Log In Sign up
The largest database of trusted experimental protocols

Ki67 vp k451

Manufactured by Vector Laboratories
Visit Supplier

Ki67 (VP-K451) is a laboratory product offered by Vector Laboratories. It is a protein that is associated with cellular proliferation and can be used as a marker to identify dividing cells. The product provides a tool for researchers to study cell growth and division.

Automatically generated - may contain errors

Lab products found in correlation

Pannoramic viewer 1, by 3DHISTECH (2 mentions) Cleaved caspase 3 asp175, by Cell Signaling Technology (2 mentions) Cd3 a0452, by Agilent Technologies (2 mentions) Axioimager z2 system, by Zeiss (2 mentions) Pannoramic scan 250 flash, by 3DHISTECH (2 mentions)

2 protocols using ki67 vp k451

1

Histological Analysis of Organ Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Organ samples were fixed in fresh 4% paraformaldehyde at 4 °C overnight and further subjected to routine histological procedures for embedding in paraffin. 5-μm sections of the samples from at least three different animals per group were placed on microscopic slides next to one another to enable cross-comparison within a slide after H&E staining or IHC staining with the antibodies indicated. Antibodies used were GFP (D5.1) XP, PTEN (D4.3), phospho (p)-AKT (S473) (D9E), p-ribosomal protein S6 (S235/236) (D57.2.2E), Cleaved Caspase-3 (Asp175) (all: Cell Signaling Technologies, Danvers, MA), Ki67 (VP-K451, Vector Laboratories, Burlingame, CA), and CD3 (A0452) (DAKO, Glostrup, Denmark). Stained slides were scanned with a Pannoramic Scan 250 Flash or MIDI system and images acquired using Pannoramic Viewer 1.15.2 (both: 3DHistech, Budapest, Hungary). Additional images were taken on a Zeiss Axio Imager.Z2 system.
Miething C., Scuoppo C., Bosbach B., Appelmann I., Nakitandwe J., Ma J., Wu G., Lintault L., Auer M., Premsrirut P.K., Teruya-Feldstein J., Hicks J., Benveniste H., Speicher M.R., Downing J.R, & Lowe S.W. (2014). PTEN action in leukemia dictated by the tissue microenvironment. Nature, 510(7505), 402-406.
+ Open protocol
+ Expand
2

Histological Analysis of Organ Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Organ samples were fixed in fresh 4% paraformaldehyde at 4 °C overnight and further subjected to routine histological procedures for embedding in paraffin. 5-μm sections of the samples from at least three different animals per group were placed on microscopic slides next to one another to enable cross-comparison within a slide after H&E staining or IHC staining with the antibodies indicated. Antibodies used were GFP (D5.1) XP, PTEN (D4.3), phospho (p)-AKT (S473) (D9E), p-ribosomal protein S6 (S235/236) (D57.2.2E), Cleaved Caspase-3 (Asp175) (all: Cell Signaling Technologies, Danvers, MA), Ki67 (VP-K451, Vector Laboratories, Burlingame, CA), and CD3 (A0452) (DAKO, Glostrup, Denmark). Stained slides were scanned with a Pannoramic Scan 250 Flash or MIDI system and images acquired using Pannoramic Viewer 1.15.2 (both: 3DHistech, Budapest, Hungary). Additional images were taken on a Zeiss Axio Imager.Z2 system.
Miething C., Scuoppo C., Bosbach B., Appelmann I., Nakitandwe J., Ma J., Wu G., Lintault L., Auer M., Premsrirut P.K., Teruya-Feldstein J., Hicks J., Benveniste H., Speicher M.R., Downing J.R, & Lowe S.W. (2014). PTEN action in leukemia dictated by the tissue microenvironment. Nature, 510(7505), 402-406.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!