E200 microscope

Young-adult worms of wild-type (N2), nhr-49(nr2041), and ador-1(ox489) were exposed to 100 uM of juglone, also called 5-hydroxy-1,4- naphthoquinone (IUPAC), a generator of reactive oxygen species (ROS) [37 (link)], this concentration is supposed to kill approximately 50% of the nematodes (LD50), after 1 hour exposure [38 ]. Juglone was prepared in EtOH (1% final concentration). After 1 hour at 20°C, 100 nematodes per treatment with Ilex paraguariensis were assessed with a Nikon E200 microscope (Tokyo, Japan). Animals that reacted to a mechanical stimulus were scored as alive, and non-responding animals were considered to be dead (Fig 5). Analyses were carried out in five independent assays. Results are shown as percentage of alive animals.

Freshly excised hearts and skeletal muscles were cut in half along the transverse plane and placed into OCT medium to be frozen in liquid nitrogen-cooled isopentane (− 160 °C) and stored at − 80 °C. Short-axis cross-sections of the hearts were sectioned at 7 µm and stained with hematoxylin and eosin using standard histochemical procedures. Images were collected on a Nikon E200 microscope.

The sections of BCa and normal adjacent tissues were deparaffinized and permeabilized. Then, the specimens were incubated with the anti-TNS1 antibody (Proteintech, Wuhan, China) overnight at 4^oC. Then, we incubated the specimens with the secondary antibody (Proteintech, Wuhan, China). The color was developed with DBA and the stained samples were imaged with a Nikon E200 microscope.

Wings were dissected dry, transferred to glass microscope slide with isopropanol and mounted in AquaPolymount (Polysciences, Inc.). Anterior wing margin bristles and sensillae were viewed under brightfield optics using a Nikon E200 microscope at 400× magnification. For each wing, we counted the number of twin campaniform sensillae, stout bristles, dorsal recurved bristles, ventral recurved bristles, ventral slender bristles, and domed sensillae on longitudinal vein L3 and on the anterior crossvein. Sample size for each genotype varied from 5 to 32. Median values were subjected to a Kruskal-Wallis test. For those traits with a P-value < 0.001, a Mann-Whitney U test was performed on paired combinations of all genotypes.

Bacterial suspension (10 µL) was collected after the incubation period in a PHA production medium, placed on a microscope slide, air dried, and fixed by heating in a Bunsen burner flame. The so-fixed smear was stained with a SYBR-Nile Red solution in 50% methanol for 15 min. Afterward, the glass slide was rinsed with ddH₂O and air-dried in darkness. A cover slide was fixed on top of the smear with a drop of CitiFluor AF1 anti-fade solution. Cells were observed under 1000 magnification with green (510–560 nm) and blue light (450–490 nm) excitation on a Nikon E-200 microscope with a 100-W Hg lamp and 100 × CFI 60 oil immersion objective. Images were captured with a digital DS-Fi3 high-definition color microscope camera equipped with a 5.9-megapixel CMOS image sensor and a filter block of wavelengths: EX 330–380, DM 400, BA 420.