Sw982

The human synovial sarcoma cell line (SW-982) was purchased from the American Type Culture Collection (ATCC). The human skin fibroblast cell line (HSF) with related media were purchased from Fenghui Biotechnology Co., Ltd (Hunan, China). The primary human synovial sarcoma cells (hSS-005R) were also established for the validation of PRGs. The human synovial sarcoma cells SW-982 and hSS-005R were cultured in Dulbecco’s modified Eagle medium (DMEM) (Gibco, United States) supplemented with 10% fetal bovine serum (FBS) (Gibco, United States) and 1% penicillin-streptomycin (NCM Biotech, China). Cells were cultured at 37°C with 5% CO₂ in a humidified incubator (Thermo Fisher Scientific, United States).

Human skeletal muscle cells (HSKMC) and Rhabdomyosarcoma cells (RH30) were purchased from American type culture collection (ATCC, U.S.A.). HSKMC were cultured in Mesenchymal Stem Cell Basal Medium with Primary Skeletal Muscle Cell Growth Kit (ATCC, U.S.A.) at 37°C in a humidified atmosphere containing 5% CO2. RH30 were cultured in were cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, U.S.A.) and 1% penicillin-streptomycin at 37 °C in a humidified atmosphere containing 5% CO₂. Synovial sarcoma cells (SW982) were purchased from FUXIANG Biotechnology (Shanghai, China). SW982 were cultured in were cultured in Dulbecco’s Modified Eagle Medium with 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, U.S.A.) and 1% penicillin-streptomycin at 37 °C in a humidified atmosphere containing 5% CO₂.

The human skin fibroblast cell line (HSF) along with their culture media were obtained from Fenghui Biotechnology Co., Ltd (Hunan, China). The human synovial sarcoma cell line (SW-982) was provided by the American Type Culture Collection (ATCC). The human liposarcoma cell line (SW-872) was purchased from Procell Life Science&Technology Co., Ltd. The primary human synovial sarcoma cells (hSS-005R) were established for the validation of NRGs. SW-982, SW-872, and hSS-005R were cultured in Dulbecco’s modified Eagle medium (DMEM) (Gibco, United States), which was supplemented with 1% penicillin-streptomycin (NCM Biotech, China) and 10% fetal bovine serum (FBS) (Gibco, United States). Cells were cultured in a humidified incubator (Thermo Fisher Scientific, United States) at 37°C and 5% CO₂.

The STS cell lines SW982 (NY-ESO-1+ HLA-A2+) and Fuji (NY-ESO-1- HLA-A2+) were kindly provided by Prof. Hiroshi Shiku (Mie University, Tsu, Japan). SW982 cells were cultured in DMEM and Fuji cells were cultured in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum (FBS) (all Thermo Fisher Scientific, Waltham, MA, USA).

The human fibrosarcoma cell line HT-1080, synovial sarcoma cell line (SW-982) and human skin fibroblast cell line (HSF) were purchased from iCell Bioscience Inc (Shanghai, China). HT-1080 cell was cultured in minimum essential medium (iCell Bioscience Inc, Shanghai, China) supplemented with 50µg/mL streptomycin (Solarbio, Beijing, China), 50U/mL penicillin (Solarbio, Beijing, China), and 10% fetal bovine serum (FBS) (Biological Industries, Israel). SW-982 cell was cultured in Leibovitz’s L15 (iCell Bioscience Inc, Shanghai, China) supplemented with 50µg/mL streptomycin (Solarbio, Beijing, China), 50U/mL penicillin (Solarbio, Beijing, China), and 10% FBS (Biological Industries, Israel). HSF cells was cultured in complete growth medium for primary fibroblast (iCell-0051a-001b, Shanghai, China). HT-1080 and HSF cells were cultured with 37°C in a humidified 5% CO₂ incubator (ThermoFisher Scientific, USA), while the SW-982 cells was cultured with 37°C without CO₂ incubotor (ThermoFisher Scientific, USA).