2 7 dichlorodihydrofluorescin dchf

Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Gibco (USA). Fetal bovine serum (FBS) was from Hyclone Lab Inc. (USA). Sulforhodamine B (SRB), Hoechst 33,258, 2′,7′-dichlorodihydrofluorescin (DCHF) and JC-1 were from Sigma Co. (USA). Primary antibodies against β-actin, PI3K and p-PI3K were from Santa Cruz Biotechnology (USA). Primary antibody against caspase 3, PARP, LC3B, p70S6K, p-p70S6K, p-mTOR, mTOR, Akt, p-Akt, p38 MAPK, p-p38 MAPK and secondary antibody (horseradish peroxidase) were from Cell Signaling Technology (USA). Primary antibody against p62 was from BD Transduction Laboratories. Primary antibody against LOX-1 was from ABclonal (USA). Wortmannin was obtained from Selleck (USA). Ox-LDL was purchased from Beijing Xiesheng Biotechnology (China). All other reagents were ultrapure grade.

DMEM was obtained from Gibco BRL Co. (USA). Fetal bovine serum was from Hyclone Laboratories Inc. (USA). Acridine orange (AO) was from Amresco (USA). Hoechst 33258, 2′,7′-dichlorodihydrofluorescin (DCHF), JC-1, propidium iodide (PI), and sulforhodamine B (SRB) were from Sigma Co. (USA). The antibodies against p53, ANXA7, NF-κB p65, β-actin, and horseradish peroxidase-conjugated secondary antibodies were from Santa Cruz Biotechnology (USA). Secondary antibodies for immunofluorescence, donkey anti-rabbit IgG Alexa Fluor-488, were purchased from Life Technologies (USA). All the other reagents were of ultrapure grade.

Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from Gibco (USA). Sulforhodamine B (SRB), acridine orange (AO), Hoechst 33258, 2′,7′-dichlorodihydrofluorescin (DCHF), and JC-1 were from Sigma Co. (USA). Primary antibodies against β-actin, PI3K, and p-PI3K were from Santa Cruz Biotechnology (USA). Primary antibodies against caspase 3, PARP, LC3B, p70S6K, p-p70S6K, p-mTOR, mTOR, Akt, p-Akt, Beclin 1, Atg7, and Nrf2 and secondary antibody (horseradish peroxidase) were from Cell Signaling Technology (USA). Primary antibodies against Bcl-2 and Bax were from ABclonal biotech (USA). Primary antibody against p62 was from BD Transduction Laboratories (USA). Primary antibody against HO-1 was from Abcam (USA). Primary antibody Lamin B was from Beyotime (China). Secondary antibody for immunofluorescence, donkey anti-rabbit IgG Alexa Fluor-488, was purchased from Life Technologies (USA). Wortmannin was obtained from Selleck (USA). Ox-LDL was purchased from Beijing Xiesheng Biotechnology (China). DPPH, ABTS, α-tocopherol (Vitamin E), gallic acid, quercetin, and the standards used in HPLC analysis were purchased from Sigma (St. Louis, MO, USA). All other reagents were of ultrapure grade.