Il 13rα1

Manufactured by Abcam

Sourced in United Kingdom

IL-13Rα1 is a cell surface receptor that binds to the cytokine interleukin-13 (IL-13). It is a component of the IL-13 receptor complex and is involved in the signaling pathways activated by IL-13.

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Lab products found in correlation

P akt ser473, by Cell Signaling Technology (1 mentions) Bradford protein assay kit, by Bio-Rad (1 mentions) P mtor ser2448, by Cell Signaling Technology (1 mentions) Filaggrin, by Abcam (1 mentions) Involucrin, by Abcam (1 mentions) β actin, by Abcam (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Loricrin, by Santa Cruz Biotechnology (1 mentions) P s6k1 thr389, by Cell Signaling Technology (1 mentions) Hexokinase 2, by Abcam (1 mentions) Dab substrate, by Thermo Fisher Scientific (1 mentions) Il 13, by Abcam (1 mentions) Interleukin 4 (il 4), by Abcam (1 mentions) C myc, by Abcam (1 mentions) Hrp polymers, by Biocare Medical (1 mentions)

2 protocols using il 13rα1

Protein Expression Analysis in Keratinocytes

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Total protein was extracted from HaCaT cells and protein concentration was determined using Bradford protein assay kit (Bio-Rad Laboratories). 20 μg of protein extracts were separated by 10% SDS-PAGE and electrotransferred onto a polyvinyllidenedifluoride membrane. The membranes were incubated overnight at 4 °C with primary antibodies against IL-13Rα1 (1:1000; Abcam, Cambridge, UK), p-S6K1 (Thr389, 1:1000, Cell Signaling Technology, USA), S6K1 (1:1000, Cell Signaling Technology, USA), p-Akt (Ser473, 1:2000, Cell Signaling Technology, USA), Akt (1:1000; Abcam, Cambridge, UK), p-mTOR (Ser2448, 1:1000, Cell Signaling Technology, USA), mTOR (1:1000, Cell Signaling Technology, USA), filaggrin (1:1000; Abcam, Cambridge, UK), loricrin (1:200; Santa Cruz Biotechnologies, CA, USA), involucrin (1:1000; Abcam, Cambridge, MA, USA), and β-actin (1:1000; Abcam, Cambridge, UK). The secondary antibodies (Sigma-Aldrich, St. Louis, MO) were incubated in 5% skimmed milk powder. The SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific Inc) was used to visualize the signals.

Jia Q.N, & Zeng Y.P. (2020). Rapamycin blocks the IL-13-induced deficiency of Epidermal Barrier Related Proteins via upregulation of miR-143 in HaCaT Keratinocytes. International Journal of Medical Sciences, 17(14), 2087-2094.

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Immunohistochemical Profiling of Tumor Samples

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Harvested tissues were immediately fixed in 10% formalin overnight and embedded in paraffin. IHC was performed as described previously^{62 (link)}. Briefly, endogenous peroxidases were inactivated by 3% hydrogen peroxide. Non-specific signals were blocked using 3% BSA, 10% goat serum in 0.1% Triton X-100. Tumor samples were stained with the following primary antibodies: IL2Rγ (Abcam ab180698, Bioss bs-2545R); Ki67 (Vector Laboratory, VP-RM04); IL4R (Bioss bs2458R); cMyc (Abcam ab32072); Hexokinase II (Abcam ab209847); Gata-3 (CST 5852); LDHA (CST 3582); IL13rα1 (Abcam ab79277); Jak1 (CST 3344); pSTAT1-Y701 (CST 9167); pSTAT3-Y705 (CST 9145); Stat5 (CST 94205); Stat1 (CST 9172); IL4 (Abcam ab9622); IL13 (Abcam ab106732); CD45 (Abcam ab10558), CD4 (Abcam ab183685) and F4/80 (Abcam ab6640). After overnight incubation, the slides were washed and incubated with secondary antibody (HRP-polymers, Biocare Medical) for 30 min at room temperature. The slides were washed three times and stained with DAB substrate (ThermoFisher Scientific). The slides were then counterstained with haematoxylin and mounted with mounting medium. For clinical samples, staining intensity of tissue sections was scored in a ‘blinded’ manner by two independent pathologists.
Immunofluorescence slides were imaged with an Olympus Microscope and quantified with ImageJ.

Dey P., Li J., Zhang J., Chaurasiya S., Strom A., Wang H., Liao W.T., Cavallaro F., Denz P., Bernard V., Yen E.Y., Genovese G., Gulhati P., Liu J., Chakravarti D., Deng P., Zhang T., Carbone F., Chang Q., Ying H., Shang X., Spring D.J., Ghosh B., Putluri N., Maitra A., Wang Y.A, & DePinho R.A. (2020). Oncogenic Kras driven metabolic reprogramming in pancreas cancer cells utilizes cytokines from the tumor microenvironment. Cancer discovery, 10(4), 608-625.

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