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Dmk 22buc03

Manufactured by Imaging Source
Sourced in Germany

The DMK 22BUC03 is a USB 3.0 industrial camera from Imaging Source. It features a 1/2.8" CMOS sensor with a resolution of 1920 x 1080 pixels. The camera can capture up to 60 frames per second and supports a variety of image formats, including 8-bit and 10-bit monochrome and color.

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5 protocols using dmk 22buc03

1

Behavioral Monitoring of Mice in Arenas

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After at least 5 d of behavioral training, mice underwent four 5-min recording sessions per day in the four different arenas in a random sequential order. The arenas were cleaned with 70% ethanol prior to each recording session to eliminate any olfactory cues. During the sessions, food pellets were thrown into the arena approximately every 1 min. The excitation light intensity was approximately 0.1 to 0.5 mW/mm2, and fluorescence images were captured at 30 Hz. The focus was adjusted to match the previous image. Animal behavior was recorded at 30 Hz with an overhead camera (DMK22BUC03, The Imaging Source).
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2

Dy0.7Tb0.3FeO3 Single-Crystal Characterization

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Single-crystals of Dy0.7Tb0.3FeO3 were grown by the floating-zone method under O2 atmosphere. Using back-reflection X-ray Laue diffractometry, thin c-oriented plates of 2.5 × 2.5 mm2 were cut, lapped, and polished with a silica slurry down to ~60–80 μm with optically flat surfaces on both sides. Temperature and magnetic field treatment was done in a liquid-helium-cooled magnetic-field cryostat (Oxford Spectromag 4000-10T). For Faraday imaging, a light-emitting diode with a central wavelength of 660 nm (Thorlabs M660L4) was used along with a monochromatic digital camera (The Imaging Source DMK 22BUC03) for image acquisition. Image processing is limited to adjustments of brightness, contrast and levels and homogeneously applied to the entire image, unless noted. The images of each figure were adjusted equally. For electric field measurements, 3-nm platinum electrodes were deposited on both surfaces of the specimen.
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3

Photoresponses of Larval Organisms

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Photoresponses of larvae of different ages were assayed in a vertical Plexiglas column (31 mm x 10 mm x 160 mm water height). The column was illuminated from above with light from a monochromator (Polychrome II, Till Photonics). The monochromator was controlled by AxioVision 4.8.2.0 (Carl Zeiss MicroImaging GmbH) via analog voltage or by a custom written Java program via the serial port. The light passed a collimator lens (LAG-65.0–53.0 C with MgF2 Coating, CVI Melles Griot). The column was illuminated from both sides with light-emitting diodes (LEDs). The LEDs on each side were grouped into two strips. One strip contained UV (395 nm) LEDs (SMB1W-395, Roithner Lasertechnik) and the other infrared (810 nm) LEDs (SMB1W-810NR-I, Roithner Lasertechnik). The UV LEDs were run at 4 V to stimulate the larvae in the column from the side. The infrared LEDs were run at 8 V (overvoltage) to illuminate the larvae for the camera (DMK 22BUC03, The Imaging Source), which recorded videos at 15 frames per second and was controlled by IC Capture (The Imaging Source).
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4

Synchronized Body Movement Monitoring

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Simultaneous to wide-field imaging, we recorded movements of the body of the mouse during the task at 30 Hz (Body camera, The Imaging Source; DMK 22BUC03; 720x480 pixels). As imaging was performed in darkness, we illuminated the mouse using a 940-nm infrared LED.
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5

Behavioral Tracking in Mice

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After at least 5 days of behavioral training, mice underwent four 5-min recording sessions per day in the four different arenas in a random sequential order. The arenas 10 were cleaned with 70% ethanol prior to each recording session to eliminate any olfactory cues. During the sessions, food pellets were thrown into the arena approximately every 1 min. The excitation light intensity was approximately 0.1-0.5 mW/mm 2 , and fluorescence images were captured at 30 Hz. The focus was adjusted to match the previous image. Animal behavior was recorded at 30 Hz with an overhead camera (DMK22BUC03, The Imaging Source, Bremen, Germany).
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