Polyethylene glycol (PEG) 4000 Da (A16151) was purchased from Alpha-Aesar. Bovine serum albumin (BSA) (A7638), Potassium phospate dibasic trihydrate (60349), 3-(Trimethoxysilyl)propylmethacrylate (440159), 2-Hydroxy-4′-(2-hydroxyyethoxy)-2-methylpropiophenone (410896), Poly(ethylene glycol) diacrylate (PEGDA) 700 Da (455008), l -lactate dehydrogenase (LDH) from muscle rabbit (L1254), Jack bean urease (U4002), β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate (NADH) (N8129), Pyruvate (P8524), Lactate assay Kit (MAK064), Phenol nitroprusside solution (P6994), Alkaline hypochlorite solution (A1727), Urease Activity Assay Kit (MAK120), Rhodamine-B (R6626) were purchased from Sigma-Aldrich. Potassium phosphate monobasic (42420) was purchased from Acros Organics. Deuterium oxide (D2O) (DE50B) was purchased from Apollo. N,N-dimethylformamide (DMF) (D119) was purchased from Fisher Chemicals. Alexa Fluor 594 and 488 Microscale Protein Labeling Kit (A30008 and A30006) and Carboxylic acid, Acetate, Succinimidyl Ester SNARF-1 (S2280) were purchased from Thermo Fischer Scientific. Trichloroacetic acid (TCA) (34603) was purchased from Nacalai Tesque. Fluorescent polystyrene nanoparticles tracers of 0.2 and 1 μm of diameter (FCDG003, FCDG006) were purchased from Bangs Laboratories. Fluorescein isothiocyanate (FITC) (AB178737) was purchased from abcr.
Phenol nitroprusside solution
Manufactured by Merck Group
Phenol nitroprusside solution is a colorimetric reagent used for the detection and quantification of ammonia, ammonium ions, and certain other nitrogen-containing compounds in chemical analysis and biochemical applications. It functions by producing a characteristic purple-colored complex upon reaction with the target analytes.
Automatically generated - may contain errors
Lab products found in correlation
Alkaline hypochlorite solution, by Merck Group (3 mentions)
Poly ethylene glycol diacrylate, by Merck Group (1 mentions)
Lactate assay kit, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Thermo Fisher Scientific (1 mentions)
3 trimethoxysilyl propyl methacrylate, by Merck Group (1 mentions)
Pyruvate, by Merck Group (1 mentions)
L lactate dehydrogenase, by Merck Group (1 mentions)
β nicotinamide adenine dinucleotide, by Merck Group (1 mentions)
Potassium phosphate monobasic, by Thermo Fisher Scientific (1 mentions)
Reduced disodium salt hydrate, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Nh4cl, by Merck Group (1 mentions)
3 protocols using phenol nitroprusside solution
1
Comprehensive Biophysical Characterization of PEGylated Hydrogels
2
Serum BUN Measurement Protocol
3
Quantification of Urease Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The assays used to characterize
urease activity were based on the Berthelot method.60 (link),61 (link) Briefly, in a 96-well plate, reactions of 50 μL of urea solubilized
in 1× PBS at a set concentration were mixed with 50 μL
of soluble urease (diluted to a final concentration of 0.5 nM in 1×
PBS) or particles and incubated for 2 min at RT. Wells containing
0–200 μM ammonium chloride solution (NH4Cl,
Sigma-Aldrich, cat. no. 254134) were prepared alongside the assays
in order to make a calibration curve to quantify ammonium production.
To stop the ureolytic reactions, 80 μL of phenol nitroprusside
solution (Sigma-Aldrich, cat. no. P6994) was added to each assay and
calibration standard, followed by 40 μL of an alkaline hypochlorite
solution (Sigma-Aldrich, cat. no. A1727). The plates were mixed thoroughly,
then incubated for 30 min at 37 °C, before reading the absorbance
of the plate at 630 nm. The rate of the urease reaction was determined
using the NH4Cl calibration curve.
urease activity were based on the Berthelot method.60 (link),61 (link) Briefly, in a 96-well plate, reactions of 50 μL of urea solubilized
in 1× PBS at a set concentration were mixed with 50 μL
of soluble urease (diluted to a final concentration of 0.5 nM in 1×
PBS) or particles and incubated for 2 min at RT. Wells containing
0–200 μM ammonium chloride solution (NH4Cl,
Sigma-Aldrich, cat. no. 254134) were prepared alongside the assays
in order to make a calibration curve to quantify ammonium production.
To stop the ureolytic reactions, 80 μL of phenol nitroprusside
solution (Sigma-Aldrich, cat. no. P6994) was added to each assay and
calibration standard, followed by 40 μL of an alkaline hypochlorite
solution (Sigma-Aldrich, cat. no. A1727). The plates were mixed thoroughly,
then incubated for 30 min at 37 °C, before reading the absorbance
of the plate at 630 nm. The rate of the urease reaction was determined
using the NH4Cl calibration curve.
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