Thermo genesys 10s

In this work, UV absorbance spectra of the naproxen acid, naproxen sodium salt, and pharmaceutical preparations containing NAS (NA1-NA4) and NSS (NS1-NS5) as the active pharmaceutical ingredients were registered. For this purpose, 10 mg of each of the samples were dissolved in 100 mL of 96% methyl alcohol (Sigma-Aldrich Company, St. Louis, MO, USA). The samples were then mixed and poured into a quartz cuvette placed in a UV-Vis spectrophotometer. UV absorbance spectra were obtained in the wavelength range from 245 nm to 345 nm. The UV-Vis spectrophotometer Thermo Genesys 10S produced by Thermo Scientific (Waltham, MA, USA) was used. To obtain and analyze UV spectra, VisionLite Software (Thermo Scientific Company, Waltham, MA, USA) and Origin 2016 (OriginLab Company, Northampton, MA, USA) were used.

UV spectra of the pure substances theophylline, aminophylline, ethylenediamine hydrochloride, and pharmaceutical preparations Euphyllin^® long 200 mg, and 300 mg (zr pharma&GmbH company, Wien, Austria), Theospirex^® retard 150 mg, and 300 mg (BIOFARM company, Poznan, Poland), Aminophylline 100 mg and 200 mg (advacare pharma, Cheyenne, WY, USA) were analyzed in the study. For this purpose, 1 mg of each of the tested samples was dissolved in 100 mL of 96% ethyl alcohol (POCh company, Gliwice, Poland). The samples were then mixed and poured into a quartz cuvette, which was then placed in a spectrophotometer. UV absorbance spectra were recorded in the wavelength range of 200–380 nm. UV-Vis spectrophotometer Thermo Genesys 10S produced by Thermo Scientific (Waltham, MA, USA) was used. The analysis of obtained UV spectra was performed using the programs VisionLite produced by Thermo Scientific (Waltham, MA, USA) and Origin 2016 produced by OriginLab (Northampton, MA, USA).

The ABTS radical was produced by a reaction of 7 mM ABTS with 2.45 mM potassium persulfate in the dark at room temperature (25 °C). The mixture was allowed to stand for 16 h before use. The aqueous ABTS⁺ solution was diluted with water to obtain an absorbance of 0.7 at 734 nm. Subsequently, 3 mL of ABTS⁺ solution was added to 30 μL of the extracts [25 (link)]. After 6 min, absorbance was measured at 734 nm using a Thermo Genesys 10S spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Trolox was used as a standard. Analyses were performed in triplicates, and the results were expressed as mmol TE/g extract.