Total proteins were extracted with RIPA lysis buffer supplemented with protease inhibitors, separated by SDS-PAGE and transferred to a PVDF membrane (Millipore, Billerica, MA). The membrane was blocked with 5% non-fat milk at room temperature for 1 h and incubated with the appropriate antibody. Protein bands were visualized by the chemiluminescent HRP detection system (Millipore, Billerica, MA). The antibodies are listed below: MCM6 (1:2000, Proteintech, Chicago, IL), E-cadherin (1:1000, CST, Boston, MA), Vimentin (1:1000, CST, Boston, MA), GAPDH (1:5000, Ray antibody, China), ZO1 (1:1000, CST, Boston, MA), Fibronectin (1:1000, Wanleibio, China), p-ERK1/2 (1:300, Wanleibio, China), ERK1/2(1:500, Wanleibio, China), p-MEK1/2 (1:500, ABclonal, China), and MEK1/2 (1:1000, Wanleibio, China).
Chemiluminescent hrp detection system
Manufactured by Merck Group
Sourced in Morocco
The Chemiluminescent HRP detection system is a laboratory equipment product that utilizes chemiluminescence to detect and quantify the presence of horseradish peroxidase (HRP) in a sample. It provides a sensitive and reliable method for the detection of HRP-labeled molecules, such as proteins or nucleic acids, in various applications.
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Lab products found in correlation
2 protocols using chemiluminescent hrp detection system
1
Western Blot for Protein Expression Analysis
2
Protein Extraction and Western Blot Analysis
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Total proteins were extracted with RIPA lysis buffer supplemented with protease inhibitors, separated by SDSPAGE, and transferred to a PVDF membrane (Millipore, Billerica, MA). The membrane was blocked with 5% non-fat milk at room temperature for 1 hr and incubated with the appropriate antibody. Protein bands were visualized using the chemiluminescent HRP detection system (Millipore). The antibodies are as follows: ANO6 (1:1000, cat#PA5-69345, ThermoFisher, California, USA), ERK (1:1000, cat#13–6200, ThermoFisher, California, USA), phosphorylation (phospho)-ERK (1:500, cat#44-680G, ThermoFisher, California, USA), LaminB (1:2000, cat#PA5-66473, ThermoFisher, California, USA), β-actin (1:5000, cat#MA5-15739, ThermoFisher, California, USA).
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