P stat1 9167s

Manufactured by Cell Signaling Technology

P-STAT1 (9167S) is a primary antibody that recognizes phosphorylated STAT1 (Tyr701). It is used to detect the phosphorylation of STAT1 by western blotting.

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Lab products found in correlation

Neuro 2a cells, by American Type Culture Collection (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Odyssey imaging system, by LI COR (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Cdk4 12790s, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

STAT1 (305 citations) P-STAT1 (218 citations)

2 protocols using p stat1 9167s

Neuroblastoma Cell Line Protocol

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Human (LAN-1 and SK-N-SH) NB cell lines were obtained from Dr. Dai Chung (Vanderbilt University). Neuro-2a cells were obtained from American Type Culture Collection (ATCC) (Manassas, Virginia). 9464D cells were derived from spontaneous TH-MYCN tumors70 (link) and obtained from Dr. Yonghzi Cui (National Cancer Institute). Cells were maintained in Dulbecco’s modified Eagle’s medium (GIBCO) supplemented with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, California) and 1% penicillin/streptomycin (GIBCO). Antibodies against STING (13 647S), p-IRF3 (4997S), IRF3 (4302S), Cleaved-Caspase 3 (9664T), and p-STAT1 (9167S) were purchased from Cell Signaling Technology (Beverly, Massachusetts). Antibodies for β-Actin (A5411) and Caspase (SC 56053) were purchased from Sigma Aldrich (St. Louis, Missouri) and Santa Cruz Biotechnology, respectively. Anti-PD-L1 antibody (BE0101) was purchased from BioXcell (West Lebanon, New Hampshire).

Wang-Bishop L., Wehbe M., Shae D., James J., Hacker B.C., Garland K., Chistov P.P., Rafat M., Balko J.M, & Wilson J.T. (2020). Potent STING activation stimulates immunogenic cell death to enhance antitumor immunity in neuroblastoma. Journal for Immunotherapy of Cancer, 8(1), e000282.

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Protein Expression Analysis by WB

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Protein extracts (20-30 µg/sample) were separated by 10% acrylamide gel SDS–PAGE, and immunoblotted with antibodies against p62/SQSTM1 (5114S), Cyclin D1 (5114S), pErk (2978S), CDK4 (12790S), p21 (2946S), pSTAT1 (9167S) from (Cell Signaling Technology) and pc-Jun (PAS-17879) (ThermoFisher Scientific). Actin was used as control (SC-1516, Santa Cruz Biotechnology, Santa Cruz, CA). Membranes were blocked with 5% BSA (Sigma-Aldrich) in Tris-buffered saline, 0.1% Tween 20 and, after incubation with primary antibodies, signals were visualized with IR-dye-conjugated secondary antibodies and scanned using Odyssey imaging system (Li-COR, Lincoln, NE).

Degan S., May B.L., Jin Y.J., Hammoda M.B., Sun H., Zhang G., Wang Y., Erdmann D., Warren W, & Zhang J.Y. (2022). Co-Treatment of Chloroquine and Trametinib Inhibits Melanoma Cell Proliferation and Decreases Immune Cell Infiltration. Frontiers in Oncology, 12, 782877.

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