Streptomycin glutamate

DRG cell lines (ND 7/23) and mouse microglial cells (SIM-A9) were obtained from Sigma-Aldrich (#92090903, USA) and Kerafast (END001, USA), respectively. The ND 7/23 cells were grown in Dulbecco's Modified Eagle Medium (DMEM, #12100046; Gibco, USA) and the SIM-A9 cells were maintained in DMEM/F12 (#11320033; Gibco, USA) supplemented with 10% FBS, 100 U/ml of penicillin, and 100 μg/ml of streptomycin-glutamate (Thermo Scientific, Utah, USA). Both cell lines were maintained in a 5% CO₂ incubator (humidified atmosphere) at 37 °C.

SiHa parental cells were from ATCC and cultured in DMEM medium (Thermo Scientific, Utah, USA, SH30003.02) supplemented with 5% fetal bovine serum (Thermo Scientific, Utah, USA, SH30071.03), 100U/mL of penicillin, and 100μg/mL of streptomycin–glutamate (Thermo Scientific, Utah, USA, 10378016) in a 5% CO₂ incubator (humidified atmosphere) at 37°C. S3 cells, an oxaliplatin-resistant subclone of SiHa cells, were cultured in DMEM medium supplemented with 10% fetal bovine serum, 100 U/mL of penicillin, 100 μg/mL of streptomycin–glutamate, and 2 μg/mL of oxaliplatin. For the following assays, resistant cells were cultured in the medium without oxaliplatin to prevent the interference caused by oxaliplatin.