Ten-week-old male mice (n = 28) were acclimated to thermoneutrality (30 °C) for 4 weeks and then single with free access to food and water. The core body temperature prior and during mild cold (RT) or severe cold (4 °C) exposure was monitored using BAT-12 Microprobe Thermometer with probe RET-3 (Physitemp) at the indicated times (15, 30, 60, and 120 min). BAT and tail temperature was recorded with a thermal imaging camera (FLIR i3, FLIR Systems) and analyzed with FLIR QuickReport software. After temperature measurement, mice were euthanized, and iBAT was assayed for Akt/pS473, AKT, ACLY/pS455, ACLY, S6RP/p(S240/244), and phosphor-PKA substrates. In another set of experiment, male mice (n = 12) were acclimated to thermoneutrality (30 °C) for 2–4 weeks. For acute mild cold challenge, mice (n = 4) and for acute severe cold challenge, mice (n = 4) were housed individually, placed at either RT or 4 °C for 1 h, with free access to food and water. Control mice (n = 4) were maintained at thermoneutrality. Mice were euthanized and iBAT was assayed to immunoblot for Akt/pS473, AKT, ACLY/pS455, ACLY, S6RP/p(S240/244), and phosphor-PKA substrates.
Ret 3 probe
Manufactured by Physitemp
Sourced in United States
The RET-3 probe is a temperature measurement device designed for use in a laboratory setting. Its core function is to accurately measure and display the temperature of a sample or object under observation.
Automatically generated - may contain errors
Lab products found in correlation
Temp 10t thermocouple, by Physitemp (2 mentions)
Bat 12 microprobe thermometer, by Physitemp (1 mentions)
Flir i3, by Teledyne (1 mentions)
Bat 12, by Physitemp (1 mentions)
Thermalert th 5 thermometer, by Physitemp (1 mentions)
Prism v7, by GraphPad (1 mentions)
Hair clipper, by Philips (1 mentions)
6 protocols using ret 3 probe
1
Cold Exposure Regulation of Metabolic Pathways
2
Jiadifenolide-Induced Hypothermia in Mice
3
Cold Exposure Study in Mice
4
Cold Exposure Study in Mice
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Baseline measurements of internal core-body temperatures were recorded on 10 week-old male mice maintained at 22°C (starting at ZT 4) using a digital thermometer (Oakton Instruments, Temp 10T Thermocouple) and rectal thermocouple probe (Physitemp, RET-3 probe). Cold-exposure studies were performed within climate-controlled rodent incubators. Mice were placed in pre-chilled cages at 4–5°C with bedding, a cotton nestlet, free access to standard chow and water, and cage lid partially open. Rectal temperatures were recorded every 60 minutes. Individual mice were removed from the study and euthanized if core body temperature fell ≥10°C from baseline measurement. Core temperatures ≥10°C from baseline measurement were not included in core temperature analysis and scored as an event for survival analysis.
5
AIT-Mediated Protection Against Anaphylaxis
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Three weeks after the last AIT session, mice were challenged by an i.p. injection of 10 IR cat dander allergen extract (or 52.4 μg birch pollen allergen extract) to measure AIT-mediated protection against anaphylaxis. Before the challenge, and at 20–30 min intervals thereafter, body temperature was measured rectally with a digital Thermalert TH-5 thermometer with a RET-3 probe (Physitemp, Huron, NJ). A time–temperature curve was plotted and the area under curve (AUC) was calculated in GraphPad Prism v7.02 from GraphPad Software (La Jolla, CA). One day after the challenge, mice were euthanized and spleens were harvested for restimulation of splenocytes and analysis of cytokine secretion.
6
Cardiac Function Assessment in Murine MI
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Cardiac functional parameters of adult mice were assessed at baseline (1 day before induction of MI) and at 2 weeks. Briefly, anesthesia was induced using isoflurane in medical O2 at concentrations of 2%–3%. Isoflurane was used for anesthesia maintenance at around 2%. Rectal temperature was monitored (36.5°C–38°C) with a Physitemp RET-3 probe. Thoracic hair was removed using commercially available electric shavers (Philips Hairclipper, HC9490/15) and depilatory cream (Veet Hair Removal Cream for sensitive skin). Conductive ultrasound gel (Parkers Lab) was then applied to each paw to record ECG signals. Prewarmed Aquasonic 100 ultrasound transmission gel was applied onto the abdomen avoiding bubble formation. Image acquisition was performed on a Visualsonics Vevo 770 ultrasound biomicroscope. Cardiac function was assessed using standard parasternal long-axis images of the LV in M-mode and ECG-gated kilohertz visualization B-mode. Functional measurements were extracted using Vevo 770 software.
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