For the observation of epicuticular wax crystal, the samples were flattened and allowed to dry under 50–60 °C. About 0.5 cm segments were mounted onto scanning electron microscopy (SEM) stubs, and then coated with gold particles in an ion sputter coater (Hitachi E-1045, Japan). The coated samples were viewed with a Hitachi S-4800 field emission SEM using an accelerating voltage of 5 kV.
S 4800 field
Manufactured by Hitachi
Sourced in Japan
The S-4800 field emission scanning electron microscope (FE-SEM) is an advanced imaging tool designed by Hitachi for high-resolution, high-magnification analysis of materials. It utilizes a field emission electron source to produce a small, high-brightness electron beam, enabling the capture of detailed images with exceptional resolution and clarity.
Automatically generated - may contain errors
Lab products found in correlation
E 1045, by Hitachi (1 mentions)
K550x sputter coater, by Quorum Technologies (1 mentions)
Zno nps, by Hitachi (1 mentions)
S 4800 fesem, by Hitachi (1 mentions)
Ultima 4, by Rigaku (1 mentions)
Cary 5000, by Agilent Technologies (1 mentions)
X maxn 80 tle, by Oxford Instruments (1 mentions)
Arm200f, by JEOL (1 mentions)
670 ir spectrometer, by Agilent Technologies (1 mentions)
Freezone 4.5 l freeze dry system, by Labconco (1 mentions)
208hr high resolution sputter coater, by Cressington (1 mentions)
11 protocols using s 4800 field
1
Epicuticular Wax Crystal Observation
2
Scanning Electron Microscopy of Powder Samples
3
Characterization of ZnO Nanoparticles
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ZnO NPs were purchased from Shanghai Aladdin Reagent Co., Ltd, with a purity of 99.5 % and particle size of 30± 10 nm provided by the producer. The size of ZnO NPs measured in our laboratory using a Hitachi S-4800 Field Emission Scanning Electron Microscope (Hitachi S-4800 FE-SEM) was 30±12 nm (Supplementary Material Fig. S1). X-ray difffaction analysis (XRD, Rigaku Ultima IV) of the nanoparticles revealed a pure ZnO minerals composed of zincite (Supplementary Material Fig. S2). The specific surface area determined using the multi-point Brunauer-Emmett-Teller (BET) method was 42.0 m 2 g -1 . All other chemicals of analytical reagent grade were purchased from Sinopharm Chemical Regent Co., Ltd, China.
4
Characterization of Porous Nanocomposite Materials
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SEM samples were examined using a Hitachi S-4800 field-emission scanning electron microscope (FE-SEM). High-resolution TEM samples were investigated using a high resolution transmission electron microscope (HR-TEM, STEM, JEOL ARM-200F) having a probe Cs aberration corrector (CEOS GmbH) and an energy-dispersive X-ray spectroscopy (EDS, Oxford Instruments X-MaxN 80 TLE) attached to the TEM. The porous structures of the samples were analyzed in a nitrogen adsorption experiment at −196°C using a BEL BELSORP-Max system. The surface areas and pore sizes of the samples were calculated using the Brunauer-Emmett-Teller (BET) equation and the Barrett-Joyner-Halenda (BJH) method, respectively. Infrared spectra were recorded by a Varian 670-IR spectrometer equipped with an attenuated total reflectance (ATR) device. Nuclear magnetic resonance (NMR) spectra were recorded by an Agilent 400 MHz spectrophotometer using CDCl3 as the solvent and tetramethylsilane (TMS) as the internal standard. A thermal analysis was performed at a heating rate of 5°C min−1 in a nitrogen atmosphere using a thermogravimetric analyzer (TGA, SDT Q600). Isothermal analysis was performed at 250°C. The dissolution of PQ and PQ/CMK-3 in TEGDME was investigated by ultraviolet-visible (UV-VIS) spectroscopy (Varian, Cary 5000).
5
Characterizing Carrageenase Immobilization by FTIR and SEM
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To obtain FTIR spectra in the 4,000-400 cm-1 region, we turned to a NEXUS 670 FTIR instrument using KBr discs at room temperature (25°C). The morphology of the immobilized carrageenase was revealed by SEM (Hitachi S-4800 Field, Japan).
6
Nanoparticle Imaging via SEM
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Particles were diluted 1:100 in filtered distilled water. An aliquot of 0.5 mL was vacuum‐filtered onto a 0.2 µm polycarbonate filter, and the filter was affixed onto a 13‐mm aluminum stub mount using double stick carbon tape. The mounted filter was then sputter‐coated with gold‐palladium for 2 minutes. The particles were imaged using a Hitachi S4800 field‐emission scanning electron microscope at 5 kV.
7
Biofilm Cell Harvesting and Imaging
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Biofilm cells were harvested after 24-h fermentation in the presence of SNP and control. Samples were washed twice with PBS buffer, and stored at − 80◦C. Biofilm cells were dried using a FreeZone® 4.5 L Freeze Dry System (Labconco, KansasCity, MO, USA) and sputter coated with gold. Images were obtained using a Hitachi S-4800 field.
8
Cryogenic Fracture Surface Imaging
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Liquid nitrogen was used to fracture the studied samples. The fractured surfaces were investigated by SEM using the Hitachi S-4800 field-emission scanning electron microscope at different magnifications. An accelerating voltage of 10 kV and a working distance of ca. 9 to 10 mm were applied.
9
Scanning Electron Microscopy of Bivalve Shells
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Scanning electron microscopy (SEM) was used to determine fine-scale shell valves features for the taxonomic description and the presence of bacteria associated with the gill. For SEM imaging of soft tissues and hard parts, samples were dissected, critical point dried using the Samdri PVT-3D critical point dryer (Tousumis, Maryland, USA), mounted on a standard aluminium SEM stub and coated with platinum to a thickness of 5 nm using the Cressington 208 HR High Resolution Sputter Coater (Cressington Scientific Instruments, Hertfordshire, UK). Samples were imaged on the Hitachi S-4800 field emission scanning electron microscope. Gill tissues were manually fractured using a razor blade prior to coating to expose bacterial symbionts.
10
3D-Printed Hydrogel Surface Characterization
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Surface contact angle measurements were taken using a Ramé-Hart Model 290 F1 Advanced Goniometer on untreated samples, plasma-treated samples, and plasma + PEG-treated samples. Initial as well as receding contact angle measurements were carried out at ambient temperature by placing a ∼10 μL water droplet onto the 3D printed surface before and after the different surface modifications.
The microstructure network of the produced hydrogel and its ability to capture bacteria within its porous matrix was evaluated using Hitachi S-4800 field emission scanning electron microscopy (SEM) after Au/Pd sputtering. SEM imaging was performed on bare and bacteria-captured hydrogel samples. The first hydrogel sample was placed in a solution of PBS for 8 hours, while the second hydrogel was placed into a solution of 100% TSB inoculated with E. coli. Both hydrogel samples were freeze dried using a LyoStar 3 Freeze-dryer from SP Scientific for 18 hours prior to performing the SEM imaging.
The microstructure network of the produced hydrogel and its ability to capture bacteria within its porous matrix was evaluated using Hitachi S-4800 field emission scanning electron microscopy (SEM) after Au/Pd sputtering. SEM imaging was performed on bare and bacteria-captured hydrogel samples. The first hydrogel sample was placed in a solution of PBS for 8 hours, while the second hydrogel was placed into a solution of 100% TSB inoculated with E. coli. Both hydrogel samples were freeze dried using a LyoStar 3 Freeze-dryer from SP Scientific for 18 hours prior to performing the SEM imaging.
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