Diethylaminoethyl (DEAE) −52 cellulose and Sephadex G-100 were obtained from Whatman. Co. (British). Dialysis tubes were obtained from Guangzhou Dongju Experimental Apparatus Co. Ltd. (Guangzhou, China). Trypsin 1 : 250 from porcine pancreas was purchased from Biosharp Biological Technology Co., Ltd (Hefei, China). Bovine serum albumin (BSA) and vitamin B1 were provided by Hangzhou Sijiqing Biological Engineering Materials Co. Ltd. (Hangzhou, China). All the other chemicals were of analytical grade.
Sephadex g 100
Manufactured by Cytiva
Sourced in Sweden
Sephadex G-100 is a gel filtration media used for size-exclusion chromatography. It is composed of cross-linked dextran beads and is designed for the separation of molecules based on their size or molecular weight. The porous structure of the Sephadex G-100 beads allows smaller molecules to penetrate into the pores, while larger molecules are excluded, leading to their separation.
Automatically generated - may contain errors
Lab products found in correlation
Galacturonic acid, by Yuanye Bio-Technology (1 mentions)
Rhamnose, by Yuanye Bio-Technology (1 mentions)
Glucuronic acid, by Yuanye Bio-Technology (1 mentions)
Deae 52 cellulose, by Cytiva (1 mentions)
Histostain plus kit, by ZSGB-BIO (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Iscript cdna synthesis kit, by Bio-Rad (1 mentions)
Dab kit, by ZSGB-BIO (1 mentions)
Itaq universal sybr green supermix kit, by Bio-Rad (1 mentions)
8 hydroxy 2 deoxyguanosine 8 ohdg, by R&D Systems (1 mentions)
Dimethyl sulfoxide dmso 3 4 5 dimethylthiazol 2 y1 2 5 diphenyltetrazolium bromide mtt trypan, by Merck Group (1 mentions)
Sephacryl s 300 hr, by Cytiva (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Hydroxyl free radical assay kit, by Nanjing Jiancheng (1 mentions)
2 diphenyl 1 picrylhydrazyl dpph, by Merck Group (1 mentions)
Absolute ethanol, by Tianjin Damao (1 mentions)
Potassium bromide, by Tianjin Damao (1 mentions)
N butanol, by Tianjin Damao (1 mentions)
Chloroform, by Tianjin Damao (1 mentions)
D glucose, by LGC (1 mentions)
6 protocols using sephadex g 100
1
Purification and Analysis of Biomolecules
2
Buddleja officinalis Phytochemical Characterization
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Buddleja officinalis was purchased from the Anhui Bozhou Herb Market (Bozhou, China), which was identified by Prof. Qingping Xiong from Huaiyin Institute of Technology, Huai'an, Jiangsu, China.
Dextrans with different molecular weight (Mw) were from American Polymer Standards Co. (Colorado, USA). Monosaccharide (rhamnose, arabinose, fucose, xylose, mannose, glucose, and galactose) and uronic acid (glucuronic acid and galacturonic acid) standards were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The 1-phenyl-3-methyl-5-pyrazolone (PMP) was purchased from Hangzhou Dayangchem Co. Ltd. (Hangzhou, China). DEAE-52 cellulose and SephadexG-100 were purchased from Whatman (Maidstone, Britain) and Amersham (Uppsala, Sweden), respectively. All other reagents used in this study were of analytical grade.
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Buddleja officinalis was purchased from the Anhui Bozhou Herb Market (Bozhou, China), which was identified by Prof. Qingping Xiong from Huaiyin Institute of Technology, Huai'an, Jiangsu, China.
Dextrans with different molecular weight (Mw) were from American Polymer Standards Co. (Colorado, USA). Monosaccharide (rhamnose, arabinose, fucose, xylose, mannose, glucose, and galactose) and uronic acid (glucuronic acid and galacturonic acid) standards were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The 1-phenyl-3-methyl-5-pyrazolone (PMP) was purchased from Hangzhou Dayangchem Co. Ltd. (Hangzhou, China). DEAE-52 cellulose and SephadexG-100 were purchased from Whatman (Maidstone, Britain) and Amersham (Uppsala, Sweden), respectively. All other reagents used in this study were of analytical grade.
3
Characterization of D. huoshanense Bioactive Compounds
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D. huoshanense was collected and propagated in our lab under the conditions as previously described.[15 ] DEAE-cellulose D-52 and Sephadex G-100 were purchased from Amersham Pharmacia Biotech (London, England) and Sigma-Aldrich Co. (St. Louis, MO, USA), respectively. Enzyme linked immunosorbent assay (ELISA) kits for detection of TNF-α, IL-1β, IL-10 and 8-hydroxy-2′-deoxyguanosine (8-OHdG), were purchased from R&D Systems China Co., Ltd. (Shanghai, China). The primary antibodies against CD68 and phosphorylated IκBα (p-IκBα) (Ser 32) were purchased from Biorbyt (Cambridgeshire, United Kingdom) and Santa Cruz (CA, USA), respectively. TRIzol® Reagent was purchased from Invitrogen (Carlsbad, USA). iScript™ cDNA Synthesis kit and iTaq™ Universal SYBR® Green Supermix kit were purchased from BIO-RAD (CA, USA). Histostain™-Plus Kits and DAB kit were purchased from ZSGB-Bio (Beijing, China).
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D. huoshanense was collected and propagated in our lab under the conditions as previously described.[15 ] DEAE-cellulose D-52 and Sephadex G-100 were purchased from Amersham Pharmacia Biotech (London, England) and Sigma-Aldrich Co. (St. Louis, MO, USA), respectively. Enzyme linked immunosorbent assay (ELISA) kits for detection of TNF-α, IL-1β, IL-10 and 8-hydroxy-2′-deoxyguanosine (8-OHdG), were purchased from R&D Systems China Co., Ltd. (Shanghai, China). The primary antibodies against CD68 and phosphorylated IκBα (p-IκBα) (Ser 32) were purchased from Biorbyt (Cambridgeshire, United Kingdom) and Santa Cruz (CA, USA), respectively. TRIzol® Reagent was purchased from Invitrogen (Carlsbad, USA). iScript™ cDNA Synthesis kit and iTaq™ Universal SYBR® Green Supermix kit were purchased from BIO-RAD (CA, USA). Histostain™-Plus Kits and DAB kit were purchased from ZSGB-Bio (Beijing, China).
4
Purification and Characterization of Emblic Fruit Compounds
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Kunming mice (male, 8-12 weeks old, 18.0-20.0 g, License No. SCXK, 2011-0029) were purchased from Experimental Animal Center of Sun Yat-sen University, Guangzhou, Guangdong province, China. All mice were kept at the animal facilities under specific pathogenfree condition until used. Sterile food and water were supplied.
The fresh fruits of P. emblica, at the commercially mature stage, were purchased from Jieyang, Guangdong province, China. The material was identified by Professor R.M. Yu, College of Pharmacy, Jinan University, China. The fruit was dried at 55°C, and the pulp tissues were carefully removed from the seeds.
Diethylaminoethyl (DEAE) cellulose-52 was obtained from Whatman Ltd. (England). Sephadex G-100 and Sephacryl S-300 HR was purchased from Amersham Biosciences (Swenden). Ascorbic acid (vitamin C, Vc), H 2 O 2 , ferrous sulfate (FeSO 4 ), and sulfate acid (H 2 SO 4 ) were obtained from Guangzhou Chemical Reagent Co., Guangzhou, Guangdong province, China. All other reagents were obtained from Sigma Chemical Co. (USA) and all reagents were of analytical grade. Dimethylsulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium-bromide (MTT), trypan, concanavalin A (Con A), penicillin G and streptomycin were obtained from Sigma Chemical Co. (St. Louis, MO, USA).
The fresh fruits of P. emblica, at the commercially mature stage, were purchased from Jieyang, Guangdong province, China. The material was identified by Professor R.M. Yu, College of Pharmacy, Jinan University, China. The fruit was dried at 55°C, and the pulp tissues were carefully removed from the seeds.
Diethylaminoethyl (DEAE) cellulose-52 was obtained from Whatman Ltd. (England). Sephadex G-100 and Sephacryl S-300 HR was purchased from Amersham Biosciences (Swenden). Ascorbic acid (vitamin C, Vc), H 2 O 2 , ferrous sulfate (FeSO 4 ), and sulfate acid (H 2 SO 4 ) were obtained from Guangzhou Chemical Reagent Co., Guangzhou, Guangdong province, China. All other reagents were obtained from Sigma Chemical Co. (USA) and all reagents were of analytical grade. Dimethylsulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium-bromide (MTT), trypan, concanavalin A (Con A), penicillin G and streptomycin were obtained from Sigma Chemical Co. (St. Louis, MO, USA).
5
Evaluation of Antioxidant Capacity in RIN-m5F Cells
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RIN-m5F (ATCC CRL-11605) cells were purchased from the Tongpai Biotechnology Co. Ltd., Shanghai, China. Cytiva (Uppsala, Sweden) provided diethylaminoethyl 52 (DEAE-52) cellulose and Sephadex G-100. Sodium chloride, anthrone, D-glucose, penicillin-streptomycin solution (100×, including penicillin 10 ku/mL and streptomycin 10 mg/mL), phosphate buffer solution (1×, pH 7.2–7.4, 0.01 M), trypsin-EDTA solution, and Total Antioxidant Capacity (T-AOC) Assay Kit were purchased from Beijing Solarbio Science and Tech. Co. (Beijing, China). Gibco RPMI medium 1640 basic (1×) was arranged from ThermoFisher Biochemical Products (Beijing) Co. (Beijing, China). Fetal bovine serum (FBS, Australia Origin) and Cell Counting Kit-8 were arranged by Invigentech Inc. (Irvine, CA, USA). Sigma-Aldrich Co. (St. Louis, MO, USA) provided the 2-diphenyl-1-picrylhydrazyl (DPPH). Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu, China) provided the Hydroxyl Free Radical Assay Kit. Absolute ethanol, chloroform, n-butanol, concentrated sulfuric acid, and potassium bromide were purchased from the Tianjin Damao Chemical Reagent Co. (Tianjin, China). All compounds were analytical grade unless otherwise specified.
6
Characterization of Psoralea corylifolia Extracts
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Psoralea corylifolia Linn. (PC) was purchased from Bozhou Yonggang Pieces Factory Co., Ltd. (Bozhou, China) which was identified by Changqin Li, the professor of pharmaceutical college of Henan University. The voucher specimens were deposited in the herbarium of Huanghe Science and Technology College.
The monosaccharides standard substances (l (+)-rhamnose, l (+)-arabinose, d (+)-xylose, d (+)-mannose, d (+)-glucose, d -galactose) were purchased from the Dr Ehrenstorfer GmbH (Germany). DEAE-52 Cellulose was purchased from Saipuruisi Technology Co., LTD. (Beijing, China). Sephadex G-100 was purchased from GE Healthcare Bio-Science AB (Stockholm, Sweden).
The monosaccharides standard substances (
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