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2 2 diphenyl 1 picrylhydrazyl (dpph)

Manufactured by Nacalai Tesque
Sourced in Japan

DPPH is a chemical compound used as a free radical scavenging agent in various analytical and research applications. It serves as a stable free radical that can be used to measure the antioxidant activity of substances.

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2 protocols using 2 2 diphenyl 1 picrylhydrazyl (dpph)

1

Extraction and Analysis of Ishimozuku and Okinawa-mozuku

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Ishimozuku (Sphaerotrichia divaricata) was harvested in the Fukaura town and Azigasawa town Aomori, Japan (Figure 5). Plant samples were collected in 2014 to 2016 during the summer. Okinawa-mozuku (Cladosiphon okamuranus) that had been harvested in Okinawa was purchased from a market (Aomori city).
Folin–Ciocalteu’s phenol reagent, DPPH, was purchased from Nacalai Tesque Inc. (Kyoto, Japan). Other chemical reagents were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan) or from Nacalai Tesque Inc. (Kyoto, Japan).
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2

Antioxidant Activity of Naphtha-lene Derivatives

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Naphthalene, 1-Nap, ABTS, DPPH, potassium peroxodisulfate, phosphoric acid were products of Nacalai Tesque Inc. (Kyoto, Japan). 1-NapS was from Research Organics Inc. (Cleveland, OH, USA). 1-Methoxynaph-thalene (1-NapM), 2-Nap, and potassium dichromate were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 2-NapS was a product of Alfa Aesar (Ward Hill, MA, USA). 2-Methoxynaphtalene (2-NapM) and CPZ were products of Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) was from Sigma-Aldrich, Co. (St Louis, MO, USA). All other chemicals were of the highest grade commercially available. Figure 1 shows the chemical structures of Nap isomers and their derivatives used in this study.
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