The MCF-7 cells were plated in 6-well plates at 37 °C in a humidified atmosphere containing 5% CO2. After 24 h, the cells were treated with 25 and 50 μM SSF2-2 and incubated for 24 h at 37 °C in a humidified atmosphere containing 5% CO2. For determining apoptotic cell death, the cells were stained with 5 μL annexin V and 1 μL propidium iodide (PI) for 30 min at RT using a Tali Image-Based Cytometer Apoptosis Kit (Invitrogen, Life Technologies, Carlsbad, CA, USA). The percentage of apoptotic cells was counted in 10 randomly selected fields per slide at a 40× magnification using a Tali Image-Based Cytometer (Invitrogen; Temecula, CA, USA).
Tali image based cytometer apoptosis kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Tali Image-based Cytometer Apoptosis Kit is a lab equipment product that enables the detection and measurement of apoptosis in cells. It provides a quantitative analysis of apoptotic cells using fluorescence-based imaging techniques.
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Lab products found in correlation
2 protocols using tali image based cytometer apoptosis kit
1
Assessing Apoptosis in MCF-7 Cells
2
Apoptosis Analysis of Prostate Cancer Cells
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To evaluate the degree of apoptosis, the Tali image-based cytometer apoptosis kit—annexin V Alexa Fluor 488 and propidium iodide—was used according to the manufacturer's instructions (Invitrogen/Life Technologies, Carlsbad, CA, USA). In brief, DU145 and PC3 were incubated at a density of 2.5 × 105 in a 6-well plate for 16 hour and then treated with paclitaxel (5 nM), naringin (150 μΜ), and their combination. The cells were collected 72 hour posttreatment and washed twice with ice-cold phosphate buffer saline (PBS) and were evaluated by Tali image-based cytometer within 30 minutes.
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