Str profiling

Manufactured by Leibniz Institute DSMZ

Sourced in Germany

STR profiling is a molecular biology technique used for DNA identification and analysis. It involves the amplification and examination of short tandem repeat (STR) regions within the human genome. This method provides a reliable and standardized way to generate DNA profiles for various applications, such as forensics, paternity testing, and population genetics studies.

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Lab products found in correlation

Hct116, by American Type Culture Collection (1 mentions) Fetal calf serum (fcs), by PAN Biotech (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Auranofin, by Santa Cruz Biotechnology (1 mentions) Fetal calf serum (fcs), by Harvard Bioscience (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Hek293t cells, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Hek293t cell, by American Type Culture Collection (1 mentions) Streptomycin, by BioConcept (1 mentions) Protein g sepharose column, by GE Healthcare (1 mentions) Fungizone, by BioConcept (1 mentions)

3 protocols using str profiling

Colorectal Cancer Cell Line Culture

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HCT‐116 (ATCC American Type Culture Collection, Manassas, VA), Hke3 (kind gift from Dr. Owen Sansom), Hkh2 (kind gift from Prof. Dr. Walter Kolch), DiFi (kind gift from Dr. Clara Montagut) and SW480 (ATCC) cell lines were maintained in DMEM (Dulbecco's‐modified Eagle medium, Sigma‐Aldrich Biochemie GmbH, Taufkirchen, Germany) supplemented with 10% FCS (fetal calf serum; Pan‐Biotech GmbH. Aidenbach, Germany), 2 mM L‐glutamine (Sigma‐Aldrich Biochemie GmbH) and 1% NEAA (nonessential amino acids) (Sigma‐Aldrich Biochemie GmbH), at 37 °C and 5% CO₂ in a humidified incubator.
HT29 cells (ATCC) were maintained in Ham's medium (Sigma‐Aldrich Biochemie GmbH), supplemented with 10% FCS and 1 mM L‐glutamine (Sigma‐Aldrich Biochemie GmbH), at 37 °C and 5% CO₂ in a humidified incubator.
Cell line identity was validated by STR‐profiling (DSMZ, Braunschweig, Germany) and all cell lines were routinely tested for mycoplasma.

Klein C.H., Truxius D.C., Vogel H.A., Harizanova J., Murarka S., Martín‐Gago P, & Bastiaens P.I. (2018). PDEδ inhibition impedes the proliferation and survival of human colorectal cancer cell lines harboring oncogenic KRas. International Journal of Cancer, 144(4), 767-776.

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Culturing Cell Lines for Oxidative Stress

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Murine lung fibroblasts (MLFs) were kindly provided by Harald von Melchner (Frankfurt am Main, Germany) and A549 cells by Ralf Schubert (Frankfurt am Main, Germany). HEK 293T cells were obtained from the American Type Culture Collection (ATCC). Human cell lines were authenticated by STR profiling (DSMZ, Braunschweig, Germany). All cell lines were continuously monitored for mycoplasma contamination and cultured in DMEM medium (Life technologies Inc., Eggenstein, Germany) supplemented with 10% fetal calf serum (Biochrom, Berlin, Germany), 1% penicillin/streptomycin (Invitrogen, Karlsruhe, Germany). Culture medium for HEK 293T cells also contained 1 mM sodium pyruvate (Invitrogen, Karlsruhe, Germany). Cells were kept at 37 °C and 5% CO₂. Chemicals were purchased from Sigma-Aldrich (Merck, Darmstadt, Germany) unless otherwise indicated. Auranofin was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), H₂O₂ from Carl Roth (Karlsruhe, Germany) and Bleomycin (BLEOCIN, Calbiochem, Merck).

Ehrenfeld V., Heusel J.R., Fulda S, & van Wijk S.J. (2020). ATM inhibition enhances Auranofin-induced oxidative stress and cell death in lung cell lines. PLoS ONE, 15(12), e0244060.

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IGROV1 Ovarian Cancer Cell Culture

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IGROV1 human ovarian cancer cells were kindly provided by Dr. Cristina Müller (Center for Radiopharmaceutical Sciences, Paul Scherrer Institute) and analysed by STR profiling (DSMZ, Braunschweig, Germany). IGROV1 cells were maintained in a humidified atmosphere containing 5% CO₂ in RPMI 1640 medium at 37°C. The medium was supplemented with 10% fetal calf serum (FCS), 2 mM glutamine, 100 units/ml penicillin, 100 μg/ml streptomycin and 0.25 μg/ml fungizone (BioConcept, Allschwil, Switzerland). mAb chCE7 is a IgG1-subtype chimeric monoclonal antibody (human κ light chain and human γ1 heavy chain). It was produced in HEK293 cells and purified from cell culture supernatant using a protein G-Sepharose column (GE Healthcare, Glattbrugg, Switzerland) as described by Grünberg et al. [32 (link)]. An unspecific isotype-matched IgG was used as a control for experiments.

Lindenblatt D., Fischer E., Cohrs S., Schibli R, & Grünberg J. (2014). Paclitaxel improved anti-L1CAM lutetium-177 radioimmunotherapy in an ovarian cancer xenograft model. EJNMMI Research, 4, 54.

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