Accuzol total rna extraction kit

Manufactured by Bioneer

Sourced in Cameroon

The AccuZol Total RNA Extraction Kit is a laboratory equipment product designed to efficiently extract total RNA from a variety of biological samples. It utilizes a guanidinium-based lysis buffer to lyse cells and denature RNases, allowing for the isolation of high-quality, intact RNA suitable for downstream applications.

Automatically generated - may contain errors

Lab products found in correlation

Nanodrop, by Thermo Fisher Scientific (4 mentions) Cfx96 real time pcr detection system, by Bio-Rad (3 mentions) Ultrapure distilled water, by Thermo Fisher Scientific (2 mentions) Ampigene qpcr green mix, by Enzo Life Sciences (2 mentions) μdrop plate, by Thermo Fisher Scientific (2 mentions) Cdna synthesis kit, by Bioneer (2 mentions) Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Lightcycler 96 instrument, by Roche (1 mentions) Ssofast evagreen supermix, by Bio-Rad (1 mentions) Corbett 65h0 machine, by Qiagen (1 mentions)

Close spelling variants of this product

AccuZolTM RNA extraction kit AccuZolTM kit RNA extraction kit Accuzol

6 protocols using accuzol total rna extraction kit

Relative Quantification of mRNA Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the relative estimation of mRNA expression, total RNA was extracted from fresh frozen
in liquid nitrogen tissue samples using the AccuZol Total RNA Extraction Kit (Bioneer,
Korea), and then cDNA was synthesized based on the manufacturer’s recommendations using a
commercially available kit (Fermentas, Thermo Fisher Scientific, Waltham, USA). The
real-time PCR was carried out using RealQ Plus 2×Master Mix Green (Amplicon, Denmark) on
the LightCycler® 96 Instrument (Roche, Life Science, Sandhofer, Germany). Briefly, PCR
reactions were conducted at 20 µl as final volume, containing SYBR Green master mix (10
µl), 1 µl cDNA, and 2 µl of specific primers, and 7 µl ddH₂O. The PCR
reactions were set at the following program: 95°C (5 minutes), followed by 40 cycles at
95°C (30 seconds), 57°C (30 seconds), and 72°C (40 seconds). To analyze the relative gene
expression data for mRNA, we used the Livak-Schmittgen (22 (link)) equation to calculate the
2^−ΔΔCT, which compares two values in the exponent, then the obtained data
were used for further analysis of gene expression levels. Specific primers were designed
using the Primer-BLAST tool available from the NCBI for target genes sequences in the
GenBank databases (www.ncbi.nlm.nih.gov). The primer sets (forward and reverse) for
real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) are
presented in supplementary Table 1.

Ghassemi H., Hashemnia M., Mousavibahar S.H., Mahmoodzadeh Hosseini H, & Mirhosseini S.A. (2021). Positive Association of Matrix Proteins Alteration with TAZ and The Progression of High-Grade Bladder Cancer. Cell Journal (Yakhteh), 23(7), 742-749.

+ Open protocol

+ Expand

Quantitative Real-Time PCR Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gene expression was analyzed to confirm the differentially expressed protein expression. RNA was isolated using Accuzol Total RNA extraction kit (Bioneer, Daejeon, Korea), and the concentration and purity were determined using a µDrop plate (NanoDrop; Thermo Fisher Scientific). From 1 µg of the total RNA, complementary DNA was synthesized using a cDNA synthesis kit (AccuPower Cycle RT premix, dt20; Bioneer, Daejeon, Korea). The primers were designed using Primer 3 software (Primer3 v.0.4.0) (Table S2). RT-qPCR was performed using SsoFast EvaGreen Supermix (Biorad, Hercules, CA, USA) in a CFX96 real-time PCR detection system (Biorad). The RT-qPCR thermal cycle conditions were as follows: 95 °C for 5 min, followed by 40 cycles at 95 °C for 5 s for denaturation, and 40 cycles at 60 °C for 30 s for annealing and extension. The results were expressed as Ct values normalized against GAPDH, and their gene expression levels were calculated using the 2^−ΔΔCt method [15 (link)].

Kang D, & Shim K. (2021). Early Heat Exposure Effects on Proteomic Changes of the Broiler Liver under Acute Heat Stress. Animals : an Open Access Journal from MDPI, 11(5), 1338.

+ Open protocol

+ Expand

Evaluating CTGF mRNA Expression in Rabbit Trabeculectomy

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the expression of CTGF mRNA in the bleb area and compare its expression at different time intervals after trabeculectomy, total RNA was extracted from five different rabbit eye groups which included one intact eye without any surgery, one eye 5 hr after trabeculectomy, one eye five days after trabeculectomy with no injection, one eye five days after trabeculectomy with the administration of a BSS injection, and finally one eye five days after trabeculectomy with MMC injection. The globe sections including the trabeculectomy areas in the operated eyes were homogenized and the AccuZol total RNA extraction kit (K-3090, Bioneer, Korea) was used to extract the total RNA. The concentration/purity and the integrity of the isolated RNA were determined using a NanoDrop instrument (Thermo Scientific, Waltham, MA, USA) and agarose gel electrophoresis, respectively. cDNA was then generated by reverse transcription of the total RNA using a Revert Aid First Strand cDNA Synthesis Kit (#K1621, Thermo Scientific, Waltham, MA, USA). Subsequently, real-time PCR was performed utilizing a Corbett 65H0 machine (Corbett Research, Sidney, Australia) using the SinaSYBR Blue HS-qPCR Mix (#MM2171, Sinaclon, Tehran, Iran). B2M (beta-2 microglobulin) gene expression was quantified as the reference gene. Real-time PCR primers sequences used in this study are listed in Table 1.

Hassanpour K., Kanavi M.R., Daftarian N., Samaeili A., Suri F., Pakravan M., Doozandeh A., Aski S.A., Fakhri M., Moghaddasi A., Ahmadieh H, & Esfandiari H. (2022). The Inhibitory Effect of Connective Tissue Growth Factor Antibody on Postoperative Fibrosis in a Rabbit Model of Trabeculectomy. Journal of Ophthalmic & Vision Research, 17(4), 486-496.

+ Open protocol

+ Expand

RNA Extraction and qRT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

JBP muscle cells were collected at passages of P6 to P10. Total RNAs were extracted from these cells using an AccuZol Total RNA extraction kit (Bioneer, Daejeon, Korea). Quantity and purity of RNAs were determined using a spectrophotometer (μDrop plate, Thermo Fisher Scientific, USA). One microgram of total RNA was reverse-transcribed to cDNA with a cDNA synthesis kit (Bioneer, Korea). Quantitative real-time polymerase chain reaction (qRT-PCR) assays were performed using 1 μL of cDNA and 19 μL of stock solution containing AMPIGENE qPCR Green Mix (Enzo, San Diego, CA, USA), UltraPure distilled water (Invitrogen, USA), and primer solution containing both sense and antisense custom-designed primers on a CFX96 real-time PCR detection system (Bio-Rad, USA). Samples were denatured at 95°C for 5 minutes and cycled 40 times at 95°C (for denaturing) for 5 seconds and 60°C (for annealing and extension) for 30 seconds. Gene-specific primer sequences are listed in Table 1. The qRT-PCR results were normalized against GAPDH as a housekeeping gene to calculate the expression of each target gene.

Park J., Lee J., Song K.D., Kim S.J., Kim D.C., Lee S.C., Son Y.J., Choi H.W, & Shim K. (2021). Growth factors improve the proliferation of Jeju black pig muscle cells by regulating myogenic differentiation 1 and growth-related genes. Animal Bioscience, 34(8), 1392-1402.

+ Open protocol

+ Expand

RNA Extraction and qRT-PCR Analysis of miRNA Pathway Genes

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The RNA isolation was performed in peripheral blood samples of all study groups using the AccuZol™ Total RNA extraction kit (Bioneer Corp., Daejeon, South Korea), and the RNAs were translated to complementary deoxyribonucleic acid (cDNA) with high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific, MA, USA).
Each cDNA sample was analyzed for gene expressions in ExiCycler™ 96 Quantitative-PCR device (Bioneer Corp., Daejeon, South Korea) by RT-PCR using the K-6253 AccuPower® 2X GreenStar™ Master Mix kit (Bioneer Corp., Daejeon, South Korea) and specific primers designed with reference to the literature on DICER1 (Dicer), Drosha, DGCR8, XPO5, and AGO2 (Table 1).[16 (link)-18 (link)]

Çabukusta Acar A., Yoldaş Ş.B., Gencer E.S., Aycan İ.Ö, & Sanlı S.H. (2023). The relationship between prognosis of patients with traumatic brain injury and microRNA biogenesis proteins. Turkish Journal of Trauma & Emergency Surgery, 29(11), 1228-1236.

+ Open protocol

+ Expand

Quantitative Real-Time PCR Analysis of JBP Muscle Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

JBP muscle cells were collected at passages of P6 to P10. Total RNAs were extracted from these cells using an AccuZol Total RNA extraction kit (Bioneer, Daejeon, Korea). Quantity and purity of RNAs were determined using a spectrophotometer (μDrop plate, Thermo Fisher Scientific, San Jose, CA, USA). One microgram of total RNA was reverse-transcribed to cDNA with a cDNA synthesis kit (Bioneer, Daejeon, Korea). Quantitative realtime polymerase chain reaction assays were performed using 1 uL of cDNA and 19 uL of stock solution containing AMPIGENE ® qPCR Green Mix (Enzo, San Diego, CA, USA), UltraPure distilled water (Invitrogen, Carlsbad, CA, USA), and primer solution containing both sense and antisense custom-designed primers on a CFX96™ real-time PCR detection system (Bio-Rad, Hercules, CA, USA). Samples were denatured at 95 for 5 minutes and cycled 40 times at 95 (for denaturing) for 5 seconds and 60 (for annealing and extension) for 30 seconds. Gene-specific primer sequences are listed in Table 1. qRT-PCR results were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a housekeeping gene to calculate the expression of each target gene.

Park J., Lee J., Song K., Kim S., Choi H.W., & Shim K.S. (2020). Growth factors improve the proliferation of Jeju black pig muscle cells by regulating MyoD and growth-related genes.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!