Venous blood samples were collected by a well-trained lab technician to determine levels of 25-hydroxyvitamin D, fasting blood glucose (FBG), hemoglobin A1c (HbA1c) and lipid profile. Serum was prepared by centrifuging blood samples at 2100 g for 8 min at room temperature within 1 h of blood sampling using a high speed Jouan centrifuge (Thermo Fisher Scientific, Inc., Waltham, MA, USA).
Jouan centrifuge
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Jouan centrifuge is a laboratory equipment designed for the separation of samples. It utilizes centrifugal force to separate components within a sample, such as cells, organelles, or other particles, based on their size and density. The Jouan centrifuge is suitable for a range of applications in various research and clinical settings.
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Lab products found in correlation
4 protocols using jouan centrifuge
1
Venous Blood Sampling for Vitamin D and Metabolic Markers
2
Serum 25-Hydroxyvitamin D Measurement
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Serum was prepared from fresh venous blood samples by centrifugation at 2,100 g for 8 min at room temperature using a high speed Jouan centrifuge (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Then, concentration of 25-hydroxyvitamin D was measured by electrochemiluminescence immunoassay using Roche Modular E170 Analyzer (Roche Diagnostics, Basel, Switzerland). Participants were classified according to their serum 25-hydroxyvitamin D into normal vitamin D levels (≥30 ng/mL), insufficient vitamin D levels (20-30 ng/mL), deficient vitamin D levels (10-20 ng/mL), or severely deficient (<10 ng/mL) (Holick, 2009 (link)).
3
Serum Vitamin D Measurement Protocol
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Venous blood samples were collected by a well-trained lab technician into anticoagulant-free plain test tubes. Then, serum was prepared by centrifuging blood samples at 2100 g for 8 min at room temperature within 2 h of blood collection using a high-speed Jouan centrifuge (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Serum 25 (OH) D levels were determined by electrochemiluminescence immunoassay using Roche Modular E170 Analyzer (Roche Diagnostics, Basel, Switzerland). Participants were classified according to their serum 25 (OH) D into sufficient vitamin D levels (≥30 ng/mL), insufficient vitamin D levels (20–30 ng/mL), or deficient vitamin D levels (<20 ng/mL)63 (link).
4
Activated Sludge Cultivation and Washing
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Obtaining the inoculated activated sludge was ensured from a local wastewater treatment plant (Rennes Beaurade, France). The activated sludge was kept active in an 8 L bioreactor at room temperature by adding nutrients (g.L -1 ): peptone, 0.64; K 2 HPO 4 , 0.11; NH 4 Cl, 15.2; CH 3 COONa, 140 and an additional source of carbon (viandox). The activated sludge was washed three times with tap water and two times by ultra-pure water. Before each washing the sludge was centrifuged at 4000 rpm for 10 min (Jouan centrifuge, Thermo Fisher Scientifics, Saint Herblain, France). Carbon residues and mineral nutrients present in the activated sludge were removed before their use in the experiments.
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