Eg1150h c

After successful modelling for 24 h, all the goats were fixed on an operating table and sacrificed by bloodletting through the cervical artery. After open the thoracic cavity, the lung was obtained. To ensure the consistency and validation of the samples, lung tissue blocks of the same size and same location in the middle lobe of the right lung were taken and stored in liquid nitrogen for RNA-seq analysis. And another piece of lung tissue in the middle lobe was fixed in 10% (v/v) formaldehyde for one week at room temperature and dehydrated using the Fully Enclosed Tissue Processor (ASP300S, Leica Biosystems, Germany) before embedding in paraffin (EG1150H  +  C, Leica Biosystems, Germany). The fixed paraffin lung tissues were cut into 5 μm-thick sections using the microtome (RM2245, Leica Biosystems, Germany) and then stained with H&E Staining System (ST5020, Leica Biosystems, Germany) according to the instructions. The sections were visualized with a light microscope (DM4000B, Leica Microsystems, Germany).

The rats were subjected to intraperitoneal injection of pentobarbital at a dose of 30 mg/kg for anesthesia. The goats were euthanized through exsanguination via the carotid artery. Subsequently, the lungs were collected and fixed in a 4% (w/v) formaldehyde solution for 1 week. Dehydration of the lung tissues was performed using a fully enclosed tissue processor (ASP300 S; Leica), followed by embedding them in paraffin (EG1150H + C; Leica Biosystems). For staining the preserved paraffin lung tissue sections, the H&E Staining System (ST5020; Leica) was employed. The sections, sliced to a thickness of 5 μm using a microtome (RM2245; Leica), were then examined using a light microscope (DM4000B; Leica).