GR, TH, and SYN mRNA expression was quantified in the SN and the ventral tegmental area, along with GR and calcyon mRNA expression in striatum and the motor cortex, by measuring optical density on autoradiograms. Images were captured with a flatbed scanner (Perfection 1260, Epson, Long Beach, CA), saved in TIF format, and processed with Adobe Photoshop 6.0 (Adobe Systems Inc., Mountain View, CA). Processing of images included color inversion. Optical density was measured using the public domain NIH Image program (Version 1.63, National Institutes of Health, Bethesda, MA).
Perfection 1260
Manufactured by Epson
Sourced in Japan
The Epson Perfection 1260 is a flatbed scanner designed for scanning documents, photographs, and other media. It has a maximum optical resolution of 1200 dpi and can scan in 48-bit color depth. The scanner uses a CCD (Charge-Coupled Device) sensor technology and is capable of scanning at a range of resolutions to accommodate various scanning needs.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using perfection 1260
1
Quantifying mRNA Expression in Brain Regions
2
Quantification of Brain Infarct Volume
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Brain infarct volume was quantified by 2,3,5-triphenyltetrazolium chloride (TTC) staining [30 (link), 31 (link)]. Rats were euthanized with an overdose of pentobarbital at 7 days post-operation. The brains of the rats were harvested and cut into slices of 2 mm in thickness. The fresh brain slices were immersed in 2 % TTC solution in PBS for 20 min at 37 °C and their images were subsequently captured using an Epson Perfection 1260 desktop scanner (Seiko Epson Corporation, Nagano, Japan) at a resolution of 600 dpi. The area of the white infarct region was then analyzed using Adobe Photoshop CS4 Extended Version 11.0.1 (Adobe Systems Inc., San Jose, CA, USA). The infarct volume of a slice was calculated using the following equation: The total infarct volume was calculated by summing up the infarct volumes of the different slices.
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