Trastuzumab was kindly provided by Robert Ivkov, PhD (The Johns Hopkins University School of Medicine), and was used after purification. Paclitaxel was purchased from LKT Laboratories, Inc. The amine-reactive TCO-NHS ester and Tetrazine-Peg4-NHS ester were purchased from Sigma-Aldrich Corp, and Kerafast, Inc., respectively. NHS-Alexa Fluor® 488 was purchased from Invitrogen Corp. Dry or HPLC-grade solvents were purchased from Sigma-Aldrich Corp. and used without further purification. XenoLight CF 680 and CF 750 NIR fluorescent dyes were purchased from PerkinElmer, Inc. NHS-Rhodamine and NHS-DyLight 800 dyes were purchased from Life Technologies Corp.
Paclitaxel
Manufactured by LKT Laboratories
Sourced in United States
Paclitaxel is a laboratory reagent produced by LKT Laboratories. It is a naturally occurring compound isolated from the bark of the Pacific yew tree. Paclitaxel inhibits cell division by stabilizing microtubules, which are essential components of the cellular cytoskeleton.
Automatically generated - may contain errors
Lab products found in correlation
Nhs alexafluor488, by Thermo Fisher Scientific (1 mentions)
Nhs rhodamine, by Thermo Fisher Scientific (1 mentions)
Xenolight cf 680, by PerkinElmer (1 mentions)
Ammonium pyrrolidinedithiocarbamate pdtc, by Merck Group (1 mentions)
D lys3 ghrp 6, by Bio-Techne (1 mentions)
Mevalonolactone, by Merck Group (1 mentions)
Lovastatin, by Merck Group (1 mentions)
Doxorubicin, by Thermo Fisher Scientific (1 mentions)
Fluvastatin, by Selleck Chemicals (1 mentions)
Pravastatin, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Topotecan, by LGC (1 mentions)
Atorvastatin, by LGC (1 mentions)
Simvastatin, by Merck Group (1 mentions)
Ly294002, by Cell Signaling Technology (1 mentions)
Ishikawa, by Merck Group (1 mentions)
Sulforaphane, by LKT Laboratories (1 mentions)
Torin 2, by Cell Signaling Technology (1 mentions)
Topotecan, by Selleck Chemicals (1 mentions)
Tunicamycin, by Cayman Chemical (1 mentions)
5 protocols using paclitaxel
1
Trastuzumab-Paclitaxel Nanoconjugate Synthesis
2
Inhibition of NF-κB and Ghrelin Signaling in Paclitaxel-Treated Rats
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Paclitaxel (LKT Laboratories inc, St. Paul, MN, USA) was dissolved in 10% dimethyl sulfoxide (10%DMSO). Ammonium pyrrolidine dithiocarbamate (PDTC; Sigma-Aldrich, St Louis, MO, USA), an NFκB inhibitor, and [D-Lys3]-GHRP-6 (Tocris, Bristol, United Kingdom), a ghrelin receptor antagonist, were dissolved in saline. RKT (Tsumura, Tokyo, Japan) was dissolved in distilled water (DW).
Paclitaxel was injected intraperitoneally (i.p.) at a dose of 2 or 5 mg/kg. PDTC was injected intrathecally (i.t.) at a dose of 100 ng/5μL. [D-Lys3]-GHRP-6 was injected i.p. at a dose of 10 mg/kg. RKT was administered orally (p.o.) at a dose of 0.1, 0.3, or 1 g/kg. Control animals were injected with the respective vehicle for each drug.
Paclitaxel was injected intraperitoneally (i.p.) at a dose of 2 or 5 mg/kg. PDTC was injected intrathecally (i.t.) at a dose of 100 ng/5μL. [D-Lys3]-GHRP-6 was injected i.p. at a dose of 10 mg/kg. RKT was administered orally (p.o.) at a dose of 0.1, 0.3, or 1 g/kg. Control animals were injected with the respective vehicle for each drug.
3
Synergistic Chemo-Statin Combination Protocol
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The chemotherapeutic agents used included doxorubicin (Fisher Scientific, Waltham, MA, USA), paclitaxel (LKT Laboratories Inc.. St. Paul, MN, USA) and topotecan (Toronto Research Chemicals, Toronto, Canada). The statin drugs used in the research include atorvastatin (Toronto Research Chemicals Inc., Toronto, Canada), fluvastatin (Selleck Chemicals, Houston, TX, USA), lovastatin (EMD Millipore, Billerica, MA, USA), mevastatin (EMD Millipore, Billerica, MA, USA), pravastatin (EMD Millipore, Billerica, MA, USA) and simvastatin (EMD Millipore, Billerica, MA, USA). Mevalonolactone (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in RPMI-1640 medium (Fisher Scientific, Waltham, MA, USA) and titrated with NaOH to a pH of 7.8 to form mevalonate. doxorubicin, fluvastatin and pravastatin were dissolved in distilled water. paclitaxel, topotecan, and atorvastatin were dissolved in methanol. lovastatin, mevastatin and simvastatin were dissolved in dimethyl sulfoxide (DMSO). The chemotherapeutic agents, statins, and mevalonate were aliquoted and stored at − 80 °C.
4
Endometrial Cancer Cell Lines: Authentication and Culture
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AN3CA, KLE, Hec1A, Hec1B, MFE280, and MFE296 were obtained as a gift from the lab of Jie Wu Ph.D., University of Oklahoma Health Sciences Center, Oklahoma city, OK, 73104, USA. Ishikawa was purchased from Sigma (St Louis, MO, USA). AN3CA, KLE, Hec1B, MFE280, and MFE296 were authenticated by short tandem repeat (STR) profiling at the University of Arizona Genetics Core in 2017 and passaged less than 20 times since authentication. AN3CA, Ishikawa, and Hec-1B were grown in EMEM, MFE280, and MFE296 were grown in DMEM, KLE was grown in DMEM/F12, and Hec1A was grown in McCoy’s 5a. All media was supplemented with 10% FBS and 1% penicillin/streptomycin. Sulforaphane (#S8044; Cas No. 4478-93-7) and paclitaxel (#P0093; Cas No. 33069-62-4) were obtained from LKT laboratories (St. Paul, MN, USA). PI3K inhibitor, LY294002 (#9901), mTOR inhibitor, Torin2 (#14385), and ERK inhibitor U0126 (#9903) were purchased from Cell Signaling Technology (Danvers, MA, USA). In all of the experiments, cells treated with vehicle only were considered as the control and all treatments were given in medium supplemented with 10% FBS.
5
Drug Screening and Cellular Assay Protocol
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