirchen, Germany), zVAD.fmk from Bachem (Heidelberg, Germany), Nec-1 from Merck (Darmstadt, Germany) and recombinant human TNFα from Biochrom (Berlin, Germany). RSL3 was kindly provided by B. Stockwell (Columbia University, New York, NY, USA) or purchased from InterBIOScreen Ltd. (Moscow, Russia). All chemicals were purchased by Sigma-Aldrich or Carl Roth (Karlsruhe, Germany) unless indicated otherwise.
3
MDA-MB-231 Spike-in Experiment
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The human breast cancer cell line MDA-MB-231 cell line was purchased from ATCC and cells were maintained in 5% CO2 at 37 °C incubation in complete Dulbecco’s Modified Eagle Medium (DMEM) medium (Life Technologies, Carlsbad, CA, USA). Cells were washed twice with 1xPBS (Life Technologies, Carlsbad CA, USA). At 75–80% confluency cells were detached using trypsin-EDTA (0.05%) (Life Technologies, CA, Carlsbad, USA). For the spike-in experiment, MDA-MB-231 cells (n = 50) were spiked into 7.5 mL healthy donor whole blood in a Cell-Free DNA BCT tube (Streck, La Vista, NE, USA). MDA-MB-231 bulk cancer cells were used as a positive control. We used WBCs isolated from peripheral blood not processed on the Parsortix as a negative control (no spiked cells). The spike-in experiment was done in duplicate.
4
Synthesis and Characterization of Biodegradable Polymers
5
293T Cells Transfected with AMPD1
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293T-cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)medium (Invitrogen, CA, United States) with the addition of 10% fetal calf serum and 500 µ/ml penicillin, 500U/ml streptomycin. The construction of expression plasmid pEGFP-C3-AMPD1 was then transfected into the 293T-cells after the second day of cell passage. Briefly, the transient transfection of 293T-cells was performed using Lipofectamine (Invitrogen, CA, United States) according to the manufacturer’s instructions. Approximately 1.0 µg of cDNA per 150,000 cells was used in 6-well plates. Then cell DNA was stained with hoechst33342 for 30 min after transfection for 48 h, then cell fluorescence was imaged using Zeiss LSM510 confocal microscope (Carl Zeiss AG, Oberkochen, Germany).
6
Regulation of Angiogenesis by miR-424 in Endothelial Cells
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Dulbecco's modified eagle medium (DMEM) medium, Roswell Park Memorial Institute (RPMI) 1640 medium, and LipofectamineTM 2000 were purchased from Invitrogen, USA; fetal bovine serum, penicillin, streptomyces, trypsin, and Radio Immunoprecipitation Assay (RIPA) Lysis Buffer were purchased from Beijing Solabao Technology Co., Ltd. (Beijing, China); propranolol hydrochloride was purchased from Shanghai Latin Life Technology Co., Ltd. (Shanghai, China); TransZol Up Plus RNA Kit was purchased from Beijing Quanshijin Biotechnology Co., Ltd. (Beijing, China); PrimeScriptTM RT Master Mix was purchased from Tiangen Biochemical Technology (Beijing, China); Negative Control (NC) inhibitor, NC mimic, miR-424 inhibitor, and miR-424 mimic was purchased from Shanghai Abbots Biotechnology Co., Ltd. (Shanghai, China); primers were synthesized by Guangzhou Kinco Biotechnology Co., Ltd. (Guangzhou, China); TaqMan MicroRNA was purchased from Thermofisher, USA; specific primary antibodies [vascular endothelial growth factor-A (VEGFA) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)] and secondary antibodies immunoglobulin-G (IgG) were purchased from Shanghai Abkang Trading Co., Ltd. (Shanghai, China); crystal violet, 4% paraformaldehyde, Cell counting kit-8 (CCK-8) Kit, and Annexin V-FITC/PI Kit were purchased from Shanghai Biyuntian Biotechnology Co., Ltd.(Shanghai, China).
7
Isolation and Culture of Neonatal Rat Cardiomyocytes
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The neonatal rat cardiomyocytes are isolated from 1 to 3-day old neonatal Sprague Dawley rats using a previously described protocol [30 (link)]. The design and protocol for animal studies were approved by the Animal Study Ethic Community of Jiamusi University (#JMSU2017011). Rats were anesthetized with isoflurane and were sacrificed with the cervical dislocation method. Briefly, the left ventricle cardiac tissues were cut and digested with collagenase (Sigma) and trypsin (Gibco) to prepare single cells. The single cells were then pre-plated for 2 h to remove fibroblasts at 5% CO2 and 37 °C. Then, the cardiomyocytes were cultured in Dulbecco's modified eagle medium (DMEM) medium (Gibco), supplemented with 10% fetal bovine serum (FBS, Gibco), and 1% penicillin–streptomycin (Gibco) at 5% CO2 and 37 °C for 48 h before further use.
8
Synthesis and Evaluation of PEG-OXA Prodrug
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OXA were supplied by Shandong Boyuan Pharmaceutical Co., Ltd. (Jinan, China). All other reagents used in the synthesis of the PEG-OXA prodrug were purchased from Sigma-Aldrich and used without further purification. Methanol (HPLC grade) was purchased from Fisher Scientific (Pittsburgh, USA). Formic acid (FA) was bought from Fluka Chemie (Buchs, Switzerland). Ultrapure water was prepared from a Milli-Q purification system (Millipore, MA, USA).
HT-29 and SW620 cells were purchased from China infrastructure of cell line resource (Beijing, China) and cultured with dulbecco’s modified eagle medium (DMEM) medium (Gibco) added 1% antibiotics and 10% fetal bovine serum (FBS, Gibco).
Sprague-Dawley rats (6–8 weeks old) and ICR mice (4–5 weeks old) were purchased from Pengyue Laboratory Animal Technology Co., Ltd (Jinan, China). Healthy BALb/c nude mice were bought from Gem Pharmatech Co., Ltd, and fed in a specific pathogen-free (SPF) animal laboratory. All the animal experiments reported in this paper were approved by the Committee on the Ethics of Animal Experiments of the Yantai Institute of Materia Medica, and was performed in strict accordance with the guidelines of the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
HT-29 and SW620 cells were purchased from China infrastructure of cell line resource (Beijing, China) and cultured with dulbecco’s modified eagle medium (DMEM) medium (Gibco) added 1% antibiotics and 10% fetal bovine serum (FBS, Gibco).
Sprague-Dawley rats (6–8 weeks old) and ICR mice (4–5 weeks old) were purchased from Pengyue Laboratory Animal Technology Co., Ltd (Jinan, China). Healthy BALb/c nude mice were bought from Gem Pharmatech Co., Ltd, and fed in a specific pathogen-free (SPF) animal laboratory. All the animal experiments reported in this paper were approved by the Committee on the Ethics of Animal Experiments of the Yantai Institute of Materia Medica, and was performed in strict accordance with the guidelines of the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
9
Bmal1 Deficiency in Myeloid Cells
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Bone marrow cells were obtained from the femurs and tibiae of 6–10 week old mice of both sexes. This included Bmal1LoxP/LoxP::Lyz2Cre (Bmal1myeloid−/−) which where compared with control Lyz2Cre (Bmal1myeloid+/+). This also included bone marrow harvest from PERIOD2::luciferase mice which were used for lumicycle analysis and also C57Bl/6J mice as WT BMDCs. Bone marrow cells were cultured in Dulbecco’s modified eagle medium (DMEM) medium (Gibco) supplemented with 10% foetal bovine serum (FBS) (Gibco), 100 U/ml penicillin, 100 μg/ml streptomycin, and 20 ng/ml GM-CSF (Biolegend, San Diego CA, USA) or 10% J558 cultured supernatants. Cells were maintained at 37 °C in a 5% CO2 atmosphere for 7 days, to allow for cell differentiation into BMDCs. Culture medium was freshly replaced every 2–3 days.
10
Bioactive Compounds in Isodon suzhouensis
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Isodon suzhouensis leaves were supplied by Suzhou
Lvyuan traditional Chinese Medicine Technology Co., Ltd. (Suzhou,
China). Acetonitrile, 95% ethanol, luminol, H2O2, and methanol were purchased from Aladdin. Penicillin–streptomycin
solution and fetal bovine serum were obtained from Biological Industries
(Beit-Haemek, Israel). The Dulbecco’s modified Eagle medium
(DMEM) medium was purchased from Gibco (Grand Island, NY, USA). The
BCA protein quantification kit, SOD assay kit, MDA assay kit, and
RIPI lysate were purchased from Beyotime (Shanghai, China). Antibodies
against p-p38, β-actin, and HRP-labeled goat anti-rabbit were
purchased from Bioworld. Antibodies for p38 and TNF-α were obtained
from Proteintech (Wuhan, China). Cigarettes are purchased locally,
and the oil content of each cigarette was 10 mg, the nicotine content
in the smoke was 0.9 mg, and the carbon monoxide in the smoke was
12 mg. For comparison, standard compounds of more than 98% purity
including glaucocalyxin A, glaucocalyxin B, isoquercetin, and rutin
were obtained from Shanghai Chen Gong Biotechnology Co., Ltd.
Lvyuan traditional Chinese Medicine Technology Co., Ltd. (Suzhou,
China). Acetonitrile, 95% ethanol, luminol, H2O2, and methanol were purchased from Aladdin. Penicillin–streptomycin
solution and fetal bovine serum were obtained from Biological Industries
(Beit-Haemek, Israel). The Dulbecco’s modified Eagle medium
(DMEM) medium was purchased from Gibco (Grand Island, NY, USA). The
BCA protein quantification kit, SOD assay kit, MDA assay kit, and
RIPI lysate were purchased from Beyotime (Shanghai, China). Antibodies
against p-p38, β-actin, and HRP-labeled goat anti-rabbit were
purchased from Bioworld. Antibodies for p38 and TNF-α were obtained
from Proteintech (Wuhan, China). Cigarettes are purchased locally,
and the oil content of each cigarette was 10 mg, the nicotine content
in the smoke was 0.9 mg, and the carbon monoxide in the smoke was
12 mg. For comparison, standard compounds of more than 98% purity
including glaucocalyxin A, glaucocalyxin B, isoquercetin, and rutin
were obtained from Shanghai Chen Gong Biotechnology Co., Ltd.
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