Mouse phycoerythrin pe igg2b isotype control

Manufactured by BioLegend

Mouse phycoerythrin (PE)-IgG2b isotype control is a laboratory reagent used for flow cytometry applications. It serves as a control to establish baseline fluorescence levels when analyzing cell samples stained with PE-conjugated antibodies.

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Lab products found in correlation

Human trustain fcx, by BioLegend (2 mentions) Streptavidin apc, by BioLegend (2 mentions) Recombinant biotinylated rbd, by BioLegend (2 mentions) Novablock, by Thermo Fisher Scientific (2 mentions) Allophycocyanin apc anti pe, by BioLegend (2 mentions) Streptavidin pe, by BioLegend (2 mentions) Aurora cytometer, by Cytek (1 mentions)

Close spelling variants of this product

IgG2b isotype control PE-isotype control Mouse IgG2b control Mouse IgG2b-PE IgG2b-PE Isotype control Phycoerythrin (PE) Mouse IgG2b IgG2b

2 protocols using mouse phycoerythrin pe igg2b isotype control

RBD-Streptavidin Tetramer Flow Cytometry

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Recombinant biotinylated RBD (BioLegend) was reacted with PE-Streptavidin (BioLegend) in a 4:1 molar ratio at 4°C for 2 hours. For flow cytometry studies, PBMCs underwent Fc-blockade with Human TruStain FcX (BioLegend), NovaBlock (Thermo Fisher Scientific), APC-Streptavidin (BioLegend), and mouse phycoerythrin (PE)-IgG2b isotype control (BioLegend) for 10 min at room temperature, followed by allophycocyanin (APC) anti-PE (BioLegend) for 10 min. Then cells were washed twice and stained with the RBD tetramer and antibodies against other surface proteins for 20 min at room temperature in the dark, followed by resuspension in 1% paraformaldehyde and acquisition on a 5-laser Aurora cytometer (Cytek Biosciences).

Samanovic M.I., Cornelius A.R., Gray-Gaillard S.L., Allen J.R., Karmacharya T., Wilson J.P., Wesley Hyman S., Tuen M., Koralov S.B., Mulligan M.J, & Sedaghat Herati R. (2021). Robust immune responses are observed after one dose of BNT162b2 mRNA vaccine dose in SARS-CoV-2 experienced individuals. Science Translational Medicine.

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RBD Tetramer Staining of PBMCs

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Recombinant biotinylated RBD (BioLegend) was reacted with PE-Streptavidin (BioLegend) in a 4:1 molar ratio at 4°C for 2 hours. For flow cytometry studies, PBMCs underwent Fc-blockade with Human TruStain FcX (BioLegend), NovaBlock (Thermo Fisher Scientific), APC-Streptavidin (BioLegend), and mouse phycoerythrin (PE)-IgG2b isotype control (BioLegend) for 10 minutes at room temperature, followed by allophycocyanin (APC) anti-PE (BioLegend) for 10 minutes. Then cells were washed twice and stained with the RBD tetramer and antibodies against other surface proteins for 20 minutes at room temperature in the dark, followed by resuspension in 1% paraformaldehyde and acquisition on a 5-laser Aurora cytometer (Cytek Biosciences).

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