Co-cultures at a 5:1 (T cells or Jurkat: silencing or overexpressing PGC-1α in NPC cells) ratio were incubated for 48 h and the Annexin V PE/7-AAD apoptosis detection Kit (FXP027, 4A Biotech) was used to measure apoptosis.
Annexin 5 pe 7 aad apoptosis detection kit
Manufactured by 4A Biotech
The Annexin V PE/7-AAD apoptosis detection Kit is a laboratory instrument used to detect and quantify apoptosis in cell samples. It combines Annexin V, a protein that binds to phosphatidylserine, and 7-AAD, a fluorescent dye that stains DNA, to identify early and late stages of apoptosis.
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Lab products found in correlation
2 protocols using annexin 5 pe 7 aad apoptosis detection kit
1
PGC-1α Regulation Impacts Apoptosis
2
Apoptosis Analysis of Keloid Fibroblasts
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Stable keloid fibroblasts were suspended and washed twice with PBS. An Annexin V PE/7-AAD apoptosis detection kit (4A Biotech) was used to treat the collected cells according to the manufacturer’s instructions, and the stained cells were analysed with a Gallios flow cytometer (Beckman Coulter).
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