Creb d76d11

Primary antibodies for mouse anti-MITF (#sc-25386), tyrosinase (#sc-15341), TRP-1 (#sc-514900), TRP-2 (#sc-74439), p-ERK (#sc-7383), ERK 1/2 (#sc-514302), and β-actin (#sc-47778) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The primary anti-bodies for mouse anti-cyclic AMP (cAMP) response element-binding protein (CREB) (8763) (#9198S) and CREB (D76D11) (#4820S) were purchased from Cell Signaling Technology (Beverly, MA, USA); all primary antibodies were used at 1:1000 dilutions. Secondary mouse antibody for primary antibodies was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and used at 1:5000 dilutions. Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM), and penicillin/streptomycin were purchased from Gibco BRL (Carlsbad, CA, USA). Most chemicals, including α-MSH, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), L-DOPA, 1,1-diphenyl-2-picryl-hydrazy (DPPH), 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), arbutin, and mushroom tyrosinase were purchased from Sigma-Aldrich (St. Louis, MO, USA).