Abi7300 system sds software

Manufactured by Thermo Fisher Scientific

The ABI7300 system SDS software is a tool used for real-time PCR data analysis. It provides basic functionalities to analyze and interpret results from real-time PCR experiments.

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Lab products found in correlation

Sybr green master mix, by Thermo Fisher Scientific (3 mentions) Improm reverse transcription system, by Promega (2 mentions) Improm 2 reverse transcription system, by Promega (1 mentions) Trizol plus rna purification kit, by Thermo Fisher Scientific (1 mentions) Trizol plus rna puri cation kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using abi7300 system sds software

Quantitative Real-Time PCR Analysis

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Total RNAs in cells and tissues were extracted by TRIzol™ Plus RNA purification kit (Invitrogen) and first-strand cDNA was synthesized by the ImProm-II reverse transcription system (Promega). Real-time PCR assays were conducted by the SYBR Green Mastermix (Applied Biosystems) and all procedures were performed according to the manufacturer’s instructions. Specific primers used for genes were as follows: NFAT2 forward, 5′-GCTATGCATCCTCCAACGTC-3′; and reverse, 5′-AGTTFFACTCGTAGGAGGAG-3′; EGR2 forward, 5′-TCAGCATCTCCCAACCTAT-3′; and reverse, 5′-ACAACAAACACTACCACCCT-3′; FASL forward, 5′-GTTCTGGTTGCCTTGGTAG-3′; and reverse, 5′-CATCTGGCTGGTAGACTCT-3′; COX-2 forward, 5′-GAAAGCCCTCTACCATGACATC-3′; and reverse, 5′-CACCCTTTCACATTATTGCAGA-3′; c-myc forward, 5′-GCCACGTCTCCACACATCAG-3′; and reverse, 5′-TCTTGGCAGCAGGATAGTCCTT-3′; β-actin forward, 5′-CTGGGACGACATGGAGAAAA-3′; and reverse, 5′-AAGGAAGGCTGGAAGAGTGC-3′. All data were analyzed by the ABI7300 system SDS software (Applied Biosystems) and the mRNA relative expression was calculated by the 2^-∆∆Ct method. The β-actin expression was regarded as control, so the expression value for each gene was normalized to β-actin expression in each sample.

Wang J., Zhang Y., Liu L., Cui Z., Shi R., Hou J., Liu Z., Yang L., Wang L, & Li Y. (2020). NFAT2 overexpression suppresses the malignancy of hepatocellular carcinoma through inducing Egr2 expression. BMC Cancer, 20, 966.

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Quantitative Gene Expression Analysis

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Total RNAs in cells and tissues were extracted by TRIzol TM Plus RNA puri cation kit (Invitrogen) and rststrand cDNA was synthesized by the ImProm-reverse transcription system (Promega). Real-time PCR assays were conducted by the SYBR Green Mastermix (Applied Biosystems) and all procedures were performed according to the manufacturer's instructions. Speci c primers used for genes were as follows: NFAT2 forward, 5'-GCTATGCATCCTCCAACGTC-3'; and reverse, 5'-AGTTFFACTCGTAGGAGGAG-3'; EGR2 forward, 5'-TCAGCATCTCCCAACCTAT-3'; and reverse, 5'-ACAACAAACACTACCACCCT-3'; FASL forward, 5'-GTTCTGGTTGCCTTGGTAG-3'; and reverse, 5'-CATCTGGCTGGTAGACTCT-3'; COX-2 forward, 5'-GAAAGCCCTCTACCATGACATC-3'; and reverse, 5'-CACCCTTTCACATTATTGCAGA-3'; c-myc forward, 5'-GCCACGTCTCCACACATCAG-3'; and reverse, 5'-TCTTGGCAGCAGGATAGTCCTT-3'; β-actin forward, 5'-CTGGGACGACATGGAGAAAA-3'; and reverse, 5'-AAGGAAGGCTGGAAGAGTGC-3'. All data were analyzed by the ABI7300 system SDS software (Applied Biosystems) and the mRNA relative expression was calculated by the 2 -∆∆Ct method. The β-actin expression was regarded as control, so the expression value for each gene was normalized to β-actin expression in each sample.

Wang J., Zhang X., Liu L., Cui Z., Shi R., Hou J., Liu Z., Yang L., Wang L., & Wang J. (2020). NFAT2 overexpression suppresses the malignancy of hepatocellular carcinoma through inducing Egr2 expression.

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Quantitative RT-PCR for Gene Expression Analysis

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Total RNAs in cells and tissues were extracted by TRIzol™ Plus RNA puri cation kit (Invitrogen) and rststrand cDNA was synthesized by the ImProm-reverse transcription system (Promega). Real-time PCR assays were conducted by the SYBR Green Mastermix (Applied Biosystems) and all procedures were performed according to the manufacturer's instructions. Speci c primers used for genes were as follows: NFAT2 forward, 5'-GCTATGCATCCTCCAACGTC-3'; and reverse, 5'-AGTTFFACTCGTAGGAGGAG-3'; EGR2 forward, 5'-TCAGCATCTCCCAACCTAT-3'; and reverse, 5'-ACAACAAACACTACCACCCT-3'; FASL forward, 5'-GTTCTGGTTGCCTTGGTAG-3'; and reverse, 5'-CATCTGGCTGGTAGACTCT-3'; COX-2 forward, 5'-GAAAGCCCTCTACCATGACATC-3'; and reverse, 5'-CACCCTTTCACATTATTGCAGA-3'; c-myc forward, 5'-GCCACGTCTCCACACATCAG-3'; and reverse, 5'-TCTTGGCAGCAGGATAGTCCTT-3'; β-actin forward, 5'-CTGGGACGACATGGAGAAAA-3'; and reverse, 5'-AAGGAAGGCTGGAAGAGTGC-3'. All data were analyzed by the ABI7300 system SDS software (Applied Biosystems) and the mRNA relative expression was calculated by the 2 -∆∆Ct method. The β-actin expression was regarded as control.

Wang J., Zhang X., Liu L., Cui Z., Shi R., Hou J., Liu Z., Yang L., Wang L., & Li Y. (2020). NFAT2 overexpression suppresses the malignancy of hepatocellular carcinoma through inducing Egr2 expression.

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