Slides with 10 μm thick pancreas sections were blocked in 10% Normal Donkey Serum (Jackson) diluted in 0.2% PBST (PBS, 0.2% Triton X-100) for one hour at room temperature. Following block, slides where incubated with primary antibodies diluted in 10% Normal Donkey Serum overnight at 4C. Slides were then washed 3 × 10 mins with 0.2% PBST and then incubated with secondary antibodies diluted in 10% Normal Donkey Serum for one hour at room temperature. Slides were then washed 3 × 10 minutes with 0.2% PBST. Primary antibodies used were Guinea pig anti-Insulin (1:6 Agilent), Rabbit anti-glucagon (1:200 CST), Rabbit anti-MafA (1:100, CST), Rabbit anti-Ucn3 (1:500, Phoenix), Rabbit anti-Glut2 (1:400, Sigma), and Rabbit anti-Ki67 (1:400; CST). Secondary antibodies used were Donkey anti Rabbit-488 (Jackson), and Donkey anti-Guinea pig 647 (Jackson).
Rabbit anti glut2
Manufactured by Merck Group
Rabbit anti-Glut2 is a laboratory reagent used for the detection and analysis of the Glut2 protein in biological samples. Glut2 is a glucose transporter found in various tissues, including the liver, kidney, and pancreatic beta cells. This product is a polyclonal antibody raised in rabbits against the Glut2 protein and can be used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to identify and quantify the presence of Glut2 in research samples.
Automatically generated - may contain errors
Lab products found in correlation
Normal donkey serum (nds), by Jackson ImmunoResearch (2 mentions)
Donkey anti rabbit 488, by Jackson ImmunoResearch (2 mentions)
Rabbit anti ucn3, by Phoenix Pharmaceuticals (2 mentions)
Donkey anti guinea pig 647, by Jackson ImmunoResearch (2 mentions)
Guinea pig anti insulin, by Agilent Technologies (2 mentions)
2 protocols using rabbit anti glut2
1
Immunohistochemical Profiling of Pancreatic Islets
2
Immunohistochemical Profiling of Pancreatic Islets
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Slides with 10 μm thick pancreas sections were blocked in 10% Normal Donkey Serum (Jackson) diluted in 0.2% PBST (PBS, 0.2% Triton X-100) for one hour at room temperature. Following block, slides where incubated with primary antibodies diluted in 10% Normal Donkey Serum overnight at 4C. Slides were then washed 3 × 10 mins with 0.2% PBST and then incubated with secondary antibodies diluted in 10% Normal Donkey Serum for one hour at room temperature. Slides were then washed 3 × 10 minutes with 0.2% PBST. Primary antibodies used were Guinea pig anti-Insulin (1:6 Agilent), Rabbit anti-glucagon (1:200 CST), Rabbit anti-MafA (1:100, CST), Rabbit anti-Ucn3 (1:500, Phoenix), Rabbit anti-Glut2 (1:400, Sigma), and Rabbit anti-Ki67 (1:400; CST). Secondary antibodies used were Donkey anti Rabbit-488 (Jackson), and Donkey anti-Guinea pig 647 (Jackson).
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