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Pgl4.74 renilla luciferase expression vector

Manufactured by Promega
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The PGL4.74 Renilla luciferase expression vector is a plasmid DNA construct designed for the expression of the Renilla luciferase reporter gene in various cell lines. It contains the Renilla luciferase coding sequence under the control of a constitutive promoter, allowing for the production of the Renilla luciferase enzyme in transfected cells.

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Lab products found in correlation

Synergy 2, by Agilent Technologies (2 mentions) In fusion hd cloning kit, by Takara Bio (2 mentions) Dual luciferase reporter assay system, by Promega (2 mentions) X tremegene hp dna transfection reagent, by Roche (2 mentions)

Close spelling variants of this product

PGL4.74 Renilla luciferase PGL4.74 Renilla vector Renilla luciferase vector pGL4.74 Renilla expression vector pGL4.74 PGL4 luciferase expression vector Renilla luciferase expression vector PGL4.74 vector Renilla luciferase vector Renilla luciferase PGL4 vector

2 protocols using pgl4.74 renilla luciferase expression vector

1

Cloning and Functional Assay of Socs3 Regions

Check if the same lab product or an alternative is used in the 5 most similar protocols
The three genomic regions around Socs3 (Socs3–1, Socs3–2, and Socs3–3) were amplified via PCR from the mouse genome using specific primers (Supplementary Table 4), and cloned into the pGL4.23 (Promega) enhancer assay vector using In-Fusion HD cloning kit (Takara) following the manufacturer’s protocol. For luciferase assays, mHypoA-POMC/GFP-1 cells were seeded in 96-well plate with 1×104 cells/well at 24 hours before transfection. 120 ng of plasmid per well were transfected along with 30 ng of pGL4.74 Renilla luciferase expression vector (Promega), to correct for transfection efficiency, using XtremeGENE HP DNA transfection reagent (Roche). DNA:XtremeGENE ratio was 1:2. Firefly and Renilla luciferase activities were measured 24 hours after transfection using the Dual-luciferase reporter assay system (Promega) on a Synergy2 (Biotek) following the manufacturer’s protocol.
Inoue F., Eckalbar W.L., Wang Y., Murphy K.K., Matharu N., Vaisse C, & Ahituv N. (2019). Genomic and epigenomic mapping of leptin-responsive neuronal populations involved in body weight regulation. Nature metabolism, 1(4), 475-484.
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2

Cloning and Functional Assay of Socs3 Regions

Check if the same lab product or an alternative is used in the 5 most similar protocols
The three genomic regions around Socs3 (Socs3–1, Socs3–2, and Socs3–3) were amplified via PCR from the mouse genome using specific primers (Supplementary Table 4), and cloned into the pGL4.23 (Promega) enhancer assay vector using In-Fusion HD cloning kit (Takara) following the manufacturer’s protocol. For luciferase assays, mHypoA-POMC/GFP-1 cells were seeded in 96-well plate with 1×104 cells/well at 24 hours before transfection. 120 ng of plasmid per well were transfected along with 30 ng of pGL4.74 Renilla luciferase expression vector (Promega), to correct for transfection efficiency, using XtremeGENE HP DNA transfection reagent (Roche). DNA:XtremeGENE ratio was 1:2. Firefly and Renilla luciferase activities were measured 24 hours after transfection using the Dual-luciferase reporter assay system (Promega) on a Synergy2 (Biotek) following the manufacturer’s protocol.
Inoue F., Eckalbar W.L., Wang Y., Murphy K.K., Matharu N., Vaisse C, & Ahituv N. (2019). Genomic and epigenomic mapping of leptin-responsive neuronal populations involved in body weight regulation. Nature metabolism, 1(4), 475-484.
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