Anti blm

Manufactured by Fortis Life Sciences

The Anti-BLM is a laboratory equipment designed to detect the presence of the Black Lives Matter (BLM) protein or its related molecules in biological samples. The core function of this product is to provide researchers with a tool for the identification and quantification of the BLM target without making any interpretations or extrapolations about its intended use.

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Lab products found in correlation

3 protocols using anti blm

Protein Detection by Western Blotting

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Western blotting of whole cell extracts was performed according to standard procedures. Primary antibodies used were: anti-WRN (Abcam), anti-BLM (Bethyl Laboratories), anti-lamin B (Santa Cruz), anti-TRF2 (Imgenex), anti-p53 (Santa Cruz), anti-PARP1 p85 fragment (Promega), and anti-BRCA1 [60] (link). Secondary antibodies used were: horseradish-peroxidase conjugated goat-anti-mouse, goat-anti-rabbit and rabbit-anti-goat antibodies (Jackson ImmunoResearch Laboratories).

Gocha A.R., Acharya S, & Groden J. (2014). WRN Loss Induces Switching of Telomerase-Independent Mechanisms of Telomere Elongation. PLoS ONE, 9(4), e93991.

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Western Blot Analysis of DNA Damage Proteins

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Western blot analysis was performed as described [60 (link)]. Cells were lysed with NETN buffer (20 mM Tris, pH 8.0, 1 mM EDTA, 150 mM NaCl, and 0.5% Nonidet P-40) for 30 min. Cell lysates were boiled in 2xSDS loading buffer and subjected to SDS-PAGE.
Commercial antibodies used include anti-BLM (Bethyl Laboratories, A300), anti-H2AX-S139p (Cell Signaling, #2577), anti-Ras (Santa Cruz Biotechnology, sc-520), anti-FLAG (Sigma, F1804), anti-Ku70 (Santa Cruz Biotechnology, sc-17789), anti-β-actin (Sigma, A5441). Antibodies against FANCM was kindly provided by Dr. Weidong Wang [57 (link)].

Wang H., Li S., Zhang H., Wang Y., Hao S, & Wu X. (2018). BLM prevents instability of structure-forming DNA sequences at common fragile sites. PLoS Genetics, 14(11), e1007816.

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DNA Damage Response Pathway Profiling

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The following primary antibodies were used: anti-β-Actin (Thermo Fisher Scientific; #MA1-140), anti-pDNA-PKcs-S2056 (Thermo Fisher Scientific; #PA5-78130), anti-DNA-PKcs (Bethyl Laboratories; #A300-516A), anti-pRPA2-S4/8 (Bethyl Laboratories; #A300-245A), anti-RAD51 (Merck Millipore; #PC130), anti-pKAP1-S824 (Bethyl Laboratories; #A300-767A), anti-pATM-S1981 (Cell Signaling Technology; #13050S), anti-KAP1 (Bethyl Laboratories; #A300-274A), anti-pCHK1-S345 (Cell Signaling Technology; #2341), anti-CHK1 (Santa Cruz Biotechnology; #sc-8408), anti-pH2AX-S139 (Bethyl Laboratories; A300-081A), anti-BRCA1 (Cell Signaling Technology; #9010), anti-CTIP (Bethyl Laboratories; #A300-488A), anti-BLM (Bethyl Laboratories; #A300-110A), anti-V5 (Thermo Fisher Scientific; #R960-25). The secondary antibodies used were Goat anti-Mouse IgG-HRP (Thermo Fisher Scientific; #NA931V) and Donkey anti-Rabbit IgG-HRP (Thermo Fisher Scientific; #NA934V).

Dibitetto D., Sanchi A., Sanford E.J., Lopes M., & Smolka M.B. (2021). Fork Slowing and Reversal as an Adaptive Response to Chronic ATR Inhibition.

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