Anti opa1

Manufactured by Thermo Fisher Scientific

Anti‐Opa1 is a laboratory reagent used for the detection and quantification of the Opa1 protein in biological samples. Opa1 is a mitochondrial protein involved in the regulation of mitochondrial dynamics. The Anti‐Opa1 product provides a tool for researchers to study Opa1 expression and function in their experiments.

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Lab products found in correlation

Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (2 mentions) Anti mfn2, by Santa Cruz Biotechnology (2 mentions) Anti mfn1, by Santa Cruz Biotechnology (2 mentions) Ripa buffer, by Beyotime (1 mentions) Anti src, by Abcam (1 mentions) Protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Anti p src, by Santa Cruz Biotechnology (1 mentions) Anti stat3, by Abcam (1 mentions) Ibright 1500 imaging system, by Thermo Fisher Scientific (1 mentions) Anti bcl 2, by Santa Cruz Biotechnology (1 mentions) Anti citrate synthase, by Santa Cruz Biotechnology (1 mentions) Pierce ecl kit, by Thermo Fisher Scientific (1 mentions) Anti drp1, by Cell Signaling Technology (1 mentions) Anti vdac, by Santa Cruz Biotechnology (1 mentions) Anti gpx1, by Thermo Fisher Scientific (1 mentions) Anti mul1, by Abcam (1 mentions) Anti p drp1, by Cell Signaling Technology (1 mentions) Anti sod2, by GeneTex (1 mentions) Anti catalase, by GeneTex (1 mentions) Anti fis1, by Santa Cruz Biotechnology (1 mentions)

4 protocols using anti opa1

Western Blot Analysis of Protein Signaling

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Proteins were extracted using RIPA buffer (Beyotime Biotechnology) containing protease and phosphatase inhibitor cocktail (Cat# 78446, Thermo) and boiled in 1 × loading buffer, then separated by SDS–polyacrylamide gels and transferred to PVDF membranes. Anti‐CD96 (3 µg mL, Cat# PA5‐97568, Thermo), anti‐Stat3 (1:1000, Cat# ab119352, Abcam), anti‐p‐Stat3 (1:1000, Cat# 4074, CST), anti‐Src (1:1000, Cat# ab109381, Abcam), anti‐p‐Src (1:1000, Cat# sc166860, Santa Cruz Biotechnology), anti‐p85 (1:1000, Cat# 4292, CST), anti‐p‐P85 (1:800, Cat# PA5‐104853, Thermo), anti‐JAK (1:1000, Cat# 3344, CST), anti‐p‐JAK (1:1000, Cat# 3771, CST), anti‐Opa1 (1:1500, Cat# PA5‐98029, Thermo), anti‐CD155 (1:1000,Cat # PA5‐96414, Thermo), or anti‐GAPDH (1:5000, Cat# 8884,CST) was diluted in TBST containing 5% BSA and incubated with membranes overnight at 4 °C. Peroxidase‐conjugated secondary antibody (1:3000, Cat# 7074 or Cat# 7076, CST) was co‐cultured with membranes and the antigen–antibody reactions were visualized with enhanced chemiluminescence assays (ECL, Thermo).

Li J., Xia Q., Di C., Li C., Si H., Zhou B., Yu S., Li Y., Huang J., Lu Y., Huang M., Liang H., Liu X, & Zhao Q. (2022). Tumor Cell‐Intrinsic CD96 Mediates Chemoresistance and Cancer Stemness by Regulating Mitochondrial Fatty Acid β‐Oxidation. Advanced Science, 10(7), 2202956.

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Quantitative Western Blot Analysis

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The western blotting procedures were fully described previously.
¹⁴ (link) The following primary antibodies were used: anti‐4‐HNE (Abcam, AB46545, 1:4000), anti‐Bax (Santa Cruz, Sc‐7480, 1:100), anti‐Bcl‐2 (Santa Cruz, Sc‐7382, 1:200), anti‐catalase (GeneTex, GTX110704, 1:1000), anti‐citrate synthase (Santa Cruz, Sc‐390693, 1:200), anti‐p‐DRP1 (Ser616, Cell Signaling, #34555, 1:500), anti‐DRP1 (Cell Signaling, #5391S, 1:500), anti‐Fis1 (Santa Cruz, Sc‐376447, 1:200), anti GPx1 (Thermo Fisher Scientific, PA5‐26323, 1:1000), anti‐MFN2 (Santa Cruz, Sc‐100560, 1:200), anti‐Mul1 (Abcam, AB209263, 1:1000), anti‐OPA1 (Thermo Fisher Scientific, MA5‐16149, 1:1000), anti‐Parkin (Abcam, AB77924, 1:2000), anti‐PINK1 (Thermo Fisher Scientific, PA1‐16604, 1:500), anti‐PGC‐1α1 (Millipore, AB3242, 1:1000), anti‐SOD2 (Genetex, GTX116093, 1:1000) and anti‐VDAC (Santa Cruz, Sc‐390996, 1:200). Then, anti‐rabbit (Cell Signaling, #7074S, 1:4000) or anti‐mouse (Cell Signaling, #7076S, 1:4000) secondary antibodies were used. The blots were revealed using a Pierce ECL kit (Thermo Fisher Scientific) or SupraSignal Femto kit (Thermo Fisher Scientific), and proteins were visualized by enhanced chemiluminescence (iBright 1500 Imaging System, Invitrogen) and quantified with ImageJ Software (version 1.8.0). Ponceau coloration was used as the loading control.

Mallard J., Hucteau E., Bender L., Moinard‐Butot F., Rochelle E., Boutonnet L., Grandperrin A., Schott R., Pflumio C., Trensz P., Kalish‐Weindling M., Charles A., Gény B., Favret F., Pivot X., Hureau T.J, & Pagano A.F. (2024). A single chemotherapy administration induces muscle atrophy, mitochondrial alterations and apoptosis in breast cancer patients. Journal of Cachexia, Sarcopenia and Muscle, 15(1), 292-305.

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Western Blot Analysis of Mitochondrial Dynamics

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Cells were lysed in RIPA lysis buffer (AMRESCO) plus protease inhibitor cocktail (Roche). 30 μg of total protein extracts were resolved on a SDS-electrophoresis gel and transferred to nitrocellulose membranes. After blocking process, the membranes were incubated with rabbit polyclonal anti-OPA1 (Thermo Fisher 1:1,000 dilution), rabbit polyclonal anti-DRP1 (Thermo Fisher 1:1,000) and rabbit polyclonal anti-MFN1 (Santa Cruz 1:1,000) antibodies. The equal loading and transfer of membranes were subsequently re-tested with anti-β-actin (Thermo Fisher 1:2,000). After treatment with HRP-linked goat anti-mouse or anti-rabbit secondary antibodies (Thermo Fisher 1:2,000) as indicated, immunoreactive proteins were detected using enhanced chemiluminescence reactive (ECL, Thermo Fisher).

Pérez M.J., Ponce D.P., Osorio-Fuentealba C., Behrens M.I, & Quintanilla R.A. (2017). Mitochondrial Bioenergetics Is Altered in Fibroblasts from Patients with Sporadic Alzheimer's Disease. Frontiers in Neuroscience, 11, 553.

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Mitochondrial Protein Expression Analysis

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The primary antibodies used were as follows: Anti-β-tubulin (sc-9104, Santa Cruz Biotechnology, Inc., 1:2,000), anti-Total OXPHOS Human WB Antibody Cocktail (ab110411, Abcam, Inc. 1:2,000), anti-COX IV (11967S, Cell Signaling, 1:1,000), anti-β-actin (sc-47778, Santa Cruz Biotechnology, Inc., 1:1,000), anti-Opa1 (PA1-16991, Thermo Fisher Scientific, 1:1,000), anti-Cyclophilin D (sc-376061, Santa Cruz Biotechnology, Inc., 1:1,000), anti-ANT (Santa Cruz biotechnology; 1:1,000), anti-Mfn1 (sc-50330, Santa Cruz Biotechnology, Inc. 1:1,000), anti-Mfn2 (sc-50331, Santa Cruz Biotechnology, Inc., 1:1,000), anti-phospho-DRP1 (Ser616) (4494, Cell Signaling, 1:1,000), anti-DRP1 (sc-271583, Santa Cruz Biotechnology, Inc., 1:1,000), anti-nitrotyrosine (141682, US Biological, Life Sciences, 1:500), and anti-4HNE (H6275-02, US Biological, Life Sciences, 1:1,000). The fluorescent dyes used were as follows: MitoTracker^TM Red CM-H2Xros (M7513, Thermo Fisher Scientific), MitoTracker^TM Green FM (M7514, Thermo Fisher Scientific), and VECTASHIELD Mounting Medium with DAPI (H1200, Vector Laboratories, Inc.).

Jara C., Cerpa W., Tapia-Rojas C, & Quintanilla R.A. (2021). Tau Deletion Prevents Cognitive Impairment and Mitochondrial Dysfunction Age Associated by a Mechanism Dependent on Cyclophilin-D. Frontiers in Neuroscience, 14, 586710.

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