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Diogenes system

Manufactured by National Diagnostics
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Sourced in United States

The Diogenes System is a high-performance laboratory equipment designed for advanced diagnostic analysis. It provides automated sample handling, processing, and analysis capabilities to support various medical and scientific applications. The core function of the Diogenes System is to facilitate efficient and accurate data collection and processing for researchers and healthcare professionals.

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Lab products found in correlation

Infinite m1000 pro, by Tecan (3 mentions) Diogenes complete enhancer solution, by National Diagnostics (2 mentions)

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Diogenes (5 citations)

3 protocols using diogenes system

1

Superoxide Production Measurement in BAECs

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The Diogenes System (National Diagnostics, Atlanta, GA, USA) is a superoxide (O2•−) chemiluminescence enhancer that was used to measure O2•− production. BAECs were seeded in a flat white-bottom 96-well plate at a concentration of 4–6 × 104 cells per well. Cells were allowed to attach overnight. One day before the experiment, cells were washed once with PBS and media was changed overnight to phenol-free media including penicillin (100 U/mL), streptomycin (100 μg/L), and L-glutamine (2 mM). On the day of the experiment, cells were treated with 100 μL of Diogenes Complete Enhancer Solution (National Diagnostics) and either sildenafil (5 μM) or placebo was added. Cells were subsequently irradiated with 10 Gy and the light output was read on a luminescent microplate reader (Infinite M1000 Pro; Tecan Group, Männedorf, Switzerland) for up to 20 minutes. Xanthine (100 μL of 0.5 mM in 50 mM potassium phosphate buffer)/xanthine oxidase (20 μL of 10−3 units per mL in 50 mM potassium phosphate buffer) solution was added as a positive control according to the manufacturer’s instructions.
Wortel R.C., Mizrachi A., Li H., Markovsky E., Enyedi B., Jacobi J., Brodsky O., Cao J., Lippert A.R., Incrocci L., Mulhall J.P, & Haimovitz-Friedman A. (2019). Sildenafil Protects Endothelial Cells From Radiation-Induced Oxidative Stress. The journal of sexual medicine, 16(11), 1721-1733.
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2

Superoxide Production Measurement in BAECs

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Diogenes System (National Diagnostics, Atlanta, GA, USA) is a superoxide (O2•−) chemiluminescence enhancer that was used to measure O2•− production. BAECs were seeded in a flat white-bottom 96-well plate at a concentration of 4–6 × 104 cells per well. Cells were allowed to attach overnight. One day before the experiment, cells were washed once with PBS and media was changed overnight to phenol-free media including penicillin (100 U/mL), streptomycin (100 μg/L), and L-glutamine (2 mM). On the day of the experiment, cells were treated with 100 μL of Diogenes Complete Enhancer Solution (National Diagnostics) and either sildenafil (5 μM) or placebo was added. Cells were subsequently irradiated with 10 Gy and the light output was read on a luminescent microplate reader (Infinite M1000 Pro; Tecan Group, Männedorf, Switzerland) for up to 20 minutes. Xanthine (100 μL of 0.5 mM in 50 mM potassium phosphate buffer)/xanthine oxidase (20 μL of 10−3 units per mL in 50 mM potassium phosphate buffer) solution was added as a positive control according to the manufacturer’s instructions.
Wortel R.C., Mizrachi A., Li H., Markovsky E., Enyedi B., Jacobi J., Brodsky O., Cao J., Lippert A.R., Incrocci L., Mulhall J.P, & Haimovitz-Friedman A. (2019). Sildenafil Protects Endothelial Cells From Radiation-Induced Oxidative Stress. The journal of sexual medicine, 16(11), 1721-1733.
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3

Measuring Superoxide Production in BAEC

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Diogenes System (National Diagnostics, USA) is a superoxide (O2•−) chemiluminescence enhancer that was used to measure O2•− production. BAEC cells were seeded in a flat white-bottom 96 well at a concentration of 4–6×104 cells per well. Cells were allowed to attach overnight. One day prior to the experiment cells were washed once with PBS and media was changed overnight to phenol-free media containing penicillin (100U/ml), streptomycin (100μg/l), and L-glutamine (2mM). On the day of the experiment cells were treated with 100μL of Diogenes Complete Enhancer Solution and DOX or CIS, and the light output was read on a luminescent microplate reader (Infinite M1000 Pro, Tecan, Swiss) for up to 20 minutes. Xanthine (100μL of 0.5mM in 50mM potassium phosphaste buffer)/ Xanthine Oxidase (20μL of 10−3 units per mL in 50mM potassium phosphaste buffer) solution was added as a positive control according to the manufacturer’s instructions.
Mizrachi A., Ben-Aharon I., Li H., Bar-Joseph H., Bodden C., Hikri E., Popovtzer A., Shalgi R, & Haimovitz-Friedman A. (2021). Chemotherapy-induced acute vascular injury involves intracellular generation of ROS via activation of the acid sphingomyelinase pathway. Cellular signalling, 82, 109969.
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