Rabbit chondrocytes were obtained from the New Zealand white rabbits. Cetyltrimethylammonium bromide (CTAB) was obtained from Alfa Aesar. Gold chloride trihydrate (HAuCl4∙3H2O), sodium borohydride (NaBH4), Silver nitrate (AgNO3), ascorbic acid, chitosan, calcein AM, MitoTracker and sodium alginate were purchased from Sigma-Aldrich. HSP 27, HSP 27 and HSP 90 were obtained from abcam.
Mitotracker
Manufactured by Merck Group
Sourced in United States
MitoTracker is a fluorescent dye that selectively accumulates in active mitochondria. It is used to label and visualize mitochondria in live cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Hsp90, by Abcam (1 mentions)
Cetyltrimethylammonium bromide ctab, by Thermo Fisher Scientific (1 mentions)
Hsp27, by Abcam (1 mentions)
Calcein am, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Sodium alginate, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Silver nitrate, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Gold chloride trihydrate, by Merck Group (1 mentions)
Bodipy 493 503, by Merck Group (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Mitotracker, by Thermo Fisher Scientific (1 mentions)
Ultraview vox, by PerkinElmer (1 mentions)
Flowing software, by De Novo Software (1 mentions)
Fluorescence activated cell sorting (facs), by Sysmex (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt hoechst 33342, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
7 protocols using mitotracker
1
Rabbit Chondrocytes and Nanoparticle Synthesis
2
Visualizing Lipids and Mitochondria in Neonatal Mouse Cardiomyocytes
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Neonatal mouse cardiomyocytes were washed with 1 ml of PBS to remove the medium. The cells were incubated with 200 nM MitoTracker (Sigma) stain for 20 min in the dark at room temperature. The cells were washed with 1 ml of PBS three times. The cells were fixed with 4% paraformaldehyde for 20 min. Then, the cells were incubated with 2 μM BODIPY 493/503 (Sigma) staining solution in the dark for 20 min at 37 °C. The cells were washed using 1 ml of PBS to remove the staining solution. Finally, the nuclei were stained with Hoechst 33258 (Sigma) for 5 min. The cells were observed and photographed by fluorescence microscopy (Olympus IX83). Quantification analysis of lipid contents and mitochondria was performed using Image-Pro Plus 6.0 (Media Cybernetic Inc., USA) image analysis software.
3
Mitochondrial Dynamics in Oocyte Maturation
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Activated mitochondria were imaged with 0.1 µM MitoTracker (Invitrogen, M7512, China), a cell‐permeable potential‐sensitive fluorescent mitochondrial dye emitting in the red (561 nm excitation, 590 nm emission) channel, which had a high affinity with higher potential mitochondria, by using a time‐lapse confocal laser microscope (UltraVIEW‐VoX; PerkinElmer, USA). The GV oocyte meiotic maturation was conducted by incubation in an M2 medium (M7167, Sigma‐Aldrich, USA) containing 0.1 µM MitoTracker.
4
Mitochondrial Imaging in Cultured MSCs
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MitoTracker (Sigma-Aldrich, St. Louis, MO, USA) was used to observe mitochondria in cultured MSCs. The cells were incubated with MitoTracker (10 nM) for 15 min. After washing with PBS twice, these cells were suspended in 500 μL PBS, and were used to detect the mitochondrion ratio per cell by using FACS (Sysmex, Kobe, Japan). Cell forward scatter levels indicating MitoSOX™ positive cells were analyzed using Flowing Software (De Novo Software, Los Angles, CA, USA).
5
Anticancer Potential of Decursinol Angelate
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Decursinol angelate (Item no: 25212), (PubChem CID: 776123) was purchased from Cayman (Cayman Chemicals, Ann Arbor, Michigan, United States). DMEM, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Hoechst 33342, dichlorodihydrofluorescein diacetate (H2DCFDA), acridine orange (AO), dimethylsulfoxide (DMSO) and mitotracker were purchased from Sigma (Sigma-Aldrich, St. Louis, MI, USA). Fetal bovine serum was purchased from Gibco, (Gibco-Thermo Fisher, Waltham, MA, USA). Details of the primary and secondary antibodies used are mentioned in Supplementary Table S1 . All the other solvents used for the study were of highest grade and supplied by Sigma (Sigma-Aldrich).
6
Decursinol Angelate Protocol for Cell Studies
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Decursinol angelate (DA) (PubChem CID: 776123) was purchased from Cayman Chemicals (Item no: 25212); RPMI-1640, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), ethidium bromide and mitotracker were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA); and glutamate dehydrogenase (EC number: EC 1.4.1.2.). Fetal bovine serum was purchased from Gibco, USA. The complete details of all the primary and secondary antibodies used in this experiment can be found in a Tables S1 and S2 in the Supplementary Materials . All the other solvents used for the study were of highest grade and supplied by Sigma (Sigma-Aldrich, St. Louis, MO, USA).
7
Mitochondrial Dynamics in PINK1-KO Cells
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TUFm +/À and pink1-KO cell lines were treated with CCCP or DMSO for 0, 2, 4, 6, 8, 10 h and then conducted western-blot analysis with anti-NDUFS3 (Abcam, ab14711, clone 17D95) and anti-Tubulin (DSHB, AB2315513). Moreover, mitochondrial contents in control or pink1-KO HeLa cells were also visualized with Mito Tracker Red (Molecular Probes) using a Leica SP5 confocal microscopy. Cells were co-transfected with GFP and indicated plasmids for 48 h and then treated with CCCP (Sigma-Aldrich, C2759) or DMSO for 8 h following stained with Mito Tracker for 30 min. Finally, the mitochondrial content were assessed with Mito-Morphology Macro in ImageJ as previously described (Dagda et al., 2009) . More than 30 randomly selected individual cells were analyzed for each data point. Three biological replicates were performed.
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