The following antibodies were purchased from Abcam (Cambridge, United Kingdom): ADAM10 (ab1997, 1:1000), DDX17 (ab180190, 1:1000), and BACE1 (ab2077, 1:1000). Antibodies against APP and CTFs (A8717, 1:1000) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and those against GADPH (60004-1-Ig, 1:10,000) were obtained from Proteintech (Wuhan, China).
Ddx17 ab180190
Manufactured by Abcam
Sourced in United Kingdom
DDX17 (ab180190) is a recombinant protein that can be used as a research tool. DDX17 is a member of the DEAD-box protein family, which are RNA helicases involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
A8717, by Merck Group (1 mentions)
Adam10, by Abcam (1 mentions)
Ab2077, by Abcam (1 mentions)
Ripa buffer, by Keygen Biotech (1 mentions)
Tgif2, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Chemidoc xr, by Bio-Rad (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
2 protocols using ddx17 ab180190
1
Antibody Sources for APP and CTF Detection
2
Western Blot Analysis of PCa Cell Lysates
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Western blot analysis was conducted as described previously [18 (link)]. Briefly, PCa cells were lysed in RIPA buffer (#KGP250, KeyGEN) following the manufacturer’s instructions. Then, 25 µg of protein was separated by SDS‒PAGE and transferred to PVDF membranes (Millipore). Subsequently, membranes were blocked with 5% nonfat milk. After washing, the primary antibodies were immunoblotted. The following primary antibodies were used in this research: DDX17 (ab180190) was purchased from Abcam, and TGIF2 (sc -81,989) and GAPDH (sc -365,062) were purchased from Santa Cruz. After incubation overnight, HRP-conjugated anti-mouse (sc-2005) and anti-rabbit IgG (sc-2004) secondary antibodies (Santa Cruz) were added. Finally, the signals were visualized by Bio-Rad ChemiDoc XRS+.
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