H1387

Manufactured by Merck Group

Sourced in United States

H1387 is a laboratory equipment product manufactured by Merck Group. It serves as a general-purpose device for various applications in scientific research and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Triton x 100, by Merck Group (2 mentions) Ecl chemiluminescence, by Thermo Fisher Scientific (2 mentions) Image lab 4, by Bio-Rad (2 mentions) Bp152 5, by Thermo Fisher Scientific (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Nitrocellulose, by Bio-Rad (2 mentions) Bp120 1, by Merck Group (2 mentions) E3889, by Merck Group (2 mentions) S6508, by Roche (2 mentions) L3771, by IBI Scientific (2 mentions) Ib74040, by Merck Group (2 mentions) G5516, by Merck Group (1 mentions) Villin, by Santa Cruz Biotechnology (1 mentions) H3375, by Merck Group (1 mentions) Dithiothreitol (dtt), by Avantor (1 mentions) Ab53554, by Abcam (1 mentions)

4 protocols using h1387

Mouse Intestinal Mucosal Cell Isolation and Analysis

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Mouse intestinal mucosal cells were collected by scraping from mouse colon,
including proximal and distal colon, as previously described. ¹⁰ Briefly, mouse mucosal cells were lysed in lysis buffer
(1% Triton X-100 (Sigma-Aldrich, X100), 150 mM NaCl (J.T. Baker 3624-19), 10 mM
Tris (Fisher Scientific, BP152-5) pH 7.4, 1 mM EDTA (Fisher Scientific, BP120-1), 1 mM
EGTA (Sigma-Aldrich, E3889) pH 8.0, 0.2 mM sodium ortho-vanadate (Sigma-Aldrich, S6508)
and protease inhibitor cocktail (Roche Diagnostics, 118367001). Cultured cells were rinsed
twice in ice-cold Hanks’ balanced salt solution (Sigma-Aldrich, H1387), lysed in
protein loading buffer (50 mM Tris, pH 6.8, 100 mM dithiothreitol (Amresco, 0281),
2% SDS (Sigma-Aldrich, L3771), 0.1% bromophenol blue (IBI Scientific,
IB74040) and 10% glycerol (Sigma-Aldrich, G5516)) and sonicated (Branson Sonifier,
250). Equal amount of protein was separated by SDS-polyacrylamide gel electrophoresis,
transferred to nitrocellulose (Bio-rad, 162-0112) and immunoblotted with primary
antibodies: Villin (Santa Cruz, sc7672), ZO-1 (Invitrogen, 33–9100) or
β-actin (Sigma-Aldrich, A1978) antibodies and visualized by ECL chemiluminescence
(Thermo Scientific, 32106). Membranes probed with more than one antibody were stripped
before re-probing. Western blot bands were quantified using Image Lab 4.01 (Bio-Rad).

Zhang Y.G., Wu S., Yi J., Xia Y., Jin D., Zhou J, & Sun J. (2017). Target intestinal microbiota to alleviate disease progression in amyotrophic lateral sclerosis. Clinical therapeutics, 39(2), 322-336.

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Biodegradation Behavior of Iron Samples

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Two different kinds of degradation test, static immersion and potentiodynamic polarization tests were used to assess the biodegradation behavior of the iron samples in a modified Hanks’ solution having ionic composition and concentration close to that of human blood plasma. The test solution was prepared by dissolving Hanks’ modified salt (H1387, Sigma Aldrich, USA) in deionized water, with the addition of HEPES acid (H3375, Sigma Aldrich, Canada); sodium bicarbonate (SX0320-1, Merck KGaA, Germany) and HEPES sodium salt (DB0265, Bio Basic, Canada) as buffers to adjust the pH of the solution to 7.4, the pH of human blood plasma. The composition of Hanks’ solution was presented in Ref. (31). The static immersion and potentiodynamic polarization tests were performed following ASTM G31³² (link) and G59³³ (link) standards, respectively.

Obayi C.S., Tolouei R., Mostavan A., Paternoster C., Turgeon S., Okorie B.A., Obikwelu D.O, & Mantovani D. (2014). Effect of grain sizes on mechanical properties and biodegradation behavior of pure iron for cardiovascular stent application. Biomatter, 6(1), e959874.

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Immersion Testing of Zinc Alloys

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The immersion test on the as-cast pure Zn and Zn alloy buttons (Ø16 × 3 mm) were conducted in Hank's solution (HBSS, Sigma-Aldrich H1387) at 37 °C according to ASTM F3263-18a [43 (link)]. The solution volume to sample surface area ratio was controlled at 0.2 mL/mm². The specimens were collected at the 4-week and 8-week immersion periods. The specimens were rinsed in ethanol, then dried with compressed air for corrosion morphology characterization (SEM). The corrosion products of the specimens were removed by chromic oxide solution (200 g/L Cr₂O₃) at 40 °C for 5 min. The acid cleaning was conducted multiple times until the weight of the samples was stabilized.
The corrosion rate of the test specimen was calculated by equation:

Equation 1.

where K is a constant; W is the weight loss of the specimen in g; A, t, ρ are the surface area (cm²), time of exposure (h), and the density of the specimen (g/cm³), respectively.

Yang N., Balasubramani N., Venezuela J., Almathami S., Wen C, & Dargusch M. (2020). The influence of Ca and Cu additions on the microstructure, mechanical and degradation properties of Zn–Ca–Cu alloys for absorbable wound closure device applications. Bioactive Materials, 6(5), 1436-1451.

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Isolation and Analysis of Mouse Intestinal Mucosal Cells

Cited 1 time

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Mouse intestinal mucosal cells were collected by scraping from mouse colon, including proximal and distal colon, as previously described.^{28 (link)} Briefly, mouse mucosal cells were lysed in lysis buffer (1% Triton X-100 (Sigma-Aldrich, X100), 150 mM NaCl (J.T. Baker 3624–19), 10 mM Tris (Fisher Scientific, BP152-5) pH 7.4, 1 mM EDTA (Fisher Scientific, BP120-1), 1 mM EGTA (Sigma-Aldrich, E3889) pH 8.0, 0.2 mM sodium ortho-vanadate (Sigma-Aldrich, S6508) and protease inhibitor cocktail (Roche Diagnostics, 118,367,001). Cultured cells were rinsed twice in ice-cold Hanks’ balanced salt solution (Sigma-Aldrich, H1387), lysed in protein loading buffer (50 mM Tris, pH 6.8, 100 mM dithiothreitol (Amresco, 0281), 2% SDS (Sigma-Aldrich, L3771), 0.1% bromophenol blue (IBI Scientific, IB74040) and 10% glycerol (Sigma-Aldrich, G5516)) and sonicated (Branson Sonifier, 250). Equal amount of protein was separated by SDS-polyacrylamide gel electrophoresis, transferred to nitrocellulose (Bio-rad, 162–0112) and immunoblotted with primary antibodies: anti-GFAP (Abcam, ab53554), SMMHC (Abcam, ab53219), human SOD-1⁵⁸ or β-actin (Sigma-Aldrich, A1978) antibodies and visualized by ECL chemiluminescence (Thermo Scientific, 32,106). Membranes probed with more than one antibody were stripped before re-probing. Western blot bands were quantified using Image Lab 4.01 (Bio-Rad).

Zhang Y., Ogbu D., Garrett S., Xia Y, & Sun J. (2021). Aberrant enteric neuromuscular system and dysbiosis in amyotrophic lateral sclerosis. Gut Microbes, 13(1), 1996848.

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